http://togogenome.org/gene/300852:TTH_RS04520 ^@ http://purl.uniprot.org/uniprot/Q9LCX4 ^@ Function|||Similarity|||Subunit ^@ Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln).|||Belongs to the GatC family.|||Heterotrimer of A, B and C subunits. http://togogenome.org/gene/300852:TTH_RS01630 ^@ http://purl.uniprot.org/uniprot/Q5SLI2 ^@ Cofactor|||Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the cytochrome c oxidase subunit 2 family.|||Binds a copper A center.|||Cell membrane|||Membrane|||Subunits I and II form the functional core of the enzyme complex. Electrons originating in cytochrome c are transferred via heme a and Cu(A) to the binuclear center formed by heme a3 and Cu(B). http://togogenome.org/gene/300852:TTH_RS09235 ^@ http://purl.uniprot.org/uniprot/Q5SHB1 ^@ Function|||Similarity ^@ Belongs to the 2H phosphoesterase superfamily. ThpR family.|||Hydrolyzes RNA 2',3'-cyclic phosphodiester to an RNA 2'-phosphomonoester. http://togogenome.org/gene/300852:TTH_RS09170 ^@ http://purl.uniprot.org/uniprot/O50606 ^@ Cofactor|||Function|||Similarity|||Subunit ^@ Belongs to the FPG family.|||Binds 1 zinc ion per subunit.|||Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates (By similarity).|||Monomer. http://togogenome.org/gene/300852:TTH_RS00105 ^@ http://purl.uniprot.org/uniprot/Q5SMC5 ^@ Function|||Similarity ^@ Belongs to the glycosyltransferase 1 family. Bacterial/plant glycogen synthase subfamily.|||Synthesizes alpha-1,4-glucan chains using ADP-glucose. http://togogenome.org/gene/300852:TTH_RS04515 ^@ http://purl.uniprot.org/uniprot/Q5SJX7 ^@ Domain|||Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.|||Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec).|||Consists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding.|||Cytoplasm|||Homodimer. The tRNA molecule binds across the dimer. http://togogenome.org/gene/300852:TTH_RS09530 ^@ http://purl.uniprot.org/uniprot/Q5SH50 ^@ Similarity ^@ Belongs to the Nudix hydrolase family. http://togogenome.org/gene/300852:TTH_RS01820 ^@ http://purl.uniprot.org/uniprot/Q5SLE7 ^@ Similarity ^@ Belongs to the FKBP-type PPIase family. http://togogenome.org/gene/300852:TTH_RS09585 ^@ http://purl.uniprot.org/uniprot/Q5SH40 ^@ Similarity ^@ Belongs to the peptidase M29 family. http://togogenome.org/gene/300852:TTH_RS01040 ^@ http://purl.uniprot.org/uniprot/Q5SLU4 ^@ Similarity ^@ Belongs to the type-B carboxylesterase/lipase family. http://togogenome.org/gene/300852:TTH_RS04115 ^@ http://purl.uniprot.org/uniprot/Q5SK57 ^@ Subcellular Location Annotation ^@ Nucleus http://togogenome.org/gene/300852:TTH_RS04960 ^@ http://purl.uniprot.org/uniprot/Q5SJN2 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the binding-protein-dependent transport system permease family.|||Cell membrane http://togogenome.org/gene/300852:TTH_RS10045 ^@ http://purl.uniprot.org/uniprot/Q53WE6 ^@ Function|||Similarity ^@ Antitoxin component of a type II toxin-antitoxin (TA) system.|||Belongs to the phD/YefM antitoxin family. http://togogenome.org/gene/300852:TTH_RS06420 ^@ http://purl.uniprot.org/uniprot/Q5SIU8 ^@ Function|||Similarity ^@ Belongs to the helicase family. RecG subfamily.|||Critical role in recombination and DNA repair. Helps process Holliday junction intermediates to mature products by catalyzing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with a 3'- to 5'- polarity. Unwinds branched duplex DNA (Y-DNA). http://togogenome.org/gene/300852:TTH_RS05540 ^@ http://purl.uniprot.org/uniprot/Q5SJC6 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the FtsA/MreB family.|||Cell division protein that is involved in the assembly of the Z ring. May serve as a membrane anchor for the Z ring.|||Cell inner membrane|||Self-interacts. Interacts with FtsZ. http://togogenome.org/gene/300852:TTH_RS02215 ^@ http://purl.uniprot.org/uniprot/Q60049 ^@ Cofactor|||Function|||Similarity|||Subunit ^@ Belongs to the nitroreductase family.|||Binds 1 FMN per subunit.|||Can oxidize either NADH or NADPH with a preference for NADH. Can catalyze electron transfer from NADH to various electron acceptors which include, in addition to molecular oxygen, cytochrome c, 2,6 dichlorphenolindophenol, methylene blue, ferricyanide or P-nitroblue tetrazolium.|||Homodimer. http://togogenome.org/gene/300852:TTH_RS02775 ^@ http://purl.uniprot.org/uniprot/Q5SKW1 ^@ Caution|||Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the sigma-70 factor family. RpoD/SigA subfamily.|||Cytoplasm|||Interacts transiently with the RNA polymerase catalytic core.|||Lacks conserved residue(s) required for the propagation of feature annotation.|||Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the primary sigma factor during exponential growth. http://togogenome.org/gene/300852:TTH_RS09550 ^@ http://purl.uniprot.org/uniprot/Q5SH46 ^@ Similarity ^@ Belongs to the AAA ATPase family. RarA/MGS1/WRNIP1 subfamily. http://togogenome.org/gene/300852:TTH_RS08505 ^@ http://purl.uniprot.org/uniprot/Q5SHP9 ^@ Function|||Similarity|||Subunit ^@ Belongs to the universal ribosomal protein uL24 family.|||One of the proteins that surrounds the polypeptide exit tunnel on the outside of the subunit.|||One of two assembly initiator proteins, it binds directly to the 5'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit.|||Part of the 50S ribosomal subunit. http://togogenome.org/gene/300852:TTH_RS03595 ^@ http://purl.uniprot.org/uniprot/Q5SKF2 ^@ Similarity ^@ Belongs to the short-chain dehydrogenases/reductases (SDR) family. http://togogenome.org/gene/300852:TTH_RS10290 ^@ http://purl.uniprot.org/uniprot/Q53WA2 ^@ Similarity ^@ Belongs to the precorrin methyltransferase family. http://togogenome.org/gene/300852:TTH_RS00705 ^@ http://purl.uniprot.org/uniprot/Q5SM09 ^@ Cofactor|||Function|||Similarity ^@ Belongs to the NrdR family.|||Binds 1 zinc ion.|||Negatively regulates transcription of bacterial ribonucleotide reductase nrd genes and operons by binding to NrdR-boxes. http://togogenome.org/gene/300852:TTH_RS02205 ^@ http://purl.uniprot.org/uniprot/Q5SL70 ^@ Similarity ^@ Belongs to the ComB family. http://togogenome.org/gene/300852:TTH_RS07360 ^@ http://purl.uniprot.org/uniprot/Q5SIC0 ^@ Similarity ^@ Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily. http://togogenome.org/gene/300852:TTH_RS08565 ^@ http://purl.uniprot.org/uniprot/Q5SHN7 ^@ Function|||Similarity|||Subunit ^@ Belongs to the universal ribosomal protein uS10 family.|||Involved in the binding of tRNA to the ribosomes (By similarity). Part of the top of the 30S subunit head.|||Part of the 30S ribosomal subunit. Forms a tight complex with proteins S3 and S14. Also contacts protein S9. http://togogenome.org/gene/300852:TTH_RS05480 ^@ http://purl.uniprot.org/uniprot/Q5SJD8 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the methyltransferase superfamily. RsmH family.|||Cytoplasm|||Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA. http://togogenome.org/gene/300852:TTH_RS07045 ^@ http://purl.uniprot.org/uniprot/Q5SIH8 ^@ Caution|||Function|||Similarity|||Subunit ^@ Associates with the 50S ribosomal subunit.|||Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. EngA (Der) GTPase family.|||GTPase that plays an essential role in the late steps of ribosome biogenesis.|||Lacks conserved residue(s) required for the propagation of feature annotation. http://togogenome.org/gene/300852:TTH_RS09395 ^@ http://purl.uniprot.org/uniprot/Q5SH78 ^@ Cofactor|||Similarity ^@ Belongs to the peptidase M3 family.|||Binds 1 zinc ion. http://togogenome.org/gene/300852:TTH_RS06725 ^@ http://purl.uniprot.org/uniprot/Q5SIP1 ^@ Similarity ^@ Belongs to the 5'-nucleotidase family. http://togogenome.org/gene/300852:TTH_RS06770 ^@ http://purl.uniprot.org/uniprot/Q5SIN1 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the binding-protein-dependent transport system permease family.|||Cell membrane|||Membrane http://togogenome.org/gene/300852:TTH_RS05790 ^@ http://purl.uniprot.org/uniprot/Q5SJ74 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ An RNase that has 5'-3' exonuclease and possibly endonuclease activity. Involved in maturation of rRNA and in some organisms also mRNA maturation and/or decay.|||Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. Bacterial RNase J subfamily.|||Cytoplasm|||Homodimer, may be a subunit of the RNA degradosome. http://togogenome.org/gene/300852:TTH_RS01680 ^@ http://purl.uniprot.org/uniprot/Q5SLH5 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the gluconeogenesis factor family.|||Cytoplasm|||Required for morphogenesis under gluconeogenic growth conditions. http://togogenome.org/gene/300852:TTH_RS02420 ^@ http://purl.uniprot.org/uniprot/Q5SL28 ^@ Cofactor|||Similarity|||Subunit ^@ Belongs to the ferredoxin--NADP reductase type 2 family.|||Binds 1 FAD per subunit.|||Homodimer. http://togogenome.org/gene/300852:TTH_RS09285 ^@ http://purl.uniprot.org/uniprot/Q5SHA1 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the YqgF nuclease family.|||Could be a nuclease involved in processing of the 5'-end of pre-16S rRNA.|||Cytoplasm http://togogenome.org/gene/300852:TTH_RS09225 ^@ http://purl.uniprot.org/uniprot/Q5SHB3 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the RNase Y family.|||Cell membrane|||Endoribonuclease that initiates mRNA decay. http://togogenome.org/gene/300852:TTH_RS08550 ^@ http://purl.uniprot.org/uniprot/Q5SHP0 ^@ Function|||Similarity|||Subunit ^@ Belongs to the universal ribosomal protein uL23 family.|||Contacts protein L29, and trigger factor when it is bound to the ribosome (By similarity). Part of the 50S ribosomal subunit.|||One of the early assembly proteins it binds 23S rRNA. One of the proteins that surrounds the polypeptide exit tunnel on the outside of the ribosome. Forms the main docking site for trigger factor binding to the ribosome. http://togogenome.org/gene/300852:TTH_RS07650 ^@ http://purl.uniprot.org/uniprot/Q5SI63 ^@ Similarity ^@ Belongs to the intradiol ring-cleavage dioxygenase family. http://togogenome.org/gene/300852:TTH_RS06690 ^@ http://purl.uniprot.org/uniprot/Q5SIP8 ^@ Function|||Similarity ^@ Belongs to the PurU family.|||Catalyzes the hydrolysis of 10-formyltetrahydrofolate (formyl-FH4) to formate and tetrahydrofolate (FH4). http://togogenome.org/gene/300852:TTH_RS05775 ^@ http://purl.uniprot.org/uniprot/Q5SJ77 ^@ Subcellular Location Annotation ^@ Membrane http://togogenome.org/gene/300852:TTH_RS06590 ^@ http://purl.uniprot.org/uniprot/Q5SIR3 ^@ Similarity ^@ Belongs to the BMP lipoprotein family. http://togogenome.org/gene/300852:TTH_RS08825 ^@ http://purl.uniprot.org/uniprot/Q5SHI7 ^@ Similarity ^@ Belongs to the OMP decarboxylase family. Type 2 subfamily. http://togogenome.org/gene/300852:TTH_RS08685 ^@ http://purl.uniprot.org/uniprot/Q5SHL6 ^@ Cofactor|||Function|||Similarity|||Subunit ^@ Belongs to the ChdC family. Type 1 subfamily.|||Fe-coproporphyrin III acts as both substrate and redox cofactor (By similarity). Was originally thought to use heme as a cofactor (PubMed:15965735).|||Homopentamer.|||Involved in coproporphyrin-dependent heme b biosynthesis. Catalyzes the decarboxylation of Fe-coproporphyrin III (coproheme) to heme b (protoheme IX), the last step of the pathway. The reaction occurs in a stepwise manner with a three-propionate harderoheme intermediate (By similarity). When reconstituted with heme, can generate oxygen using chlorite or hydrogen peroxide as substrate (in vitro), but has very low affinity for hydrogen peroxide and chlorite and extremely low enzyme activity (PubMed:15965735). http://togogenome.org/gene/300852:TTH_RS02670 ^@ http://purl.uniprot.org/uniprot/Q5SKY1 ^@ Similarity ^@ Belongs to the glycosyl hydrolase 130 family. http://togogenome.org/gene/300852:TTH_RS04890 ^@ http://purl.uniprot.org/uniprot/Q5SJP6 ^@ Similarity ^@ Belongs to the extradiol ring-cleavage dioxygenase family. http://togogenome.org/gene/300852:TTH_RS03650 ^@ http://purl.uniprot.org/uniprot/Q56425 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the RimP family.|||Cytoplasm|||Required for maturation of 30S ribosomal subunits. http://togogenome.org/gene/300852:TTH_RS06460 ^@ http://purl.uniprot.org/uniprot/P74903 ^@ Function|||Similarity ^@ Belongs to the V-ATPase F subunit family.|||Produces ATP from ADP in the presence of a proton gradient across the membrane. http://togogenome.org/gene/300852:TTH_RS07690 ^@ http://purl.uniprot.org/uniprot/Q5SMH8 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the FGAMS family.|||Cytoplasm|||Monomer. Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.|||Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL. http://togogenome.org/gene/300852:TTH_RS00635 ^@ http://purl.uniprot.org/uniprot/Q5SM23 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ An essential GTPase that binds both GDP and GTP, with rapid nucleotide exchange. Plays a role in 16S rRNA processing and 30S ribosomal subunit biogenesis and possibly also in cell cycle regulation and energy metabolism (By similarity).|||Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Era GTPase family.|||Cell inner membrane|||Cytoplasm|||Monomer (Probable). Binds to the 30S ribosomal subunit, between the head and platform, contacting the 16S rRNA. Contacts ribosomal proteins S7 and S18 and may contact proteins S2 and S11.|||The Era binding site on the 30S subunit overlaps that of protein S1 and is in direct contact with 5 nucleotides directly upstream of the anti-Shine-Dalgarno sequence; the presence of Era may prevent mRNA recruitment. 