http://togogenome.org/gene/565042:BLLJ_RS09570 ^@ http://purl.uniprot.org/uniprot/A0A3R0A696 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the glycosyl hydrolase 43 family.|||Cell membrane|||Involved in the type II arabinogalactan (AG) side chains degradation (PubMed:30564851). Releases arabinofuranose (Araf) from alpha-1,3-Araf-substituted beta-1,6-galactooligosaccharides (PubMed:30564851). Can use radish root AGP, larch AG and arabinan. Shows weaker activity with gum arabic and arabinoxylan (PubMed:30564851). http://togogenome.org/gene/565042:BLLJ_RS08355 ^@ http://purl.uniprot.org/uniprot/E8MF10 ^@ Function|||Similarity|||Subunit ^@ Belongs to the NAD(P)-dependent epimerase/dehydratase family.|||Homodimer.|||Involved in the metabolism of galactose. Catalyzes the conversion of UDP-galactose (UDP-Gal) to UDP-glucose (UDP-Glc) through a mechanism involving the transient reduction of NAD. Can also epimerize UDP-GalNAc to UDP-GlcNAc. Involved in the lacto-N-biose I/galacto-N-biose (LNB/GNB) degradation pathway, which is important for host intestinal colonization by bifidobacteria. http://togogenome.org/gene/565042:BLLJ_RS07765 ^@ http://purl.uniprot.org/uniprot/P0DW94 ^@ Function|||Subcellular Location Annotation|||Subunit ^@ Chaperone required for active expression of the lacto-N-biosidase LnbX.|||Homodimer.|||Secreted http://togogenome.org/gene/565042:BLLJ_RS08365 ^@ http://purl.uniprot.org/uniprot/E8MF12 ^@ Function|||Similarity ^@ Belongs to the protein kinase superfamily.|||Phosphorylates both N-acetylglucosamine (GlcNAc) and N-acetylgalactosamine (GalNAc) at similar rates. Involved in the lacto-N-biose I/galacto-N-biose (LNB/GNB) degradation pathway, which is important for host intestinal colonization by bifidobacteria. Also accepts GTP and ITP as phosphate donors. In vitro, can phosphorylate several GlcNAc and GalNAc derivatives. http://togogenome.org/gene/565042:BLLJ_RS01095 ^@ http://purl.uniprot.org/uniprot/E8MGH9 ^@ Function|||PTM|||Similarity|||Subcellular Location Annotation ^@ Belongs to the glycosyl hydrolase 121 family.|||Beta-L-arabinobiosidase that removes L-arabinofuranose-beta-1,2-L-arabinofuranose disaccharide from various substrates such as carrot extensin and potato lectin. Also acts on L-arabinofuranose (Ara)-beta-1,2-Ara-beta-1,2-Ara-beta-Hyp (Ara(3)-Hyp) but not on Ara-beta-1,3-Ara-beta-1,2-Ara-beta-1,2-Ara-beta--Hyp (Ara(4)-Hyp) or Ara-beta-1,2-Ara-beta-Hyp (Ara(2)-Hyp), suggesting a specificity for unmodified Ara(3)-Hyp substrate. In the presence of 1-alkanols, shows transglycosylation activity, retaining the anomeric configuration of the arabinofuranose residue.|||Membrane|||Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. http://togogenome.org/gene/565042:BLLJ_RS06645 ^@ http://purl.uniprot.org/uniprot/E8ME30 ^@ Activity Regulation|||Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Allosterically activated by fructose 1,6-bisphosphate (FBP).|||Belongs to the LDH/MDH superfamily. LDH family.|||Catalyzes the conversion of lactate to pyruvate.|||Cytoplasm|||Homotetramer. http://togogenome.org/gene/565042:BLLJ_RS08360 ^@ http://purl.uniprot.org/uniprot/E8MF11 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the galactose-1-phosphate uridylyltransferase type 2 family.|||Cytoplasm|||Transfers the UMP unit from UDP-glucose (UDP-Glc) to Gal1P. Can also transfer the UMP unit to GlcNAc1P and GalNAc1P. Involved in the general galactose metabolism, and also involved in the lacto-N-biose I/galacto-N-biose (LNB/GNB) degradation pathway, which is important for host intestinal colonization by bifidobacteria. http://togogenome.org/gene/565042:BLLJ_RS08370 ^@ http://purl.uniprot.org/uniprot/E8MF13 ^@ Function|||Similarity|||Subunit ^@ Belongs to the glycoside hydrolase 112 family.|||Homodimer.|||Reversibly phosphorolyzes lacto-N-biose to Gal1-P and N-acetylglucosamine (GlcNAc) and galacto-N-biose to Gal1-P and N-acetylgalactosamine (GalNAc). Involved in the lacto-N-biose I/galacto-N-biose (LNB/GNB) degradation pathway, which is important for host intestinal colonization by bifidobacteria. http://togogenome.org/gene/565042:BLLJ_RS01090 ^@ http://purl.uniprot.org/uniprot/E8MGH8 ^@ Activity Regulation|||Caution|||Cofactor|||Function|||Similarity|||Subunit ^@ Belongs to the glycosyl hydrolase 127 family.