PRIMER_SEQUENCE_ID=sN11902
PRIMER_COMMENT=3831
SEQUENCE=ACAGCAACATCGAACTCGTCGTCTTCGAAAGCCTCTTTGGCCTTCGCTGCAAGCTTCTTAGCCATGTCGATTGTGGTTTTTGGTTAGGTTGAGAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGATGATGATTCGATTCAAGAAAGGGAATGAAGTAGTAGACGAGGGCCAAAGAGTATAAAGATGGATGCTATCTTTTTCTCTTCTATTGTTCTGTTATTGTTTTGTGAGGGAAGTTTTGGCGCTTCAATACAAAAAAATAAATAAAGAATAAACAAATACAGCTTAGTTAAATGGGTATAACATGGGCCGGAATATATAATCACGAATTAAGGCCCATCATTGTCTCAAAATTAAACTCGGCCTCTATAATACTTCTCCCACACCGGTAATCTTGAGATGAACACTCCTCTTTCGTGTAGTATAAAATAAGACGAAGGTCCAACGTTGAAAGATACTTACCTGGACGGGGTCTAGTCCACGCGTGGACTAAACTTGAGGACCAACGAAGAAGAAGAAGAAGAAGAAGAAGAAGAGAGTCGTAAAGTGGAAGCAGCGATGAAGAGTAACGATAGTAGTAAGAACAGAGTGAGTTGGTTTATAGATGAAGATCCGTCTCCACGTCCTCCGAAATGCACAGTTCTTTGGAAAGAGCTTTTGCGTTTGAAGAAACAGAGGAGCTCTTCGTCGGTGAAGTCCTCTCTGTCTCCGTCGTCGTCCACGTCATCATCTAGCTCGCTTGAGGATAAGAGAGAAGGGAAGAGAGGTAAGAAAGGATTAGAGAGGACGAGATCGTC
TARGET=513,26
PRIMER_PRODUCT_SIZE_RANGE=100-500
PRIMER_FILE_FLAG=0
PRIMER_LIBERAL_BASE=1
PRIMER_NUM_RETURN=1
PRIMER_FIRST_BASE_INDEX=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 4849, GC content failed 207, low tm 2062, high tm 1173, long poly-x seq 11, ok 1396
PRIMER_RIGHT_EXPLAIN=considered 2415, low tm 892, high tm 844, high end compl 3, ok 676
PRIMER_PAIR_EXPLAIN=considered 2, high end compl 1, ok 1
PRIMER_PAIR_PENALTY=0.0991
PRIMER_LEFT_PENALTY=0.095506
PRIMER_RIGHT_PENALTY=0.003589
PRIMER_LEFT_SEQUENCE=CACGCGTGGACTAAACTTGA
PRIMER_RIGHT_SEQUENCE=AACGCAAAAGCTCTTTCCAA
PRIMER_LEFT=484,20
PRIMER_RIGHT=668,20
PRIMER_LEFT_TM=59.904
PRIMER_RIGHT_TM=59.996
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.000
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=185
=
PRIMER_SEQUENCE_ID=sN11926
PRIMER_COMMENT=3854
SEQUENCE=ACGCTGCTCGGAGAAGCGGCGCCGATATCAATCCGTCAGAAAATGTTTGTTTTCTTCTTCTTCTTCTTCTTCTTCTTCTCGATCTTTGTGTGTGTGTTTCTTTTAGATCTGACTTTGTAGTTTTAGGTTTGATTGGGCGATTGAATCGAGATGAGTTTTGTTCATTTAAGTTTCAGTTGCCGACACTGACGTAGAGATTTGTGTTAGGGTCCTCGTTTGTTGTGAATTTCAATTGACAAATCTGTTTTGGACATCTAACAAAGGGCTTTGTTTTTGTTTTTGTTGCATATGTAGTCAATGCTGGAACCAACAAAGCGGCTTCGAGCAGCACCTCCTTGAACACAAAGAGGCTCGATGATGATACTGAGACCTTAGCTCGTGAGTATATATAATTCATCTATTCTCTCATTCACAGCGAATTAGTATCGACTTCTATATCTCTCTGTGGTTCAAATAAACTTTAGTTGTATCTTCAAAGCTTTATATACTGTTTTTCTTTCCCGTGTTTAAGAGATTGATTAGTATATTGCTTGTTCGAAGAGTCTTTGTT
TARGET=54,23
PRIMER_PRODUCT_SIZE_RANGE=100-500
PRIMER_FILE_FLAG=0
PRIMER_LIBERAL_BASE=1
PRIMER_NUM_RETURN=1
PRIMER_FIRST_BASE_INDEX=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 315, low tm 52, high tm 212, ok 51
PRIMER_RIGHT_EXPLAIN=considered 4504, GC content failed 63, low tm 2423, high tm 832, ok 1186
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=1.0219
PRIMER_LEFT_PENALTY=0.973324
PRIMER_RIGHT_PENALTY=0.048588
PRIMER_LEFT_SEQUENCE=GCCGATATCAATCCGTCAGA
PRIMER_RIGHT_SEQUENCE=CTCTACGTCAGTGTCGGCAA
PRIMER_LEFT=21,20
PRIMER_RIGHT=196,20
PRIMER_LEFT_TM=60.973
PRIMER_RIGHT_TM=60.049
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=55.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=10.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=176
=
PRIMER_SEQUENCE_ID=sN1922
PRIMER_COMMENT=3922
SEQUENCE=ACAGCAACATCGAACTCGTCGTCTTCGAAAGCCTCTTTGGCCTTCGCTGCAAGCTTCTTAGCCATGTCGATTGTGGTTTTTGGTTAGGTTGAGAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGATGATGATTCGATTCAAGAAAGGGAATGAAGTAGTAGACGAGGGCCAAAGAGTATAAAGATGGATGCGATCTTTTTCTCTTCTATTGTTCTGTTATTGTTTTGTGAGGGAAGTTTTGGCGCTTCAATACAAAAAAATAAATAAAGAATAAACAAATACAGCTTAGTTAAATGGGTATAACATGGGCCGGAATATATAATCACGGATTAAGGCCCATCATTGTCTCAAAATTAAACTCGGCCTCTATAATACTTCTCCCACACCGGTAATCTTGAGATGAACACTCCTCTTTCGTGTAGTATAAAATAAGACGAAGGTCCAACGTTGAAAGATACTTACCTGGACGGGGTC
TARGET=96,32
PRIMER_PRODUCT_SIZE_RANGE=100-500
PRIMER_FILE_FLAG=0
PRIMER_LIBERAL_BASE=1
PRIMER_NUM_RETURN=1
PRIMER_FIRST_BASE_INDEX=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 735, low tm 100, high tm 441, ok 194
PRIMER_RIGHT_EXPLAIN=considered 3232, GC content failed 191, low tm 1479, high tm 573, long poly-x seq 12, ok 977
PRIMER_PAIR_EXPLAIN=considered 3, high end compl 1, ok 2
PRIMER_PAIR_PENALTY=0.2733
PRIMER_LEFT_PENALTY=0.124828
PRIMER_RIGHT_PENALTY=0.148509
PRIMER_LEFT_SEQUENCE=CGTCTTCGAAAGCCTCTTTG
PRIMER_RIGHT_SEQUENCE=CAACGTTGGACCTTCGTCTT
PRIMER_LEFT=20,20
PRIMER_RIGHT=454,20
PRIMER_LEFT_TM=60.125
PRIMER_RIGHT_TM=60.149
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=435
=