30S subunits bound to Era are not able to bind to the 50S subunit to make functional ribosomes. Era may serve a role in the final stages of 30S ribosomal subunit assembly. http://togogenome.org/gene/300852:TTH_RS10615 ^@ http://purl.uniprot.org/uniprot/Q53W42 ^@ Similarity ^@ Belongs to the ParB family. http://togogenome.org/gene/300852:TTH_RS00895 ^@ http://purl.uniprot.org/uniprot/P83700 ^@ Function|||Similarity ^@ Belongs to the GHMP kinase family. IspE subfamily.|||Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol. http://togogenome.org/gene/300852:TTH_RS03240 ^@ http://purl.uniprot.org/uniprot/Q5SKM1 ^@ Function|||Similarity ^@ Belongs to the GreA/GreB family.|||Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus. GreA releases sequences of 2 to 3 nucleotides. http://togogenome.org/gene/300852:TTH_RS04995 ^@ http://purl.uniprot.org/uniprot/Q5SJM5 ^@ Similarity ^@ Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily. http://togogenome.org/gene/300852:TTH_RS02385 ^@ http://purl.uniprot.org/uniprot/Q5SL34 ^@ Similarity ^@ Belongs to the CDP-alcohol phosphatidyltransferase class-I family. http://togogenome.org/gene/300852:TTH_RS10205 ^@ http://purl.uniprot.org/uniprot/Q53WB9 ^@ Domain|||Function|||Miscellaneous|||Similarity ^@ Belongs to the CobB/CbiA family.|||Catalyzes the ATP-dependent amidation of the two carboxylate groups at positions a and c of cobyrinate, using either L-glutamine or ammonia as the nitrogen source.|||Comprises of two domains. The C-terminal domain contains the binding site for glutamine and catalyzes the hydrolysis of this substrate to glutamate and ammonia. The N-terminal domain is anticipated to bind ATP and cobyrinate and catalyzes the ultimate synthesis of the diamide product. The ammonia produced via the glutaminase domain is probably translocated to the adjacent domain via a molecular tunnel, where it reacts with an activated intermediate.|||The a and c carboxylates of cobyrinate are activated for nucleophilic attack via formation of a phosphorylated intermediate by ATP. CbiA catalyzes first the amidation of the c-carboxylate, and then that of the a-carboxylate. http://togogenome.org/gene/300852:TTH_RS04305 ^@ http://purl.uniprot.org/uniprot/Q5SK19 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the peptidase M50B family.|||Cell membrane|||Membrane http://togogenome.org/gene/300852:TTH_RS00520 ^@ http://purl.uniprot.org/uniprot/Q5SM43 ^@ Caution|||Function|||PTM|||Similarity|||Subcellular Location Annotation ^@ Belongs to the MurCDEF family. MurE subfamily.|||Carboxylation is probably crucial for Mg(2+) binding and, consequently, for the gamma-phosphate positioning of ATP.|||Catalyzes the addition of an amino acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.|||Cytoplasm|||Lacks conserved residue(s) required for the propagation of feature annotation. http://togogenome.org/gene/300852:TTH_RS08195 ^@ http://purl.uniprot.org/uniprot/Q5SHW1 ^@ Function|||Similarity ^@ Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.|||Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA. http://togogenome.org/gene/300852:TTH_RS11245 ^@ http://purl.uniprot.org/uniprot/Q53WH9 ^@ Function|||Similarity|||Subunit ^@ Belongs to the acetaldehyde dehydrogenase family.|||Catalyzes the conversion of acetaldehyde or propanal to acetyl-CoA or propanoyl-CoA, respectively, using NAD(+) and coenzyme A. The aldehyde substrates can be directly channeled from the aldolase TTHB246 to the dehydrogenase TTHB247. Is the final enzyme in the meta-cleavage pathway for the degradation of aromatic compounds.|||Monomer. Can also form a heterotetramer composed of two aldolase (TTHB246) and two dehydrogenase (TTHB247) subunits. Upon complex formation, the aldolase shows a 5-fold increase in substrate affinity, while the dehydrogenase shows a 3-fold decrease; the kcat values of each enzyme are reduced by 2-fold when they are in a complex. http://togogenome.org/gene/300852:TTH_RS06290 ^@ http://purl.uniprot.org/uniprot/Q5SIX5 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the binding-protein-dependent transport system permease family.|||Cell membrane http://togogenome.org/gene/300852:TTH_RS09000 ^@ http://purl.uniprot.org/uniprot/Q5SHF5 ^@ Function|||Miscellaneous|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the pantothenate synthetase family.|||Catalyzes the condensation of pantoate with beta-alanine in an ATP-dependent reaction via a pantoyl-adenylate intermediate.|||Cytoplasm|||Homodimer.|||PDB 1UFV apparently has a Val-49-Ala mutation.|||The reaction proceeds by a bi uni uni bi ping pong mechanism. http://togogenome.org/gene/300852:TTH_RS01370 ^@ http://purl.uniprot.org/uniprot/Q5SLN5 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the MnmA/TRMU family.|||Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s(2)U34.|||Cytoplasm http://togogenome.org/gene/300852:TTH_RS01635 ^@ http://purl.uniprot.org/uniprot/P98005 ^@ Cofactor|||Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Binds 1 copper B ion per subunit.|||Binds 2 heme groups per subunit.|||Cell membrane|||Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Co I is the catalytic subunit of the enzyme. Electrons originating in cytochrome c are transferred via the copper A center of subunit 2 and heme a of subunit 1 to the bimetallic center formed by heme a3 and copper B. This cytochrome c oxidase shows proton pump activity across the membrane in addition to the electron transfer.|||In the C-terminal section; belongs to the cytochrome c oxidase subunit 3 family.|||In the N-terminal section; belongs to the heme-copper respiratory oxidase family.|||Possibly a heterodimer of A-protein (contains: cytochrome c oxidase subunits I and III) and subunit II. The A-protein could also present a precursor form of subunits I and III. http://togogenome.org/gene/300852:TTH_RS00565 ^@ http://purl.uniprot.org/uniprot/Q5SM35 ^@ Similarity ^@ Belongs to the transketolase family. http://togogenome.org/gene/300852:TTH_RS08435 ^@ http://purl.uniprot.org/uniprot/P80377 ^@ Function|||Similarity|||Subunit ^@ Belongs to the universal ribosomal protein uS13 family.|||Located at the top of the head of the 30S subunit, it contacts several helices of the 16S rRNA. In the 70S ribosome structure it contacts the 23S rRNA (bridge B1a) and protein L5 of the 50S subunit (bridge B1b), connecting the top of the two subunits; these bridges are in contact with the A site and P site tRNAs respectively and are implicated in movement during ribosome translocation. Separately contacts the tRNAs in the A and P sites.|||Part of the 30S ribosomal subunit. Forms a loose heterodimer with protein S19. Forms two bridges to the 50S subunit in the 70S ribosome, contacting protein L5 and the 23S rRNA; these bridges are straddled by the 5S rRNA. http://togogenome.org/gene/300852:TTH_RS03725 ^@ http://purl.uniprot.org/uniprot/Q5SKC6 ^@ Similarity ^@ Belongs to the bacterial solute-binding protein ModA family. http://togogenome.org/gene/300852:TTH_RS08990 ^@ http://purl.uniprot.org/uniprot/Q5SHF7 ^@ Cofactor ^@ Binds 2 Zn(2+) ions per subunit. One is catalytic and the other provides a structural contribution. http://togogenome.org/gene/300852:TTH_RS08455 ^@ http://purl.uniprot.org/uniprot/Q5SHQ9 ^@ Domain|||Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the adenylate kinase family.|||Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism.|||Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon ATP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent ATP hydrolysis.|||Cytoplasm|||Monomer. http://togogenome.org/gene/300852:TTH_RS03260 ^@ http://purl.uniprot.org/uniprot/Q5SKL7 ^@ Function|||Similarity ^@ Belongs to the PEPCase type 1 family.|||Forms oxaloacetate, a four-carbon dicarboxylic acid source for the tricarboxylic acid cycle. http://togogenome.org/gene/300852:TTH_RS01510 ^@ http://purl.uniprot.org/uniprot/Q5SLK7 ^@ Similarity ^@ Belongs to the peptidase S8 family. http://togogenome.org/gene/300852:TTH_RS03265 ^@ http://purl.uniprot.org/uniprot/Q5SKL6 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the MscL family.|||Cell inner membrane|||Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell.|||Homopentamer.|||Membrane http://togogenome.org/gene/300852:TTH_RS00650 ^@ http://purl.uniprot.org/uniprot/Q5SM20 ^@ Similarity ^@ Belongs to the LysR transcriptional regulatory family. http://togogenome.org/gene/300852:TTH_RS08100 ^@ http://purl.uniprot.org/uniprot/Q5SHY0 ^@ Function|||Similarity ^@ Belongs to the RecR family.|||May play a role in DNA repair. It seems to be involved in an RecBC-independent recombinational process of DNA repair. It may act with RecF and RecO. http://togogenome.org/gene/300852:TTH_RS01425 ^@ http://purl.uniprot.org/uniprot/Q5SLM4 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the peptidase A24 family.|||Membrane http://togogenome.org/gene/300852:TTH_RS09360 ^@ http://purl.uniprot.org/uniprot/P05378 ^@ Activity Regulation|||Cofactor|||Function|||Similarity|||Subunit ^@ Belongs to the anthranilate synthase component I family.|||Binds 1 Mg(2+) ion per subunit.|||Feedback inhibited by tryptophan.|||Heterotetramer consisting of two non-identical subunits: a beta subunit (TrpG) and a large alpha subunit (TrpE).|||Part of a heterotetrameric complex that catalyzes the two-step biosynthesis of anthranilate, an intermediate in the biosynthesis of L-tryptophan. In the first step, the glutamine-binding beta subunit (TrpG) of anthranilate synthase (AS) provides the glutamine amidotransferase activity which generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by the large alpha subunit of AS (TrpE) to produce anthranilate. In the absence of TrpG, TrpE can synthesize anthranilate directly from chorismate and high concentrations of ammonia (By similarity). http://togogenome.org/gene/300852:TTH_RS00610 ^@ http://purl.uniprot.org/uniprot/Q5SM27 ^@ Function|||Similarity ^@ Belongs to the BPG-independent phosphoglycerate mutase family. A-PGAM subfamily.|||Catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglycerate. http://togogenome.org/gene/300852:TTH_RS07535 ^@ http://purl.uniprot.org/uniprot/P77527 ^@ Function|||PTM|||Subunit ^@ Forms a heterononamer with DnaK and DnaJ. Three copies of each protein are present in the complex.|||Required for correct assembly of the DnaK-DnaJ complex in the resting state. Seems to stabilize this ternary complex until it is replaced by substrate proteins under heat-shock conditions.|||The N-terminus is blocked. http://togogenome.org/gene/300852:TTH_RS01765 ^@ http://purl.uniprot.org/uniprot/Q5SLF8 ^@ Activity Regulation|||Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the fluoride channel Fluc/FEX (TC 1.A.43) family.|||Cell inner membrane|||Fluoride-specific ion channel. Important for reducing fluoride concentration in the cell, thus reducing its toxicity.|||Na(+) is not transported, but it plays an essential structural role and its presence is essential for fluoride channel function. http://togogenome.org/gene/300852:TTH_RS03010 ^@ http://purl.uniprot.org/uniprot/Q5SKR5 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the uridine kinase family.|||Cytoplasm http://togogenome.org/gene/300852:TTH_RS00040 ^@ http://purl.uniprot.org/uniprot/Q5SMD5 ^@ Similarity ^@ Belongs to the PspA/IM30 family. http://togogenome.org/gene/300852:TTH_RS03995 ^@ http://purl.uniprot.org/uniprot/Q5SK78 ^@ Function|||Induction|||Similarity|||Subcellular Location Annotation|||Subunit ^@ ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins. Required for cellular homeostasis and for survival from DNA damage and developmental changes induced by stress. Degrades polypeptides processively to yield small peptide fragments that are 5 to 10 amino acids long. Binds to DNA in a double-stranded, site-specific manner.|||Belongs to the peptidase S16 family.|||By heat shock.|||Cytoplasm|||Homohexamer. Organized in a ring with a central cavity. http://togogenome.org/gene/300852:TTH_RS06310 ^@ http://purl.uniprot.org/uniprot/Q5SIX1 ^@ Function|||Similarity ^@ ATPase required for the correct placement of the division site. Cell division inhibitors MinC and MinD act in concert to form an inhibitor capable of blocking formation of the polar Z ring septums. Rapidly oscillates between the poles of the cell to destabilize FtsZ filaments that have formed before they mature into polar Z rings.|||Belongs to the ParA family. MinD subfamily. http://togogenome.org/gene/300852:TTH_RS01860 ^@ http://purl.uniprot.org/uniprot/Q5SLE0 ^@ Similarity ^@ Belongs to the phosphohexose mutase family. http://togogenome.org/gene/300852:TTH_RS07560 ^@ http://purl.uniprot.org/uniprot/Q5SI81 ^@ Disruption Phenotype|||Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the methyltransferase superfamily. RlmI family.|||Cytoplasm|||Lack of methylation of cytosine at position 1942 (m5C1942) of 23S rRNA.|||Monomer (in vitro).|||Specifically methylates the cytosine at position 1942 (m5C1942) of 23S rRNA. http://togogenome.org/gene/300852:TTH_RS10525 ^@ http://purl.uniprot.org/uniprot/Q53W55 ^@ Function|||Similarity ^@ Antitoxin component of a type II toxin-antitoxin (TA) system.|||Belongs to the phD/YefM antitoxin family. http://togogenome.org/gene/300852:TTH_RS06035 ^@ http://purl.uniprot.org/uniprot/Q5SJ26 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the Maf family. YhdE subfamily.