|||Beta-L-arabinofuranosidase that removes the beta-L-arabinofuranose residue from the non-reducing end of various substrates, including beta-L-arabinofuranosyl-hydroxyproline (Ara-Hyp), Ara-beta-1,2-Ara-beta-Hyp (Ara(2)-Hyp), Ara-beta-1,2-Ara-beta-1,2-Ara-beta-Hyp (Ara(3)-Hyp), and beta-L-arabinofuranosyl-(1->2)-1-O-methyl-beta-L-arabinofuranose. In the presence of 1-alkanols, shows transglycosylation activity, retaining the anomeric configuration of the arabinofuranose residue.|||Homodimer in solution.|||Strongly inhibited in the presence of thiol modifiers, suggesting a crucial role for cysteine residues in catalysis. Slightly inhibited by EDTA.|||The original article describing the function has been retracted because the results of E338A and E366A mutants were reversed. The authors later submitted a corrected manuscript.|||Zn(2+) ion is involved in the catalytic reaction through maintaining the proper configuration of active site. http://togogenome.org/gene/565042:BLLJ_RS05690 ^@ http://purl.uniprot.org/uniprot/E8MJ15 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the LDH/MDH superfamily. LDH family.|||Catalyzes the conversion of lactate to pyruvate.|||Cytoplasm|||Homotetramer. http://togogenome.org/gene/565042:BLLJ_RS09505 ^@ http://purl.uniprot.org/uniprot/A0A401ETL2 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the glycosyl hydrolase 30 family.|||Cell membrane|||Involved in the type II arabinogalactan (AG) side chains degradation (PubMed:30564851). Specifically releases the non-reducing terminal beta-1,6-galactobiose (beta-1,6-Gal2) from both dearabinosylated larch AG and polymeric beta-1,6-galactan chains by an exo-mode of action (PubMed:30564851). Shows lower activity with larch AG, and very weak activity with dearabinosylated gum arabic, gum arabic and potato galactan (PubMed:30564851). Can probably release beta-1,6-Gal2 from the internal side chains of type II AG (PubMed:30564851). http://togogenome.org/gene/565042:BLLJ_RS10055 ^@ http://purl.uniprot.org/uniprot/P0DW93 ^@ Activity Regulation|||Cofactor|||Disruption Phenotype|||Domain|||Function|||Miscellaneous|||PTM|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the glycosyl hydrolase 136 (GH136) family.|||Binds 1 Mg(2+) ion per subunit. Magnesium ions are required for efficient protein folding.|||Binds 3 Ca(2+) ions per subunit. Calcium ions are required for efficient protein folding.|||Breast-fed infants have gut microbiota that are substantially more rich in bifidobacteria compared with the mix-fed infants. Notably, B.longum was found to significantly dominate among members of the genus Bifidobacterium in the breast-fed infants.|||Cell membrane|||Cells lacking this gene lose the ability to grow on LNT but continue to grow well on lactose and LNB.|||HMOs function as prebiotics, promoting the growth of bifidobacteria in the gastrointestinal tracts of breast-fed infants, which in turn promotes optimal health.|||Homodimer.|||Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.|||Present in the infant gut, this enzyme is involved in the assimilation of type-1 human milk oligosaccharides (HMOs) (PubMed:23843461, PubMed:28392148). It hydrolyzes via a retaining mechanism the beta-D-GlcNAc-(1->3)-beta-D-Gal linkage in lacto-N-tetraose (LNT or beta-D-Gal-(1->3)-beta-D-GlcNAc-(1->3)-beta-D-Gal-(1->4)-D-Glc), an abundant HMO unique to human breast milk, releasing lacto-N-biose (LNB or beta-D-Gal-(1->3)-D-GlcNAc) and lactose. With a much lower efficiency, is also able to cleave the same linkage in lacto-N-fucopentaose I (alpha-Fuc(1->2)-beta-D-Gal-(1->3)-beta-D-GlcNAc(1->3)-beta-D-Gal-(1->4)-D-Glc), and sialyllacto-N-tetraose a (alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-beta-D-GlcNAc-(1->3)-beta-D-Gal-(1->4)-D-Glc) (PubMed:23843461). Is a key enzymatic factor for growth and proliferation of B.longum in the gut ecosystem of breast-fed infants (PubMed:28392148).|||Requires the chaperone LnbY for its proper folding and active expression (PubMed:23843461). Is potently and competitively inhibited by LNB-PUGNAc and LNB-NHAcDNJ in vitro (PubMed:28392148).|||The region of amino acids 31-625 was identified as the minimum catalytic unit exhibiting full activity.