|||Cytoplasm|||Nucleoside triphosphate pyrophosphatase that hydrolyzes dTTP and UTP. May have a dual role in cell division arrest and in preventing the incorporation of modified nucleotides into cellular nucleic acids. http://togogenome.org/gene/300852:TTH_RS07420 ^@ http://purl.uniprot.org/uniprot/Q5SIA8 ^@ Activity Regulation|||Function|||Miscellaneous|||Similarity|||Subunit ^@ Allosterically activated by GTP, when glutamine is the substrate (PubMed:15296735). GTP has no effect on the reaction when ammonia is the substrate. The allosteric effector GTP functions by stabilizing the protein conformation that binds the tetrahedral intermediate(s) formed during glutamine hydrolysis. Inhibited by the product CTP, via allosteric rather than competitive inhibition (By similarity).|||Belongs to the CTP synthase family.|||CTPSs have evolved a hybrid strategy for distinguishing between UTP and CTP. The overlapping regions of the product feedback inhibitory and substrate sites recognize a common feature in both compounds, the triphosphate moiety. To differentiate isosteric substrate and product pyrimidine rings, an additional pocket far from the expected kinase/ligase catalytic site, specifically recognizes the cytosine and ribose portions of the product inhibitor.|||Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen (PubMed:15296735). Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates (By similarity).|||Homotetramer in the presence of UTP and ATP. Is in a protein concentration-dependent equilibrium between monomer, dimer, and tetramer in the absence of UTP and ATP. http://togogenome.org/gene/300852:TTH_RS03515 ^@ http://purl.uniprot.org/uniprot/Q5SKG9 ^@ Cofactor|||Function|||Similarity ^@ Belongs to the thiamine-phosphate synthase family.|||Binds 1 Mg(2+) ion per subunit.|||Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP). http://togogenome.org/gene/300852:TTH_RS02380 ^@ http://purl.uniprot.org/uniprot/Q5SL35 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the cytidylate kinase family. Type 1 subfamily.|||Cytoplasm http://togogenome.org/gene/300852:TTH_RS02900 ^@ http://purl.uniprot.org/uniprot/Q5SKT7 ^@ Activity Regulation|||Function|||Similarity|||Subunit ^@ Belongs to the PNP/UDP phosphorylase family. Futalosine hydrolase subfamily.|||Catalyzes the hydrolysis of futalosine (FL) to dehypoxanthine futalosine (DHFL) and hypoxanthine, a step in the biosynthesis of menaquinone (MK, vitamin K2). Is highly specific to futalosine since it does not accept aminodeoxyfutalosine (AFL), or any structurally related nucleotides and nucleosides as substrate.|||Homotetramer.|||No enhancing of inhibitory effects are observed with divalent metal ions. Slightly inhibited by hypoxanthine. http://togogenome.org/gene/300852:TTH_RS04395 ^@ http://purl.uniprot.org/uniprot/Q5SK01 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.|||Cytoplasm|||Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA. http://togogenome.org/gene/300852:TTH_RS01035 ^@ http://purl.uniprot.org/uniprot/Q5SLU5 ^@ Cofactor|||Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the RNase HII family.|||Cytoplasm|||Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.|||Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding. http://togogenome.org/gene/300852:TTH_RS08465 ^@ http://purl.uniprot.org/uniprot/Q5SHQ7 ^@ Function|||Similarity|||Subunit ^@ Belongs to the universal ribosomal protein uL15 family.|||Binds to the 23S rRNA.|||Part of the 50S ribosomal subunit. http://togogenome.org/gene/300852:TTH_RS00285 ^@ http://purl.uniprot.org/uniprot/Q5SM89 ^@ Caution|||Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family. TrmL subfamily.|||Could methylate the ribose at the nucleotide 34 wobble position in tRNA.|||Cytoplasm|||Lacks conserved residue(s) required for the propagation of feature annotation. http://togogenome.org/gene/300852:TTH_RS02375 ^@ http://purl.uniprot.org/uniprot/Q5SL36 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the EPSP synthase family.|||Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate.|||Cytoplasm|||Monomer. http://togogenome.org/gene/300852:TTH_RS10875 ^@ http://purl.uniprot.org/uniprot/Q53VZ6 ^@ Subcellular Location Annotation ^@ Cytoplasm http://togogenome.org/gene/300852:TTH_RS09860 ^@ http://purl.uniprot.org/uniprot/Q5SGY9 ^@ Subcellular Location Annotation ^@ Membrane http://togogenome.org/gene/300852:TTH_RS03490 ^@ http://purl.uniprot.org/uniprot/Q5SKH4 ^@ Cofactor|||Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the PAPS reductase family. CysH subfamily.|||Binds 1 [4Fe-4S] cluster per subunit.|||Catalyzes the formation of sulfite from adenosine 5'-phosphosulfate (APS) using thioredoxin as an electron donor.|||Cytoplasm http://togogenome.org/gene/300852:TTH_RS06950 ^@ http://purl.uniprot.org/uniprot/Q5SIJ5 ^@ Cofactor|||Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ A monovalent cation. Ammonium or potassium.|||Belongs to the type III pantothenate kinase family.|||Catalyzes the phosphorylation of pantothenate (Pan), the first step in CoA biosynthesis.|||Cytoplasm|||Homodimer. http://togogenome.org/gene/300852:TTH_RS07540 ^@ http://purl.uniprot.org/uniprot/Q56237 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the DnaJ family.|||Cytoplasm|||Does not influence ATP binding or hydrolysis nor ADP release. Exerts influence on the interaction of DnaK with substrates; in the presence of DafA, DnaJ inhibits substrate binding, and substrate already bound to DnaK is displaced by DnaJ and DafA.|||Forms a heterononamer with DnaJ and DafA in the resting state. Three copies of each protein are present in the complex. http://togogenome.org/gene/300852:TTH_RS02960 ^@ http://purl.uniprot.org/uniprot/Q5SKS5 ^@ Subcellular Location Annotation ^@ Membrane http://togogenome.org/gene/300852:TTH_RS06450 ^@ http://purl.uniprot.org/uniprot/Q56404 ^@ Function|||Similarity ^@ Belongs to the ATPase alpha/beta chains family.|||Produces ATP from ADP in the presence of a proton gradient across the membrane. The V-type beta chain is a regulatory subunit. http://togogenome.org/gene/300852:TTH_RS00130 ^@ http://purl.uniprot.org/uniprot/Q5SMC0 ^@ Similarity ^@ Belongs to the peptidase S16 family. http://togogenome.org/gene/300852:TTH_RS02490 ^@ http://purl.uniprot.org/uniprot/Q5SL13 ^@ Similarity ^@ Belongs to the glycosyltransferase 20 family. http://togogenome.org/gene/300852:TTH_RS09995 ^@ http://purl.uniprot.org/uniprot/Q5SGW1 ^@ Similarity ^@ Belongs to the ParB family. http://togogenome.org/gene/300852:TTH_RS08015 ^@ http://purl.uniprot.org/uniprot/P29749 ^@ Function|||Similarity ^@ A gamma subtype methylase, recognizes the double-stranded sequence 5'-TCGA-3', methylates A-4 on both strands and protects the DNA from cleavage by the TthHB8I endonuclease.|||Belongs to the N(4)/N(6)-methyltransferase family. http://togogenome.org/gene/300852:TTH_RS04825 ^@ http://purl.uniprot.org/uniprot/Q5SJQ9 ^@ Domain|||Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ A Gly-cisPro motif from one monomer fits into the active site of the other monomer to allow specific chiral rejection of L-amino acids.|||An aminoacyl-tRNA editing enzyme that deacylates mischarged D-aminoacyl-tRNAs. Also deacylates mischarged glycyl-tRNA(Ala), protecting cells against glycine mischarging by AlaRS. Acts via tRNA-based rather than protein-based catalysis; rejects L-amino acids rather than detecting D-amino acids in the active site. By recycling D-aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl-tRNA entities in vivo and helps enforce protein L-homochirality.|||Belongs to the DTD family.|||Cytoplasm|||Homodimer. http://togogenome.org/gene/300852:TTH_RS01870 ^@ http://purl.uniprot.org/uniprot/Q5SLD8 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the binding-protein-dependent transport system permease family.|||Cell membrane http://togogenome.org/gene/300852:TTH_RS02125 ^@ http://purl.uniprot.org/uniprot/Q5SL86 ^@ Cofactor|||Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the PanB family.|||Binds 1 Mg(2+) ion per subunit.|||Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is transferred onto alpha-ketoisovalerate to form ketopantoate.|||Cytoplasm|||Homodecamer; pentamer of dimers. http://togogenome.org/gene/300852:TTH_RS02930 ^@ http://purl.uniprot.org/uniprot/Q5SKT1 ^@ Function|||Similarity ^@ Belongs to the PNP/MTAP phosphorylase family.|||The purine nucleoside phosphorylases catalyze the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate. http://togogenome.org/gene/300852:TTH_RS06465 ^@ http://purl.uniprot.org/uniprot/P74902 ^@ Function|||Similarity ^@ Belongs to the V-ATPase V0D/AC39 subunit family.|||Produces ATP from ADP in the presence of a proton gradient across the membrane. http://togogenome.org/gene/300852:TTH_RS10240 ^@ http://purl.uniprot.org/uniprot/Q53WB2 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the NiCoT transporter (TC 2.A.52) family.|||Cell membrane|||Membrane http://togogenome.org/gene/300852:TTH_RS04880 ^@ http://purl.uniprot.org/uniprot/Q5SJP8 ^@ Function|||Similarity|||Subunit ^@ Belongs to the FADH(2)-utilizing monooxygenase family.|||Homotetramer consisting of a dimer of dimers. 4-HPA 3-monooxygenase consists of a reductase component HpaC and an oxygenase component HpaB.|||Utilizes FADH(2) supplied by HpaC, to catalyze the hydroxylation of 4-hydroxyphenylacetic acid, leading to the production of 3,4-dihydroxyphenylacetic acid (DHPA). http://togogenome.org/gene/300852:TTH_RS01300 ^@ http://purl.uniprot.org/uniprot/P35872 ^@ Function|||Similarity ^@ Belongs to the NusG family.|||Participates in transcription elongation, termination and antitermination. http://togogenome.org/gene/300852:TTH_RS05305 ^@ http://purl.uniprot.org/uniprot/Q5SJG6 ^@ Domain|||Function|||Similarity|||Subunit ^@ Belongs to the GreA/GreB family.|||Inhibitory activity is regulated via a pH-induced conformational change of the structure. At pH above 7, Gfh1 is in an inactive flipped orientation that prevents binding to RNAP. At lower pH, Gfh1 switches to an active orientation, which enables binding to RNAP and inhibitory activity (By similarity).|||Inhibits all catalytic activities of RNA polymerase (RNAP) by partially occluding its substrate-binding site and preventing NTP binding.|||Interacts with RNAP. http://togogenome.org/gene/300852:TTH_RS04265 ^@ http://purl.uniprot.org/uniprot/Q5SK26 ^@ Similarity ^@ Belongs to the FtsK/SpoIIIE/SftA family. http://togogenome.org/gene/300852:TTH_RS00995 ^@ http://purl.uniprot.org/uniprot/Q5SLV3 ^@ Function|||Similarity ^@ Belongs to the DHNA family.|||Catalyzes the conversion of 7,8-dihydroneopterin to 6-hydroxymethyl-7,8-dihydropterin. http://togogenome.org/gene/300852:TTH_RS02230 ^@ http://purl.uniprot.org/uniprot/Q5SL65 ^@ Similarity|||Subunit ^@ Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. Histidinol-phosphate aminotransferase subfamily.|||Homodimer. http://togogenome.org/gene/300852:TTH_RS05615 ^@ http://purl.uniprot.org/uniprot/Q5SJB1 ^@ Similarity ^@ Belongs to the FemABX family. http://togogenome.org/gene/300852:TTH_RS00015 ^@ http://purl.uniprot.org/uniprot/Q5SME0 ^@ Similarity ^@ Belongs to the pyruvate kinase family. http://togogenome.org/gene/300852:TTH_RS08475 ^@ http://purl.uniprot.org/uniprot/Q5SHQ5 ^@ Domain|||Function|||Miscellaneous|||Similarity|||Subunit ^@ Belongs to the universal ribosomal protein uS5 family.|||Is positioned such that it could physically interact with spectinomycin.|||Located at the back of the 30S subunit body where it stabilizes the conformation of the head with respect to the body. Binds mRNA in the 70S ribosome, positioning it for translation.|||Part of the 30S ribosomal subunit. Contacts proteins S4 and S8.|||The N-terminal domain interacts with the head of the 30S subunit; the C-terminal domain interacts with the body and contacts protein S4. The interaction surface between S4 and S5 is involved in control of translational fidelity.|||With S4 and S12 plays an important role in translational accuracy. http://togogenome.org/gene/300852:TTH_RS02555 ^@ http://purl.uniprot.org/uniprot/Q5SL02 ^@ Function|||Similarity ^@ Belongs to the threonine synthase family.|||Catalyzes the gamma-elimination of phosphate from L-phosphohomoserine and the beta-addition of water to produce L-threonine. http://togogenome.org/gene/300852:TTH_RS04730 ^@ http://purl.uniprot.org/uniprot/Q5SJS9 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the bacterial CoaD family.|||Cytoplasm|||Homohexamer. All subunits in the hexamer bind substrate. Is in equilibrium between two trimers and a hexamer, and the trimer may be a physiologically important form in T.thermophilus.|||Reversibly transfers an adenylyl group from ATP to 4'-phosphopantetheine, yielding dephospho-CoA (dPCoA) and pyrophosphate. http://togogenome.org/gene/300852:TTH_RS09905 ^@ http://purl.uniprot.org/uniprot/Q5SGY0 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the acetylglutamate kinase family. ArgB subfamily.|||Catalyzes the ATP-dependent phosphorylation of N-acetyl-L-glutamate.|||Cytoplasm http://togogenome.org/gene/300852:TTH_RS06445 ^@ http://purl.uniprot.org/uniprot/O87880 ^@ Function|||Similarity ^@ Belongs to the V-ATPase D subunit family.|||Produces ATP from ADP in the presence of a proton gradient across the membrane. http://togogenome.org/gene/300852:TTH_RS05710 ^@ http://purl.uniprot.org/uniprot/Q5SJ91 ^@ Function|||Subunit ^@ Acetyl-CoA carboxylase is a heterohexamer of biotin carboxyl carrier protein, biotin carboxylase and the two subunits of carboxyl transferase in a 2:2 complex.|||This protein is a component of the acetyl coenzyme A carboxylase complex; first, biotin carboxylase catalyzes the carboxylation of the carrier protein and then the transcarboxylase transfers the carboxyl group to form malonyl-CoA. http://togogenome.org/gene/300852:TTH_RS00385 ^@ http://purl.uniprot.org/uniprot/Q5SM69 ^@ Caution|||Function|||Similarity|||Subunit ^@ Belongs to the type IA topoisomerase family.|||Lacks conserved residue(s) required for the propagation of feature annotation.|||Monomer.|||Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. http://togogenome.org/gene/300852:TTH_RS02845 ^@ http://purl.uniprot.org/uniprot/Q5SKU8 ^@ Caution|||Function|||Similarity|||Subunit ^@ Belongs to the aspartate-semialdehyde dehydrogenase family.|||Catalyzes the NADPH-dependent formation of L-aspartate-semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl-4-phosphate.|||Homodimer.|||Lacks conserved residue(s) required for the propagation of feature annotation. http://togogenome.org/gene/300852:TTH_RS05630 ^@ http://purl.uniprot.org/uniprot/Q5SJA8 ^@ Subcellular Location Annotation ^@ Membrane http://togogenome.org/gene/300852:TTH_RS09350 ^@ http://purl.uniprot.org/uniprot/Q5SH88 ^@ Cofactor|||Function|||Similarity|||Subunit ^@ Belongs to the anthranilate phosphoribosyltransferase family.|||Binds 2 magnesium ions per monomer.|||Catalyzes the transfer of the phosphoribosyl group of 5-phosphorylribose-1-pyrophosphate (PRPP) to anthranilate to yield N-(5'-phosphoribosyl)-anthranilate (PRA).|||Homodimer. http://togogenome.org/gene/300852:TTH_RS06230 ^@ http://purl.uniprot.org/uniprot/Q5SIY7 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the class-I aminoacyl-tRNA synthetase family.|||Catalyzes the attachment of tryptophan to tRNA(Trp).|||Cytoplasm|||Homodimer. http://togogenome.org/gene/300852:TTH_RS07860 ^@ http://purl.uniprot.org/uniprot/Q5SI28 ^@ Function|||Subunit ^@ Catalyzes the synthesis of GMP from XMP.|||Homodimer. http://togogenome.org/gene/300852:TTH_RS07010 ^@ http://purl.uniprot.org/uniprot/Q5SII5 ^@ Cofactor|||Function|||Similarity|||Subunit ^@ Belongs to the chorismate synthase family.|||Catalyzes the anti-1,4-elimination of the C-3 phosphate and the C-6 proR hydrogen from 5-enolpyruvylshikimate-3-phosphate (EPSP) to yield chorismate, which is the branch point compound that serves as the starting substrate for the three terminal pathways of aromatic amino acid biosynthesis. This reaction introduces a second double bond into the aromatic ring system.|||Homotetramer.|||Reduced FMN (FMNH(2)). http://togogenome.org/gene/300852:TTH_RS10420 ^@ http://purl.uniprot.org/uniprot/Q53W76 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the binding-protein-dependent transport system permease family.|||Cell membrane http://togogenome.org/gene/300852:TTH_RS05995 ^@ http://purl.uniprot.org/uniprot/Q5SJ32 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family.|||Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA.|||Cytoplasm|||Homodimer. http://togogenome.org/gene/300852:TTH_RS05755 ^@ http://purl.uniprot.org/uniprot/P82543 ^@ Subcellular Location Annotation ^@ Cell membrane http://togogenome.org/gene/300852:TTH_RS01815 ^@ http://purl.uniprot.org/uniprot/Q5SLE8 ^@ Cofactor|||Function|||Miscellaneous|||Similarity|||Subunit ^@ Belongs to the HMBS family.|||Binds 1 dipyrromethane group covalently.|||Monomer.|||Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps.|||The porphobilinogen subunits are added to the dipyrromethane group. http://togogenome.org/gene/300852:TTH_RS10230 ^@ http://purl.uniprot.org/uniprot/Q7SIC7 ^@ Function|||Similarity ^@ Belongs to the CobT family.|||Catalyzes the synthesis of alpha-ribazole-5'-phosphate from nicotinate mononucleotide (NAMN) and 5,6-dimethylbenzimidazole (DMB). http://togogenome.org/gene/300852:TTH_RS10265 ^@ http://purl.uniprot.org/uniprot/Q53WA7 ^@ Similarity ^@ Belongs to the precorrin methyltransferase family. http://togogenome.org/gene/300852:TTH_RS02030 ^@ http://purl.uniprot.org/uniprot/Q5SLA6 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the TatC family.|||Cell inner membrane|||Forms a complex with TatA.|||Membrane|||Part of the twin-arginine translocation (Tat) system that transports large folded proteins containing a characteristic twin-arginine motif in their signal peptide across membranes. http://togogenome.org/gene/300852:TTH_RS09160 ^@ http://purl.uniprot.org/uniprot/Q5SHC6 ^@ Similarity ^@ Belongs to the acyl coenzyme A hydrolase family. http://togogenome.org/gene/300852:TTH_RS07230 ^@ http://purl.uniprot.org/uniprot/Q5SIE6 ^@ Similarity ^@ Belongs to the phosphohexose mutase family. http://togogenome.org/gene/300852:TTH_RS08845 ^@ http://purl.uniprot.org/uniprot/Q5SHI5 ^@ Similarity ^@ Belongs to the archease family. http://togogenome.org/gene/300852:TTH_RS04425 ^@ http://purl.uniprot.org/uniprot/Q5SJZ5 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the CDS family.|||Membrane http://togogenome.org/gene/300852:TTH_RS01865 ^@ http://purl.uniprot.org/uniprot/Q5SLD9 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the binding-protein-dependent transport system permease family.|||Cell membrane http://togogenome.org/gene/300852:TTH_RS02270 ^@ http://purl.uniprot.org/uniprot/Q5SL57 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the GTP-binding SRP family. FtsY subfamily.|||Cell inner membrane|||Cytoplasm|||Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Acts as a receptor for the complex formed by the signal recognition particle (SRP) and the ribosome-nascent chain (RNC).|||Membrane|||Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY. http://togogenome.org/gene/300852:TTH_RS08450 ^@ http://purl.uniprot.org/uniprot/Q5SHR0 ^@ Cofactor|||Function|||Similarity|||Subunit ^@ Belongs to the peptidase M24A family. Methionine aminopeptidase type 1 subfamily.|||Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe(2+)-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.|||Monomer.|||Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Requires deformylation of the N(alpha)-formylated initiator methionine before it can be hydrolyzed. http://togogenome.org/gene/300852:TTH_RS06305 ^@ http://purl.uniprot.org/uniprot/Q5SIX2 ^@ Function|||Similarity ^@ Belongs to the MinE family.|||Prevents the cell division inhibition by proteins MinC and MinD at internal division sites while permitting inhibition at polar sites. This ensures cell division at the proper site by restricting the formation of a division septum at the midpoint of the long axis of the cell. http://togogenome.org/gene/300852:TTH_RS03085 ^@ http://purl.uniprot.org/uniprot/Q5SKP9 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the bacterial solute-binding protein 7 family.|||Homodimer.|||Part of the tripartite ATP-independent periplasmic (TRAP) transport system.|||Periplasm http://togogenome.org/gene/300852:TTH_RS00230 ^@ http://purl.uniprot.org/uniprot/Q5SMA0 ^@ Caution|||Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the peptidase A8 family.|||Cell inner membrane|||Lacks conserved residue(s) required for the propagation of feature annotation.|||This protein specifically catalyzes the removal of signal peptides from prolipoproteins. http://togogenome.org/gene/300852:TTH_RS07840 ^@ http://purl.uniprot.org/uniprot/Q5SI32 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the UvrC family.|||Cytoplasm|||Interacts with UvrB in an incision complex.|||The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrC both incises the 5' and 3' sides of the lesion. The N-terminal half is responsible for the 3' incision and the C-terminal half is responsible for the 5' incision. http://togogenome.org/gene/300852:TTH_RS09220 ^@ http://purl.uniprot.org/uniprot/Q5SHB4 ^@ Caution|||Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the FtsA/MreB family.|||Cytoplasm|||Forms membrane-associated dynamic filaments that are essential for cell shape determination. Acts by regulating cell wall synthesis and cell elongation, and thus cell shape. A feedback loop between cell geometry and MreB localization may maintain elongated cell shape by targeting cell wall growth to regions of negative cell wall curvature.|||Forms polymers.|||Lacks conserved residue(s) required for the propagation of feature annotation. http://togogenome.org/gene/300852:TTH_RS08630 ^@ http://purl.uniprot.org/uniprot/Q5SHM4 ^@ Subcellular Location Annotation ^@ Membrane http://togogenome.org/gene/300852:TTH_RS08570 ^@ http://purl.uniprot.org/uniprot/Q5SHN6 ^@ Function|||PTM|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.|||Cytoplasm|||EF-Tu-GDP binds to the acceptor arm of tmRNA by interacting with its acceptor arm, suggesting that GTP hydrolysis by EF-Tu is essential for tmRNA function.|||Monomer (By similarity). Binds to the 70S ribosome, contacts tmRNA during trans-translation (PubMed:12677067).|||Phosphorylated on a threonine.|||Protects glycyl-tRNA(Gly) from hydrolysis by E.coli D-aminoacyl-tRNA deacylase (dtd) (PubMed:27224426).|||This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. http://togogenome.org/gene/300852:TTH_RS05425 ^@ http://purl.uniprot.org/uniprot/Q5SJE8 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the transaldolase family. Type 3B subfamily.|||Cytoplasm|||Transaldolase is important for the balance of metabolites in the pentose-phosphate pathway. http://togogenome.org/gene/300852:TTH_RS03970 ^@ http://purl.uniprot.org/uniprot/Q5SK83 ^@ Similarity ^@ Belongs to the peptidase S58 family. http://togogenome.org/gene/300852:TTH_RS03220 ^@ http://purl.uniprot.org/uniprot/Q5SKM5 ^@ Domain|||Function|||Similarity ^@ Belongs to the vitamin-B12 dependent methionine synthase family.|||Catalyzes the transfer of a methyl group from methyl-cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate.|||Modular enzyme with four functionally distinct domains. The isolated Hcy-binding domain catalyzes methyl transfer from free methylcobalamin to homocysteine. The Hcy-binding domain in association with the pterin-binding domain catalyzes the methylation of cob(I)alamin by methyltetrahydrofolate and the methylation of homocysteine. The B12-binding domain binds the cofactor. The AdoMet activation domain binds S-adenosyl-L-methionine. Under aerobic conditions cob(I)alamin can be converted to inactive cob(II)alamin. Reductive methylation by S-adenosyl-L-methionine and flavodoxin regenerates methylcobalamin. http://togogenome.org/gene/300852:TTH_RS04240 ^@ http://purl.uniprot.org/uniprot/Q5SK31 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the TACO1 family.|||Cytoplasm http://togogenome.org/gene/300852:TTH_RS01605 ^@ http://purl.uniprot.org/uniprot/Q5SLI8 ^@ Cofactor|||Function|||Similarity ^@ Belongs to the IspG family.|||Binds 1 [4Fe-4S] cluster.|||Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate. http://togogenome.org/gene/300852:TTH_RS08315 ^@ http://purl.uniprot.org/uniprot/Q5SHT7 ^@ Function|||Similarity ^@ Belongs to the aspartate/glutamate racemases family.|||Provides the (R)-glutamate required for cell wall biosynthesis. http://togogenome.org/gene/300852:TTH_RS02135 ^@ http://purl.uniprot.org/uniprot/Q5SL84 ^@ Similarity ^@ Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily. http://togogenome.org/gene/300852:TTH_RS03115 ^@ http://purl.uniprot.org/uniprot/Q5SKP3 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the ferrochelatase family.|||Catalyzes the ferrous insertion into protoporphyrin IX.|||Cytoplasm http://togogenome.org/gene/300852:TTH_RS04605 ^@ http://purl.uniprot.org/uniprot/Q5SJV9 ^@ Cofactor|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the class-I aminoacyl-tRNA synthetase family.|||Binds 1 zinc ion per subunit.|||Cytoplasm|||Monomer. http://togogenome.org/gene/300852:TTH_RS04510 ^@ http://purl.uniprot.org/uniprot/Q5SJX8 ^@ Cofactor|||Function|||Similarity|||Subunit ^@ Acts as a N-succinylamino acid racemase (NSAR) that catalyzes the racemization of N-succinyl-L-phenylglycine (PubMed:24872444). Also converts 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate (SHCHC) to 2-succinylbenzoate (OSB) (PubMed:24872444). Catalyzes both N-succinylamino acid racemization and OSB synthesis at equivalent rates (PubMed:24872444). However, NSAR activity is probably the protein's biological function, because menaquinone biosynthesis genes are missing in this species (Probable).|||Belongs to the mandelate racemase/muconate lactonizing enzyme family. MenC type 2 subfamily.|||Binds 1 divalent metal cation per subunit.|||Homooctamer. Tetramer of dimers. http://togogenome.org/gene/300852:TTH_RS01255 ^@ http://purl.uniprot.org/uniprot/Q5SLQ3 ^@ Function|||Miscellaneous|||Similarity|||Subcellular Location Annotation ^@ Belongs to the LipB family.|||Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate.|||Cytoplasm|||In the reaction, the free carboxyl group of octanoic acid is attached via an amide linkage to the epsilon-amino group of a specific lysine residue of lipoyl domains of lipoate-dependent enzymes. http://togogenome.org/gene/300852:TTH_RS09040 ^@ http://purl.uniprot.org/uniprot/P60492 ^@ Function|||Similarity|||Subunit ^@ Belongs to the bacterial ribosomal protein bL21 family.|||Contacts protein L20 (By similarity). Part of the 50S ribosomal subunit.|||This protein binds to 23S rRNA in the presence of protein L20 (By similarity). Found on the solvent side of the large subunit. http://togogenome.org/gene/300852:TTH_RS01440 ^@ http://purl.uniprot.org/uniprot/P61493 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the GroES chaperonin family.|||Cytoplasm|||Heptamer of 7 subunits arranged in a ring. Interacts with the chaperonin GroEL.|||Together with the chaperonin GroEL, plays an essential role in assisting protein folding. The GroEL-GroES system forms a nano-cage that allows encapsulation of the non-native substrate proteins and provides a physical environment optimized to promote and accelerate protein folding. GroES binds to the apical surface of the GroEL ring, thereby capping the opening of the GroEL channel. http://togogenome.org/gene/300852:TTH_RS08310 ^@ http://purl.uniprot.org/uniprot/Q5SHT8 ^@ Cofactor|||Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the AdoMet synthase family.|||Binds 1 potassium ion per subunit.|||Binds 2 divalent ions per subunit.|||Catalyzes the formation of S-adenosylmethionine (AdoMet) from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme.|||Cytoplasm|||Homotetramer; dimer of dimers. http://togogenome.org/gene/300852:TTH_RS04435 ^@ http://purl.uniprot.org/uniprot/P43891 ^@ Activity Regulation|||Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the UMP kinase family.|||Catalyzes the reversible phosphorylation of UMP to UDP.|||Cytoplasm|||Homohexamer.|||Inhibited by UTP. http://togogenome.org/gene/300852:TTH_RS02210 ^@ http://purl.uniprot.org/uniprot/Q5SL69 ^@ Caution|||Function|||Miscellaneous|||Similarity ^@ Belongs to the thiamine-monophosphate kinase family.|||Catalyzes the ATP-dependent phosphorylation of thiamine-monophosphate (TMP) to form thiamine-pyrophosphate (TPP), the active form of vitamin B1.|||Lacks conserved residue(s) required for the propagation of feature annotation.|||Reaction mechanism of ThiL seems to utilize a direct, inline transfer of the gamma-phosphate of ATP to TMP rather than a phosphorylated enzyme intermediate. http://togogenome.org/gene/300852:TTH_RS00490 ^@ http://purl.uniprot.org/uniprot/Q56226 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the complex I subunit 4L family.|||Cell inner membrane|||NDH-1 is composed of 15 different subunits, Nqo1 to Nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits Nqo7, Nqo8 and Nqo10 to Nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits Nqo1 to Nqo6, Nqo9 and Nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers.|||NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP. http://togogenome.org/gene/300852:TTH_RS05855 ^@ http://purl.uniprot.org/uniprot/Q5SJ61 ^@ Cofactor|||Function|||Similarity|||Subunit ^@ Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.|||Binds 1 FAD per subunit.|||Homodimer.|||Resistance to Hg(2+) in bacteria appears to be governed by a specialized system which includes mercuric reductase. MerA protein is responsible for volatilizing mercury as Hg(0). http://togogenome.org/gene/300852:TTH_RS05510 ^@ http://purl.uniprot.org/uniprot/Q5SJD2 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the MurCDEF family.|||Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA).|||Cytoplasm http://togogenome.org/gene/300852:TTH_RS07125 ^@ http://purl.uniprot.org/uniprot/Q5SIG2 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the CcmB/CycW/HelB family.|||Membrane http://togogenome.org/gene/300852:TTH_RS01995 ^@ http://purl.uniprot.org/uniprot/Q5SLB3 ^@ Similarity ^@ Belongs to the AOR/FOR family. http://togogenome.org/gene/300852:TTH_RS02815 ^@ http://purl.uniprot.org/uniprot/Q5SKV3 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the PINc/VapC protein family.|||Membrane|||Part of a toxin-antitoxin (TA) system. An RNase. http://togogenome.org/gene/300852:TTH_RS06395 ^@ http://purl.uniprot.org/uniprot/Q5SIV3 ^@ Similarity ^@ Belongs to the disproportionating enzyme family. http://togogenome.org/gene/300852:TTH_RS02280 ^@ http://purl.uniprot.org/uniprot/P27000 ^@ Activity Regulation|||Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.|||Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).|||Cytoplasm|||In the absence of bound tRNA, ATP is bound in a non-productive mode, and the enzyme cannot activate amino acids.|||Monomer. http://togogenome.org/gene/300852:TTH_RS10975 ^@ http://purl.uniprot.org/uniprot/Q53WG8 ^@ Function|||Similarity|||Subunit ^@ Belongs to the CRISPR-associated endonuclease Cas1 family.|||CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Acts as a dsDNA endonuclease. Involved in the integration of spacer DNA into the CRISPR cassette.|||Homodimer, forms a heterotetramer with a Cas2 homodimer. http://togogenome.org/gene/300852:TTH_RS06645 ^@ http://purl.uniprot.org/uniprot/Q5SIQ7 ^@ Activity Regulation|||Cofactor|||Function|||Similarity ^@ Allosterically activated by GTP.|||Belongs to the UPRTase family.|||Binds 1 Mg(2+) ion per subunit. The magnesium is bound as Mg-PRPP.|||Catalyzes the conversion of uracil and 5-phospho-alpha-D-ribose 1-diphosphate (PRPP) to UMP and diphosphate. http://togogenome.org/gene/300852:TTH_RS03940 ^@ http://purl.uniprot.org/uniprot/Q5SK89 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the AB hydrolase superfamily. MetX family.|||Cytoplasm|||Homodimer.|||Transfers an acetyl group from acetyl-CoA to L-homoserine, forming acetyl-L-homoserine. http://togogenome.org/gene/300852:TTH_RS02880 ^@ http://purl.uniprot.org/uniprot/Q5SKU1 ^@ Similarity|||Subunit ^@ Belongs to the bacterial ribosomal protein bL35 family.|||Part of the 50S ribosomal subunit. http://togogenome.org/gene/300852:TTH_RS01135 ^@ http://purl.uniprot.org/uniprot/Q5SLS5 ^@ Similarity ^@ Belongs to the enoyl-CoA hydratase/isomerase family. http://togogenome.org/gene/300852:TTH_RS05650 ^@ http://purl.uniprot.org/uniprot/Q5SJA3 ^@ Function|||Induction|||Similarity|||Subcellular Location Annotation|||Subunit ^@ ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins. Required for cellular homeostasis and for survival from DNA damage and developmental changes induced by stress. Degrades polypeptides processively to yield small peptide fragments that are 5 to 10 amino acids long. Binds to DNA in a double-stranded, site-specific manner.|||Belongs to the peptidase S16 family.|||By heat shock.|||Cytoplasm|||Homohexamer. Organized in a ring with a central cavity. http://togogenome.org/gene/300852:TTH_RS03110 ^@ http://purl.uniprot.org/uniprot/Q5SKP4 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the protoporphyrinogen/coproporphyrinogen oxidase family. Coproporphyrinogen III oxidase subfamily.|||Cytoplasm|||Involved in coproporphyrin-dependent heme b biosynthesis. Catalyzes the oxidation of coproporphyrinogen III to coproporphyrin III. http://togogenome.org/gene/300852:TTH_RS06470 ^@ http://purl.uniprot.org/uniprot/P74901 ^@ Function|||Similarity ^@ Belongs to the V-ATPase E subunit family.|||Produces ATP from ADP in the presence of a proton gradient across the membrane. http://togogenome.org/gene/300852:TTH_RS10675 ^@ http://purl.uniprot.org/uniprot/Q53W31 ^@ Function|||Similarity ^@ Belongs to the PINc/VapC protein family.|||Toxic component of a toxin-antitoxin (TA) system. An RNase. http://togogenome.org/gene/300852:TTH_RS05270 ^@ http://purl.uniprot.org/uniprot/Q5SJH3 ^@ Function|||Similarity|||Subunit ^@ Belongs to the bacterial ribosomal protein bS16 family.|||Binds to the lower part of the body of the 30S subunit, where it stabilizes two of its domains.|||Part of the 30S ribosomal subunit. http://togogenome.org/gene/300852:TTH_RS00590 ^@ http://purl.uniprot.org/uniprot/Q5SM31 ^@ Cofactor|||Function|||Similarity ^@ Belongs to the Nth/MutY family.|||Binds 1 [4Fe-4S] cluster.|||DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate. http://togogenome.org/gene/300852:TTH_RS04310 ^@ http://purl.uniprot.org/uniprot/Q5SK18 ^@ Similarity ^@ Belongs to the AP endonuclease 2 family. http://togogenome.org/gene/300852:TTH_RS07320 ^@ http://purl.uniprot.org/uniprot/Q5SIC8 ^@ Similarity ^@ Belongs to the FBPase class 2 family. http://togogenome.org/gene/300852:TTH_RS09610 ^@ http://purl.uniprot.org/uniprot/Q56213 ^@ Function|||Subcellular Location Annotation|||Subunit ^@ Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source.|||Cytoplasm|||Homodimer. http://togogenome.org/gene/300852:TTH_RS05015 ^@ http://purl.uniprot.org/uniprot/Q5SJM1 ^@ Similarity ^@ Belongs to the thiolase-like superfamily. Thiolase family. http://togogenome.org/gene/300852:TTH_RS02820 ^@ http://purl.uniprot.org/uniprot/Q5SKV2 ^@ Domain|||Function|||Similarity ^@ Belongs to the RecA family. RadA subfamily.|||DNA-dependent ATPase involved in processing of recombination intermediates, plays a role in repairing DNA breaks. Stimulates the branch migration of RecA-mediated strand transfer reactions, allowing the 3' invading strand to extend heteroduplex DNA faster. Binds ssDNA in the presence of ADP but not other nucleotides, has ATPase activity that is stimulated by ssDNA and various branched DNA structures, but inhibited by SSB. Does not have RecA's homology-searching function.|||Plays a role in repairing double-strand DNA breaks, probably involving stabilizing or processing branched DNA or blocked replication forks.|||The middle region has homology to RecA with ATPase motifs including the RadA KNRFG motif, while the C-terminus is homologous to Lon protease. http://togogenome.org/gene/300852:TTH_RS07290 ^@ http://purl.uniprot.org/uniprot/Q56242 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the ABC transporter superfamily. UvrA family.|||Cytoplasm|||Forms a heterotetramer with UvrB during the search for lesions.|||The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrA is an ATPase and a DNA-binding protein. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. When the presence of a lesion has been verified by UvrB, the UvrA molecules dissociate. http://togogenome.org/gene/300852:TTH_RS05945 ^@ http://purl.uniprot.org/uniprot/Q5SJ42 ^@ Function|||Similarity ^@ Belongs to the glycogen phosphorylase family.|||Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. http://togogenome.org/gene/300852:TTH_RS00555 ^@ http://purl.uniprot.org/uniprot/Q5SM37 ^@ Cofactor|||Function|||Similarity ^@ Belongs to the ribulose-phosphate 3-epimerase family.|||Binds 1 divalent metal cation per subunit.|||Catalyzes the reversible epimerization of D-ribulose 5-phosphate to D-xylulose 5-phosphate. http://togogenome.org/gene/300852:TTH_RS08820 ^@ http://purl.uniprot.org/uniprot/Q5SHI8 ^@ Function|||Similarity|||Subunit ^@ Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.|||Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP).|||Homodimer. http://togogenome.org/gene/300852:TTH_RS03750 ^@ http://purl.uniprot.org/uniprot/Q5SKC1 ^@ Cofactor|||Function|||Similarity ^@ Belongs to the histidinol dehydrogenase family.|||Binds 1 zinc ion per subunit.|||Catalyzes the sequential NAD-dependent oxidations of L-histidinol to L-histidinaldehyde and then to L-histidine. http://togogenome.org/gene/300852:TTH_RS03180 ^@ http://purl.uniprot.org/uniprot/Q5SKN1 ^@ Cofactor|||Similarity|||Subunit ^@ Belongs to the CarB family.|||Binds 4 Mg(2+) or Mn(2+) ions per subunit.|||Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. http://togogenome.org/gene/300852:TTH_RS07930 ^@ http://purl.uniprot.org/uniprot/Q5SI14 ^@ Similarity ^@ Belongs to the BshC family. http://togogenome.org/gene/300852:TTH_RS06765 ^@ http://purl.uniprot.org/uniprot/Q5SIN2 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the binding-protein-dependent transport system permease family.|||Cell membrane|||Membrane http://togogenome.org/gene/300852:TTH_RS09720 ^@ http://purl.uniprot.org/uniprot/Q5SH13 ^@ Caution|||Function|||Similarity ^@ Belongs to the UbiX/PAD1 family.|||Flavin prenyltransferase that catalyzes the synthesis of the prenylated FMN cofactor (prenyl-FMN) for 4-hydroxy-3-polyprenylbenzoic acid decarboxylase UbiD. The prenyltransferase is metal-independent and links a dimethylallyl moiety from dimethylallyl monophosphate (DMAP) to the flavin N5 and C6 atoms of FMN.|||Lacks conserved residue(s) required for the propagation of feature annotation. http://togogenome.org/gene/300852:TTH_RS08325 ^@ http://purl.uniprot.org/uniprot/Q5SHT5 ^@ Activity Regulation|||Disruption Phenotype|||Domain|||Function|||Similarity|||Subunit ^@ Belongs to the DNA mismatch repair MutS family. MutS2 subfamily.|||Contains an N-terminal DNA-binding domain, followed by a dimerization domain and a C-terminal domain, called the small MutS-related (Smr) domain, which is absent from MutS and contains the endonuclease activity. The Smr domain can also bind DNA.|||Disruption causes an increase in the frequency of recombination between perfectly matched sequences.|||Endonuclease that is involved in the suppression of homologous recombination and may therefore have a key role in the control of bacterial genetic diversity. Cleaves the phosphate backbone of oligodeoxynucleotides non-sequence-specifically at the 3' side of the phosphates. Preferably incises the branched DNA structures, especially the D-loop structure over the Holliday junction. Has ATPase activity. Binds to dsDNA but not to ssDNA.|||Homodimer. Interacts with MutL.|||Nuclease activity is stimulated by interaction with MutL. ATPase activity is stimulated by dsDNA. http://togogenome.org/gene/300852:TTH_RS09650 ^@ http://purl.uniprot.org/uniprot/Q5SH27 ^@ Caution|||Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the acetylglutamate kinase family. LysZ subfamily.|||Catalyzes the phosphorylation of LysW-gamma-alpha-aminoadipate.|||Cytoplasm|||Lacks conserved residue(s) required for the propagation of feature annotation. http://togogenome.org/gene/300852:TTH_RS04615 ^@ http://purl.uniprot.org/uniprot/Q5SJV7 ^@ Similarity ^@ Belongs to the universal stress protein A family. http://togogenome.org/gene/300852:TTH_RS10950 ^@ http://purl.uniprot.org/uniprot/Q53VY1 ^@ Function|||Similarity|||Subunit ^@ A component of Cascade, which participates in CRISPR interference, the third stage of CRISPR immunity. Cascade binds both crRNA and in a sequence-specific manner negatively supercoiled dsDNA target. This leads to the formation of an R-loop in which the crRNA binds the target DNA, displacing the noncomplementary strand. Cas3 is recruited to Cascade, probably via interactions with CasA, nicks target DNA and then unwinds and cleaves the target, leading to DNA degradation and invader neutralization. CasA is not required for formation of Cascade, but probably enhances binding to and subsequent recognition of both target dsDNA and ssDNA (By similarity).|||Belongs to the CRISPR associated protein CasA/Cse1 family. Type I-E/Ecoli subfamily.|||CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (By similarity).|||Monomer. Part of the Cascade ribonucleoprotein complex. Interacts directly with the 5' end of crRNA, CasB, CasD and CasE. Binding of target ssRNA or dsDNA causes a conformational change in the Cascade complex; CasA is required for high affinity target DNA binding. Interacts with Cas3 once Cascade has recognized target DNA (By similarity). http://togogenome.org/gene/300852:TTH_RS10835 ^@ http://purl.uniprot.org/uniprot/Q53W05 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the CRISPR system Cmr5 family.|||CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Part of the Cmr ribonucleoprotein complex (By similarity).|||Cytoplasm|||Homotrimer. http://togogenome.org/gene/300852:TTH_RS07715 ^@ http://purl.uniprot.org/uniprot/Q5SI56 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the SHMT family.|||Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism.|||Cytoplasm|||Homodimer. http://togogenome.org/gene/300852:TTH_RS05545 ^@ http://purl.uniprot.org/uniprot/Q5SJC5 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the FtsZ family.|||Cytoplasm|||Essential cell division protein that forms a contractile ring structure (Z ring) at the future cell division site. The regulation of the ring assembly controls the timing and the location of cell division. One of the functions of the FtsZ ring is to recruit other cell division proteins to the septum to produce a new cell wall between the dividing cells. Binds GTP and shows GTPase activity.|||Homodimer. Polymerizes to form a dynamic ring structure in a strictly GTP-dependent manner. Interacts directly with several other division proteins. http://togogenome.org/gene/300852:TTH_RS00580 ^@ http://purl.uniprot.org/uniprot/Q5SM33 ^@ Function|||Similarity ^@ Belongs to the MoeA family.|||Catalyzes the insertion of molybdate into adenylated molybdopterin with the concomitant release of AMP. http://togogenome.org/gene/300852:TTH_RS09230 ^@ http://purl.uniprot.org/uniprot/Q5SHB2 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the RecA family.|||Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with LexA causing its activation and leading to its autocatalytic cleavage.|||Cytoplasm http://togogenome.org/gene/300852:TTH_RS09850 ^@ http://purl.uniprot.org/uniprot/Q5SGZ1 ^@ Similarity ^@ Belongs to the Nudix hydrolase family. http://togogenome.org/gene/300852:TTH_RS03665 ^@ http://purl.uniprot.org/uniprot/Q5SKD9 ^@ Function|||Similarity|||Subunit ^@ Belongs to the PdxS/SNZ family.|||Catalyzes the formation of pyridoxal 5'-phosphate from ribose 5-phosphate (RBP), glyceraldehyde 3-phosphate (G3P) and ammonia. The ammonia is provided by the PdxT subunit. Can also use ribulose 5-phosphate and dihydroxyacetone phosphate as substrates, resulting from enzyme-catalyzed isomerization of RBP and G3P, respectively.|||In the presence of PdxT, forms a dodecamer of heterodimers. http://togogenome.org/gene/300852:TTH_RS05180 ^@ http://purl.uniprot.org/uniprot/Q5SJJ0 ^@ Function|||Similarity ^@ Belongs to the transposase mutator family.|||Required for the transposition of the insertion element. http://togogenome.org/gene/300852:TTH_RS01435 ^@ http://purl.uniprot.org/uniprot/Q5SLM2 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the chaperonin (HSP60) family.|||Cytoplasm|||Forms a cylinder of 14 subunits composed of two heptameric rings stacked back-to-back. Interacts with the co-chaperonin GroES.|||Together with its co-chaperonin GroES, plays an essential role in assisting protein folding. The GroEL-GroES system forms a nano-cage that allows encapsulation of the non-native substrate proteins and provides a physical environment optimized to promote and accelerate protein folding. http://togogenome.org/gene/300852:TTH_RS04160 ^@ http://purl.uniprot.org/uniprot/Q5SK48 ^@ Cofactor|||Function|||Similarity ^@ Belongs to the radical SAM superfamily. MqnE family.|||Binds 1 [4Fe-4S] cluster. The cluster is likely coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.|||Radical SAM enzyme that catalyzes the addition of the adenosyl radical to the double bond of 3-[(1-carboxyvinyl)oxy]benzoate, leading to aminodeoxyfutalosine (AFL), a key intermediate in the formation of menaquinone (MK, vitamin K2) from chorismate. http://togogenome.org/gene/300852:TTH_RS00125 ^@ http://purl.uniprot.org/uniprot/Q5SMC1 ^@ Function|||Similarity|||Subunit ^@ Belongs to the bacterial/plant glucose-1-phosphate adenylyltransferase family.|||Homotetramer.|||Involved in the biosynthesis of ADP-glucose, a building block required for the elongation reactions to produce glycogen. Catalyzes the reaction between ATP and alpha-D-glucose 1-phosphate (G1P) to produce pyrophosphate and ADP-Glc. http://togogenome.org/gene/300852:TTH_RS06790 ^@ http://purl.uniprot.org/uniprot/Q5SIM7 ^@ Subcellular Location Annotation ^@ Membrane http://togogenome.org/gene/300852:TTH_RS08200 ^@ http://purl.uniprot.org/uniprot/Q5SHW0 ^@ Activity Regulation|||Function|||Similarity|||Subunit ^@ Activity increases by approximately two-fold in the presence of GCBP.|||Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. MalY/PatB cystathionine beta-lyase subfamily.|||Catalyzes the transformation of cystathionine to homocysteine. Can also use cystine or S-methylcysteine as substrate.|||Interacts with GCBP (TTHA1554). http://togogenome.org/gene/300852:TTH_RS10050 ^@ http://purl.uniprot.org/uniprot/Q53WE5 ^@ Function|||Similarity ^@ Belongs to the PINc/VapC protein family.|||Toxic component of a toxin-antitoxin (TA) system. An RNase. http://togogenome.org/gene/300852:TTH_RS10990 ^@ http://purl.uniprot.org/uniprot/Q53WG5 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the FdhD family.|||Cytoplasm|||Required for formate dehydrogenase (FDH) activity. Acts as a sulfur carrier protein that transfers sulfur from IscS to the molybdenum cofactor prior to its insertion into FDH. http://togogenome.org/gene/300852:TTH_RS06980 ^@ http://purl.uniprot.org/uniprot/Q5SIJ1 ^@ Activity Regulation|||Cofactor|||Function|||Similarity|||Subunit ^@ Belongs to the isocitrate and isopropylmalate dehydrogenases family.|||Binds 1 Mg(2+) ion per subunit.|||Catalyzes the NAD(+)-dependent oxidative decarboxylation of homoisocitrate to 2-oxoadipate (alpha-ketoadipate), a reaction involved in lysine biosynthesis through the alpha-aminoadipate pathway (PubMed:21813504). In addition, has high activity with isocitrate, but is inactive with 3-isopropylmalate (By similarity).|||Competitively inhibited by (2S,3S)-thiahomoisocitrate in vitro.|||Homotetramer. Dimer of dimers. The homotetramer can transiently dissociate into homodimers. http://togogenome.org/gene/300852:TTH_RS04540 ^@ http://purl.uniprot.org/uniprot/Q5SJX2 ^@ Subcellular Location Annotation ^@ Membrane http://togogenome.org/gene/300852:TTH_RS02750 ^@ http://purl.uniprot.org/uniprot/Q5SKW5 ^@ Similarity ^@ Belongs to the Nudix hydrolase family. http://togogenome.org/gene/300852:TTH_RS06150 ^@ http://purl.uniprot.org/uniprot/Q5SJ03 ^@ Cofactor|||Function|||Similarity ^@ Belongs to the ketol-acid reductoisomerase family.|||Binds 2 magnesium ions per subunit.|||Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate. http://togogenome.org/gene/300852:TTH_RS02025 ^@ http://purl.uniprot.org/uniprot/Q5SLA7 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the TatA/E family.|||Cell inner membrane|||Forms a complex with TatC.|||Part of the twin-arginine translocation (Tat) system that transports large folded proteins containing a characteristic twin-arginine motif in their signal peptide across membranes. TatA could form the protein-conducting channel of the Tat system. http://togogenome.org/gene/300852:TTH_RS03980 ^@ http://purl.uniprot.org/uniprot/Q5SK81 ^@ Subcellular Location Annotation ^@ Membrane http://togogenome.org/gene/300852:TTH_RS01340 ^@ http://purl.uniprot.org/uniprot/Q5SLP1 ^@ Activity Regulation|||Cofactor|||Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family.|||Binds 2 Zn(2+) ions.|||Cytoplasm|||Has endoribonuclease activity towards 23S and 16S rRNA (in vitro).|||Inhibited by cadmium, cobalt, manganese, magnesium, calcium and nickel ions.|||Monomer. http://togogenome.org/gene/300852:TTH_RS02320 ^@ http://purl.uniprot.org/uniprot/P80340 ^@ Function|||Miscellaneous|||Similarity|||Subunit ^@ Belongs to the bacterial ribosomal protein bL34 family.|||Found on the solvent side of the large subunit.|||Part of the 50S ribosomal subunit.|||The open reading frame (ORF) for this protein is entirely within the ORF for the RNase P protein (RnaP). The two start codons are separated by four nucleotides. http://togogenome.org/gene/300852:TTH_RS03820 ^@ http://purl.uniprot.org/uniprot/Q5SKB3 ^@ Function|||Similarity ^@ Belongs to the cytidine and deoxycytidylate deaminase family.|||This enzyme scavenges exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis. http://togogenome.org/gene/300852:TTH_RS00495 ^@ http://purl.uniprot.org/uniprot/Q56227 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the complex I subunit 5 family.|||Cell inner membrane|||NDH-1 is composed of 15 different subunits, Nqo1 to Nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits Nqo7, Nqo8 and Nqo10 to Nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits Nqo1 to Nqo6, Nqo9 and Nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers.|||NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP. http://togogenome.org/gene/300852:TTH_RS10260 ^@ http://purl.uniprot.org/uniprot/Q53WA8 ^@ Similarity ^@ Belongs to the precorrin methyltransferase family. http://togogenome.org/gene/300852:TTH_RS06200 ^@ http://purl.uniprot.org/uniprot/Q5SIZ2 ^@ Subcellular Location Annotation ^@ Cell outer membrane|||Periplasm http://togogenome.org/gene/300852:TTH_RS00400 ^@ http://purl.uniprot.org/uniprot/Q5SM66 ^@ Similarity ^@ Belongs to the inositol monophosphatase superfamily. http://togogenome.org/gene/300852:TTH_RS07870 ^@ http://purl.uniprot.org/uniprot/Q5SI26 ^@ Function|||Subunit ^@ Binds to glutamine synthetase and cystathionine beta-lyase. May be utilized for the efficient use of nitrogen in the global nitrogen regulation of T.thermophilus.|||Interacts with glutamine synthetase (TTHA1329) and cystathionine beta-lyase (TTHA1620), but proteins do not form a ternary complex. http://togogenome.org/gene/300852:TTH_RS02170 ^@ http://purl.uniprot.org/uniprot/Q5SL77 ^@ Similarity ^@ Belongs to the fabD family. http://togogenome.org/gene/300852:TTH_RS05455 ^@ http://purl.uniprot.org/uniprot/Q5SJE2 ^@ Function|||Similarity ^@ Belongs to the eIF-2B alpha/beta/delta subunits family. MtnA subfamily.|||Catalyzes the interconversion of methylthioribose-1-phosphate (MTR-1-P) into methylthioribulose-1-phosphate (MTRu-1-P). http://togogenome.org/gene/300852:TTH_RS03410 ^@ http://purl.uniprot.org/uniprot/Q5SKI9 ^@ Function|||Similarity|||Subunit ^@ Belongs to the Mrp/NBP35 ATP-binding proteins family.|||Binds and transfers iron-sulfur (Fe-S) clusters to target apoproteins. Can hydrolyze ATP.|||Homodimer.|||In the C-terminal section; belongs to the Mrp/NBP35 ATP-binding proteins family.|||In the N-terminal section; belongs to the MIP18 family. http://togogenome.org/gene/300852:TTH_RS01600 ^@ http://purl.uniprot.org/uniprot/Q5SLI9 ^@ Similarity ^@ Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily. http://togogenome.org/gene/300852:TTH_RS05000 ^@ http://purl.uniprot.org/uniprot/Q5SJM4 ^@ Cofactor|||Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the quinolinate synthase family. Type 2 subfamily.|||Binds 1 [4Fe-4S] cluster per subunit.|||Catalyzes the condensation of iminoaspartate with dihydroxyacetone phosphate to form quinolinate.|||Cytoplasm http://togogenome.org/gene/300852:TTH_RS06300 ^@ http://purl.uniprot.org/uniprot/Q5SIX3 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the SEDS family. MrdB/RodA subfamily.|||Cell inner membrane|||Membrane|||Peptidoglycan polymerase that is essential for cell wall elongation. http://togogenome.org/gene/300852:TTH_RS07545 ^@ http://purl.uniprot.org/uniprot/Q56236 ^@ Cofactor|||Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the GrpE family.|||Cytoplasm|||Homodimer.|||Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with DnaK and GrpE. It is the nucleotide exchange factor for DnaK and may function as a thermosensor. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding.|||Required to stimulate the ATPase activity of DnaK. http://togogenome.org/gene/300852:TTH_RS10710 ^@ http://purl.uniprot.org/uniprot/Q53W26 ^@ Subcellular Location Annotation ^@ Cell membrane|||Membrane http://togogenome.org/gene/300852:TTH_RS07680 ^@ http://purl.uniprot.org/uniprot/Q5SI57 ^@ Function|||Subcellular Location Annotation|||Subunit ^@ Cytoplasm|||Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.|||Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL. http://togogenome.org/gene/300852:TTH_RS10540 ^@ http://purl.uniprot.org/uniprot/Q53W52 ^@ Caution|||Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the IPP isomerase type 2 family.|||Cytoplasm|||Homooctamer. Dimer of tetramers.|||Involved in the biosynthesis of isoprenoids. Catalyzes the 1,3-allylic rearrangement of the homoallylic substrate isopentenyl (IPP) to its allylic isomer, dimethylallyl diphosphate (DMAPP).|||Lacks conserved residue(s) required for the propagation of feature annotation. http://togogenome.org/gene/300852:TTH_RS03350 ^@ http://purl.uniprot.org/uniprot/Q5SKK2 ^@ Subcellular Location Annotation ^@ Membrane http://togogenome.org/gene/300852:TTH_RS05735 ^@ http://purl.uniprot.org/uniprot/Q5SJ85 ^@ Cofactor|||Disruption Phenotype|||Function|||Miscellaneous|||Similarity|||Subcellular Location Annotation ^@ Belongs to the arginase family.|||Binds 2 manganese ions per subunit.|||Cells lacking this gene show a significant defect of growth at 78 degrees Celsius and accumulate N1-aminopropylagmatine. The double mutant of speB and speE shows defective growth at 70 degrees Celsius and significant defective growth at 78 degrees Celsius. It accumulates agmatine and N1-aminopropylagmatine.|||Cytoplasm|||In T.thermophilus, the biosynthetic pathways of spermidine operates via N1-aminopropylagmatine without the production of putrescine.|||Involved in the biosynthesis of polyamines which are thought to support the growth of thermophilic microorganisms under high-temperature conditions. It seems that long-chain and branched-chain of polyamines effectively stabilize DNA and RNA, respectively. Catalyzes the decarboxylation of N1-(3-aminopropyl)agmatine to yield spermidine and urea. It cannot use agmatine as substrate. http://togogenome.org/gene/300852:TTH_RS01960 ^@ http://purl.uniprot.org/uniprot/Q5SLC1 ^@ Similarity ^@ Belongs to the UPF0102 family. http://togogenome.org/gene/300852:TTH_RS01610 ^@ http://purl.uniprot.org/uniprot/Q5SLI7 ^@ Cofactor|||Function|||Similarity|||Subunit ^@ Belongs to the queuine tRNA-ribosyltransferase family.|||Binds 1 zinc ion per subunit.|||Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form the product. After dissociation, two additional enzymatic reactions on the tRNA convert PreQ1 to queuine (Q), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).|||Homodimer. Within each dimer, one monomer is responsible for RNA recognition and catalysis, while the other monomer binds to the replacement base PreQ1. http://togogenome.org/gene/300852:TTH_RS09110 ^@ http://purl.uniprot.org/uniprot/Q5SHD6 ^@ Function|||Similarity ^@ Belongs to the methylglyoxal synthase family.|||Catalyzes the formation of methylglyoxal from dihydroxyacetone phosphate. http://togogenome.org/gene/300852:TTH_RS01895 ^@ http://purl.uniprot.org/uniprot/Q5SLD1 ^@ Similarity ^@ Belongs to the purine nucleoside phosphorylase YfiH/LACC1 family. http://togogenome.org/gene/300852:TTH_RS07905 ^@ http://purl.uniprot.org/uniprot/Q8RQE7 ^@ Function|||Similarity|||Subunit ^@ Belongs to the RNA polymerase subunit omega family.|||Promotes RNA polymerase assembly. Latches the N- and C-terminal regions of the beta' subunit thereby facilitating its interaction with the beta and alpha subunits.|||The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription. http://togogenome.org/gene/300852:TTH_RS07085 ^@ http://purl.uniprot.org/uniprot/Q5SIH0 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 2 subfamily.|||Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).|||Cytoplasm|||Homodimer. http://togogenome.org/gene/300852:TTH_RS01825 ^@ http://purl.uniprot.org/uniprot/Q5SLE6 ^@ Similarity ^@ Belongs to the cysteine synthase/cystathionine beta-synthase family. http://togogenome.org/gene/300852:TTH_RS05420 ^@ http://purl.uniprot.org/uniprot/Q5SJE9 ^@ Caution|||Function|||Similarity|||Subunit ^@ Belongs to the Rho family.|||Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template.|||Homohexamer. The homohexamer assembles into an open ring structure.|||Lacks conserved residue(s) required for the propagation of feature annotation. http://togogenome.org/gene/300852:TTH_RS07280 ^@ http://purl.uniprot.org/uniprot/Q5SID6 ^@ Similarity ^@ Belongs to the ComB family. http://togogenome.org/gene/300852:TTH_RS01380 ^@ http://purl.uniprot.org/uniprot/Q5SLN3 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the binding-protein-dependent transport system permease family. CysTW subfamily.|||Cell membrane|||Membrane|||Part of the binding-protein-dependent transport system for phosphate; probably responsible for the translocation of the substrate across the membrane. http://togogenome.org/gene/300852:TTH_RS05355 ^@ http://purl.uniprot.org/uniprot/Q5SJF6 ^@ Function|||Similarity ^@ Belongs to the PINc/VapC protein family.|||Toxic component of a toxin-antitoxin (TA) system. An RNase. http://togogenome.org/gene/300852:TTH_RS09215 ^@ http://purl.uniprot.org/uniprot/Q5SHB5 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the thioester dehydratase family. FabZ subfamily.|||Cytoplasm|||Involved in unsaturated fatty acids biosynthesis. Catalyzes the dehydration of short chain beta-hydroxyacyl-ACPs and long chain saturated and unsaturated beta-hydroxyacyl-ACPs. http://togogenome.org/gene/300852:TTH_RS08775 ^@ http://purl.uniprot.org/uniprot/Q5SHJ7 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.|||Cell membrane http://togogenome.org/gene/300852:TTH_RS08480 ^@ http://purl.uniprot.org/uniprot/Q5SHQ4 ^@ Function|||Similarity|||Subunit ^@ Belongs to the universal ribosomal protein uL18 family.|||Part of the 50S ribosomal subunit; part of the 5S rRNA/uL5/uL18/bL25 subcomplex. Contacts the 5S and probably the 23S rRNA; has been isolated in a complex with the 5S rRNA and uL25 (Ref.2).|||This is one of the proteins that binds and mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance. http://togogenome.org/gene/300852:TTH_RS02250 ^@ http://purl.uniprot.org/uniprot/Q5SL61 ^@ Activity Regulation|||Caution|||Function|||Similarity|||Subunit ^@ Belongs to the IMPDH/GMPR family.|||Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth.|||Homotetramer.|||Lacks conserved residue(s) required for the propagation of feature annotation.|||Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH. http://togogenome.org/gene/300852:TTH_RS08785 ^@ http://purl.uniprot.org/uniprot/Q5SHJ5 ^@ Similarity ^@ Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. Csd subfamily. http://togogenome.org/gene/300852:TTH_RS02615 ^@ http://purl.uniprot.org/uniprot/Q5SKZ2 ^@ Function|||Similarity ^@ Belongs to the PINc/VapC protein family.|||Toxic component of a toxin-antitoxin (TA) system. An RNase. http://togogenome.org/gene/300852:TTH_RS10535 ^@ http://purl.uniprot.org/uniprot/Q53W53 ^@ Similarity ^@ Belongs to the carotenoid/retinoid oxidoreductase family. http://togogenome.org/gene/300852:TTH_RS08255 ^@ http://purl.uniprot.org/uniprot/Q5SHU9 ^@ Function|||Similarity|||Subunit ^@ Belongs to the tRNA pseudouridine synthase TruA family.|||Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs.|||Homodimer. http://togogenome.org/gene/300852:TTH_RS01695 ^@ http://purl.uniprot.org/uniprot/Q5SLH2 ^@ Cofactor|||Function|||Similarity ^@ Belongs to the polypeptide deformylase family.|||Binds 1 Fe(2+) ion.|||Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions. http://togogenome.org/gene/300852:TTH_RS04060 ^@ http://purl.uniprot.org/uniprot/Q5SK65 ^@ Function|||Similarity ^@ Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrR subfamily.|||Probably regulates transcriptional attenuation of the pyrimidine nucleotide (pyr) operon in response to exogenous pyrimidines. In contrast to pyr attenuation in Bacillus, PyrR from Thermus could act as a translational repressor: the binding of PyrR at its proposed recognition site in the transcript would prevent initiation of translation of the leader peptide, resulting in terminator formation and reduced expression of downstream genes (By similarity). Also displays uracil phosphoribosyltransferase activity (By similarity). http://togogenome.org/gene/300852:TTH_RS11260 ^@ http://purl.uniprot.org/uniprot/Q53VT5 ^@ Similarity ^@ Belongs to the extradiol ring-cleavage dioxygenase family. http://togogenome.org/gene/300852:TTH_RS05765 ^@ http://purl.uniprot.org/uniprot/Q5SJ79 ^@ Cofactor|||Similarity|||Subcellular Location Annotation ^@ Belongs to the heme-copper respiratory oxidase family.|||Binds 2 heme groups.|||Binds a copper B center.|||Cell membrane http://togogenome.org/gene/300852:TTH_RS01020 ^@ http://purl.uniprot.org/uniprot/Q5SLU8 ^@ Subcellular Location Annotation ^@ Cell inner membrane|||Membrane http://togogenome.org/gene/300852:TTH_RS04710 ^@ http://purl.uniprot.org/uniprot/Q56416 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the CoaE family.|||Catalyzes the phosphorylation of the 3'-hydroxyl group of dephosphocoenzyme A to form coenzyme A.|||Cytoplasm http://togogenome.org/gene/300852:TTH_RS11035 ^@ http://purl.uniprot.org/uniprot/Q53VX2 ^@ Similarity ^@ Belongs to the acyl coenzyme A hydrolase family. http://togogenome.org/gene/300852:TTH_RS07445 ^@ http://purl.uniprot.org/uniprot/Q5SIA4 ^@ Function|||Similarity ^@ Belongs to the helicase family. DnaB subfamily.|||Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity. http://togogenome.org/gene/300852:TTH_RS05985 ^@ http://purl.uniprot.org/uniprot/Q5SJ34 ^@ Similarity ^@ Belongs to the low molecular weight phosphotyrosine protein phosphatase family. http://togogenome.org/gene/300852:TTH_RS07325 ^@ http://purl.uniprot.org/uniprot/Q5SIC7 ^@ Similarity ^@ Belongs to the AlaDH/PNT family. http://togogenome.org/gene/300852:TTH_RS00485 ^@ http://purl.uniprot.org/uniprot/Q56225 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the complex I subunit 6 family.|||Cell inner membrane|||NDH-1 is composed of 15 different subunits, Nqo1 to Nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits Nqo7, Nqo8 and Nqo10 to Nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits Nqo1 to Nqo6, Nqo9 and Nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers.|||NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP. http://togogenome.org/gene/300852:TTH_RS08525 ^@ http://purl.uniprot.org/uniprot/P60489 ^@ Function|||Similarity|||Subunit ^@ Belongs to the universal ribosomal protein uL16 family.|||Part of the 50S ribosomal subunit.|||This protein binds directly to 23S rRNA. Interacts with the A site tRNA. http://togogenome.org/gene/300852:TTH_RS01355 ^@ http://purl.uniprot.org/uniprot/Q5SLN8 ^@ Cofactor|||Similarity ^@ Belongs to the Fur family.|||Binds 1 zinc ion per subunit. http://togogenome.org/gene/300852:TTH_RS03590 ^@ http://purl.uniprot.org/uniprot/Q5SKF3 ^@ Similarity ^@ Belongs to the thiolase-like superfamily. Thiolase family. http://togogenome.org/gene/300852:TTH_RS05265 ^@ http://purl.uniprot.org/uniprot/Q5SJH4 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ A probable RNA chaperone. Forms a complex with KhpB which binds to cellular RNA and controls its expression. Plays a role in peptidoglycan (PG) homeostasis and cell length regulation.|||Belongs to the KhpA RNA-binding protein family.|||Cytoplasm|||Forms a complex with KhpB. http://togogenome.org/gene/300852:TTH_RS05525 ^@ http://purl.uniprot.org/uniprot/Q5SJC9 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the MurCDEF family.|||Cell wall formation.|||Cytoplasm http://togogenome.org/gene/300852:TTH_RS02985 ^@ http://purl.uniprot.org/uniprot/Q9LCX3 ^@ Function|||Similarity|||Subunit ^@ Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln). Also acts on Asp-tRNA(Asn).|||Belongs to the amidase family. GatA subfamily.|||Heterotrimer of A, B and C subunits. http://togogenome.org/gene/300852:TTH_RS08010 ^@ http://purl.uniprot.org/uniprot/Q5SHZ8 ^@ Function|||Similarity|||Subunit ^@ Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.|||Catalyzes the decarboxylative condensation of pimeloyl-[acyl-carrier protein] and L-alanine to produce 8-amino-7-oxononanoate (AON), [acyl-carrier protein], and carbon dioxide (By similarity). Can also use pimeloyl-CoA instead of pimeloyl-ACP as substrate. It also converts 2-amino-3-ketobutyrate and CoA to glycine and acetyl-CoA. Activity is also observed with the following combinations of substrates: acetyl-CoA and either L-alanine or L-serine, pimeloyl-CoA and either glycine or L-serine, and palmitoyl-CoA with L-alanine.|||Homodimer. http://togogenome.org/gene/300852:TTH_RS05530 ^@ http://purl.uniprot.org/uniprot/Q5SJC8 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the MurB family.|||Cell wall formation.|||Cytoplasm http://togogenome.org/gene/300852:TTH_RS06160 ^@ http://purl.uniprot.org/uniprot/Q5SJ01 ^@ Cofactor|||Similarity ^@ Belongs to the TPP enzyme family.|||Binds 1 Mg(2+) ion per subunit.|||Binds 1 thiamine pyrophosphate per subunit. http://togogenome.org/gene/300852:TTH_RS06650 ^@ http://purl.uniprot.org/uniprot/Q5SIQ6 ^@ Subcellular Location Annotation ^@ Membrane http://togogenome.org/gene/300852:TTH_RS09635 ^@ http://purl.uniprot.org/uniprot/Q5SH30 ^@ Similarity ^@ Belongs to the NAD synthetase family. http://togogenome.org/gene/300852:TTH_RS08575 ^@ http://purl.uniprot.org/uniprot/Q5SHN5 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.|||Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.|||Cytoplasm http://togogenome.org/gene/300852:TTH_RS09975 ^@ http://purl.uniprot.org/uniprot/P38576 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the PPase family.|||Catalyzes the hydrolysis of inorganic pyrophosphate (PPi) forming two phosphate ions.|||Cytoplasm|||Homohexamer. http://togogenome.org/gene/300852:TTH_RS07050 ^@ http://purl.uniprot.org/uniprot/Q5SIH7 ^@ Cofactor|||Domain|||Function|||Similarity|||Subcellular Location Annotation ^@ 2 residues (Tyr-66 and Arg-69) present in a large hydrophobic pocket are probably involved in substrate specificity. They are important for desuccinylation activity, but dispensable for deacetylation activity.|||Belongs to the sirtuin family. Class III subfamily.|||Binds 1 zinc ion per subunit.|||Cytoplasm|||NAD-dependent lysine deacetylase and desuccinylase that specifically removes acetyl and succinyl groups on target proteins. Modulates the activities of several proteins which are inactive in their acylated form. http://togogenome.org/gene/300852:TTH_RS04040 ^@ http://purl.uniprot.org/uniprot/Q5SK69 ^@ Cofactor|||Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.|||Binds 1 FMN per subunit.|||Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor.|||Cell membrane|||Monomer. http://togogenome.org/gene/300852:TTH_RS10985 ^@ http://purl.uniprot.org/uniprot/Q53WG6 ^@ Function|||Similarity|||Subunit ^@ Belongs to the LamB/PxpA family.|||Catalyzes the cleavage of 5-oxoproline to form L-glutamate coupled to the hydrolysis of ATP to ADP and inorganic phosphate.|||Forms a complex composed of PxpA, PxpB and PxpC. http://togogenome.org/gene/300852:TTH_RS00295 ^@ http://purl.uniprot.org/uniprot/Q5SM87 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the ammonia transporter channel (TC 1.A.11.2) family.|||Cell membrane|||Membrane http://togogenome.org/gene/300852:TTH_RS01145 ^@ http://purl.uniprot.org/uniprot/Q5SLS3 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the purine/pyrimidine phosphoribosyltransferase family.|||Cytoplasm http://togogenome.org/gene/300852:TTH_RS01390 ^@ http://purl.uniprot.org/uniprot/Q5SLN1 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the ABC transporter superfamily. Phosphate importer (TC 3.A.1.7) family.|||Cell inner membrane|||Part of the ABC transporter complex PstSACB involved in phosphate import. Responsible for energy coupling to the transport system.|||The complex is composed of two ATP-binding proteins (PstB), two transmembrane proteins (PstC and PstA) and a solute-binding protein (PstS). http://togogenome.org/gene/300852:TTH_RS04430 ^@ http://purl.uniprot.org/uniprot/Q9WX76 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the RRF family.|||Cytoplasm|||Responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis. May increase the efficiency of translation by recycling ribosomes from one round of translation to another. http://togogenome.org/gene/300852:TTH_RS07900 ^@ http://purl.uniprot.org/uniprot/Q5SI20 ^@ Caution|||Cofactor|||Function|||Similarity ^@ Binds 1 FMN per subunit.|||Catalyzes two sequential steps in the biosynthesis of coenzyme A. In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine. In the second step the latter compound is decarboxylated to form 4'-phosphopantotheine.|||Catalyzes two steps in the biosynthesis of coenzyme A. In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine.|||In the C-terminal section; belongs to the PPC synthetase family.|||In the N-terminal section; belongs to the HFCD (homo-oligomeric flavin containing Cys decarboxylase) superfamily.|||Lacks conserved residue(s) required for the propagation of feature annotation. http://togogenome.org/gene/300852:TTH_RS02545 ^@ http://purl.uniprot.org/uniprot/Q5SL04 ^@ Similarity ^@ Belongs to the homoserine dehydrogenase family. http://togogenome.org/gene/300852:TTH_RS09410 ^@ http://purl.uniprot.org/uniprot/Q5SH75 ^@ Subcellular Location Annotation ^@ Membrane http://togogenome.org/gene/300852:TTH_RS09080 ^@ http://purl.uniprot.org/uniprot/Q5SHE1 ^@ Function|||Similarity|||Subunit ^@ Belongs to the MoaC family.|||Catalyzes the conversion of (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate to cyclic pyranopterin monophosphate (cPMP).|||Homohexamer; trimer of dimers. http://togogenome.org/gene/300852:TTH_RS00585 ^@ http://purl.uniprot.org/uniprot/Q5SM32 ^@ Subcellular Location Annotation ^@ Membrane http://togogenome.org/gene/300852:TTH_RS01050 ^@ http://purl.uniprot.org/uniprot/Q5SLU2 ^@ Similarity ^@ Belongs to the Mg-chelatase subunits D/I family. ComM subfamily. http://togogenome.org/gene/300852:TTH_RS02725 ^@ http://purl.uniprot.org/uniprot/Q5SKX0 ^@ Function|||Similarity|||Subunit ^@ Belongs to the GcvT family.|||The glycine cleavage system catalyzes the degradation of glycine.|||The glycine cleavage system is composed of four proteins: P, T, L and H. http://togogenome.org/gene/300852:TTH_RS03095 ^@ http://purl.uniprot.org/uniprot/Q5SKP7 ^@ Similarity ^@ Belongs to the bacterial solute-binding protein 9 family. http://togogenome.org/gene/300852:TTH_RS06735 ^@ http://purl.uniprot.org/uniprot/Q5SIN9 ^@ Similarity ^@ Belongs to the leucine-binding protein family. http://togogenome.org/gene/300852:TTH_RS10365 ^@ http://purl.uniprot.org/uniprot/Q53W87 ^@ Subcellular Location Annotation ^@ Membrane http://togogenome.org/gene/300852:TTH_RS00680 ^@ http://purl.uniprot.org/uniprot/Q5SM14 ^@ Function|||Miscellaneous|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the class-II aminoacyl-tRNA synthetase family. HisZ subfamily.|||Cytoplasm|||Heteromultimer composed of HisG and HisZ subunits.|||Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine.|||This function is generally fulfilled by the C-terminal part of HisG, which is missing in some bacteria such as this one. http://togogenome.org/gene/300852:TTH_RS10660 ^@ http://purl.uniprot.org/uniprot/Q53W34 ^@ Function|||Similarity ^@ Belongs to the MsrA Met sulfoxide reductase family.|||Has an important function as a repair enzyme for proteins that have been inactivated by oxidation. Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine. http://togogenome.org/gene/300852:TTH_RS03030 ^@ http://purl.uniprot.org/uniprot/P68591 ^@ Similarity ^@ Belongs to the UPF0340 family. http://togogenome.org/gene/300852:TTH_RS01265 ^@ http://purl.uniprot.org/uniprot/Q5SLQ1 ^@ Function|||Similarity|||Subunit ^@ Belongs to the bacterial ribosomal protein bL9 family.|||Binds to the 23S rRNA. Extends more that 50 Angstroms beyond the surface of the 70S ribosome.|||Part of the 50S ribosomal subunit. http://togogenome.org/gene/300852:TTH_RS01195 ^@ http://purl.uniprot.org/uniprot/Q5SLR3 ^@ Function|||Subunit ^@ Heterotetramer of two alpha and two beta chains. Directly associated with ODBA in the E1 complex.|||The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3) (By similarity). http://togogenome.org/gene/300852:TTH_RS07100 ^@ http://purl.uniprot.org/uniprot/Q5SIG7 ^@ Function|||Similarity ^@ Belongs to the CcmH/CycL/Ccl2/NrfF family.|||Possible subunit of a heme lyase. http://togogenome.org/gene/300852:TTH_RS06270 ^@ http://purl.uniprot.org/uniprot/Q5SIY0 ^@ Cofactor|||Function|||Similarity|||Subunit ^@ Belongs to the IlvD/Edd family.|||Binds 1 [2Fe-2S] cluster per subunit. This cluster acts as a Lewis acid cofactor.|||Functions in the biosynthesis of branched-chain amino acids. Catalyzes the dehydration of (2R,3R)-2,3-dihydroxy-3-methylpentanoate (2,3-dihydroxy-3-methylvalerate) into 2-oxo-3-methylpentanoate (2-oxo-3-methylvalerate) and of (2R)-2,3-dihydroxy-3-methylbutanoate (2,3-dihydroxyisovalerate) into 2-oxo-3-methylbutanoate (2-oxoisovalerate), the penultimate precursor to L-isoleucine and L-valine, respectively.|||Homodimer. http://togogenome.org/gene/300852:TTH_RS03165 ^@ http://purl.uniprot.org/uniprot/Q5SKN4 ^@ Domain|||Function|||Similarity|||Subunit ^@ Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM61 family.|||Catalyzes the S-adenosyl-L-methionine-dependent formation of N(1)-methyladenine at position 58 (m1A58) in tRNA.|||Contains a large catalytic C-terminal domain that binds S-adenosyl-L-methionine and a smaller N-terminal domain that may play a role in tRNA recognition. Domains are connected by a linker region.|||Homotetramer composed of a dimer of dimers. http://togogenome.org/gene/300852:TTH_RS07890 ^@ http://purl.uniprot.org/uniprot/Q5SI22 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the UPF0056 (MarC) family.|||Cell inner membrane|||Membrane http://togogenome.org/gene/300852:TTH_RS07660 ^@ http://purl.uniprot.org/uniprot/Q5SI61 ^@ Similarity ^@ Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily. http://togogenome.org/gene/300852:TTH_RS10110 ^@ http://purl.uniprot.org/uniprot/Q53WE0 ^@ Similarity ^@ Belongs to the acyl-CoA dehydrogenase family. http://togogenome.org/gene/300852:TTH_RS00670 ^@ http://purl.uniprot.org/uniprot/Q5SM16 ^@ Domain|||Function|||Miscellaneous|||Similarity|||Subunit ^@ Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family.|||Binding of TrmH to tRNA is composed of at least three steps: the first is bi-molecular binding and the subsequent two are uni-molecular induced-fit processes.|||Both N- and C-terminal regions function in tRNA binding, but the substrate tRNA is determined by the catalytic domain.|||Catalyzes the 2'-O methylation of guanosine at position 18 in tRNA. Type I methylase, which methylates all tRNAs.|||Homodimer. http://togogenome.org/gene/300852:TTH_RS03450 ^@ http://purl.uniprot.org/uniprot/Q5SKI2 ^@ Subcellular Location Annotation ^@ Membrane http://togogenome.org/gene/300852:TTH_RS10150 ^@ http://purl.uniprot.org/uniprot/Q53WD3 ^@ Function|||Similarity|||Subunit ^@ Belongs to the YdjC deacetylase family.|||Homodimer.|||Probably catalyzes the deacetylation of acetylated carbohydrates an important step in the degradation of oligosaccharides. http://togogenome.org/gene/300852:TTH_RS02810 ^@ http://purl.uniprot.org/uniprot/Q5SKV4 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the branched-chain polyamine synthase family.|||Cytoplasm|||Involved in the biosynthesis of branched-chain polyamines, which support the growth of thermophiles under high-temperature conditions. Catalyzes the sequential condensation of spermidine with the aminopropyl groups of decarboxylated S-adenosylmethionines to produce N(4)-bis(aminopropyl)spermidine via N(4)-aminopropylspermidine. http://togogenome.org/gene/300852:TTH_RS04050 ^@ http://purl.uniprot.org/uniprot/Q5SK67 ^@ Cofactor|||Function|||Similarity ^@ Belongs to the metallo-dependent hydrolases superfamily. DHOase family. Class I DHOase subfamily.|||Binds 2 Zn(2+) ions per subunit.|||Catalyzes the reversible cyclization of carbamoyl aspartate to dihydroorotate. http://togogenome.org/gene/300852:TTH_RS01885 ^@ http://purl.uniprot.org/uniprot/Q5SLD4 ^@ Subcellular Location Annotation ^@ Cytoplasm http://togogenome.org/gene/300852:TTH_RS10955 ^@ http://purl.uniprot.org/uniprot/Q53VY0 ^@ Function|||Similarity|||Subunit ^@ Belongs to the CRISPR-associated CasB/Cse2 family.|||CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (By similarity).|||Homodimer. http://togogenome.org/gene/300852:TTH_RS10220 ^@ http://purl.uniprot.org/uniprot/Q53WB6 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the CobS family.|||Cell inner membrane|||Joins adenosylcobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin). Also synthesizes adenosylcobalamin 5'-phosphate from adenosylcobinamide-GDP and alpha-ribazole 5'-phosphate.|||Membrane http://togogenome.org/gene/300852:TTH_RS09625 ^@ http://purl.uniprot.org/uniprot/Q5SH32 ^@ Similarity ^@ Belongs to the Nth/MutY family. http://togogenome.org/gene/300852:TTH_RS08415 ^@ http://purl.uniprot.org/uniprot/Q9Z9H5 ^@ Similarity|||Subunit ^@ Belongs to the bacterial ribosomal protein bL17 family.|||Part of the 50S ribosomal subunit. Contacts protein L32. http://togogenome.org/gene/300852:TTH_RS08995 ^@ http://purl.uniprot.org/uniprot/Q5SHF6 ^@ Similarity ^@ Belongs to the GSP E family. http://togogenome.org/gene/300852:TTH_RS06595 ^@ http://purl.uniprot.org/uniprot/Q5SIR2 ^@ Subcellular Location Annotation ^@ Membrane http://togogenome.org/gene/300852:TTH_RS06745 ^@ http://purl.uniprot.org/uniprot/Q5SIN7 ^@ Subcellular Location Annotation ^@ Membrane http://togogenome.org/gene/300852:TTH_RS01095 ^@ http://purl.uniprot.org/uniprot/Q8VVE2 ^@ Function|||PTM|||Similarity|||Subunit ^@ Belongs to the bacterial ribosomal protein bL12 family.|||Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. Is thus essential for accurate translation. It extends beyond the surface of the 70S ribosome. The stalk is preferentially stabilized in 70S versus 50S crystals.|||Homodimer. Part of the 50S ribosomal subunit; present in 6 copies per ribosome. Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. Forms a heptameric L10(L12)2(L12)2(L12)2 complex, where L10 forms an elongated spine to which 3 L12 dimers bind in a sequential fashion. Contacts protein L11.|||The ion at m/z 12978 is suggested to be the N-acetylated form of the protein (L7). The ion at m/z 12964 is suggested to be consistent with dimethylation or formylation and is present in very small quantities in the sample.|||The ion at m/z 13016 is probably phosphorylated; treatment with phosphatase in the presence of kinase inhibitors decreases it weight. This form is only detected on 70S ribosomes and not in isolated ribosomal stalk complexes. http://togogenome.org/gene/300852:TTH_RS03425 ^@ http://purl.uniprot.org/uniprot/Q5SKI6 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the RNA methyltransferase RsmE family.|||Cytoplasm|||Specifically methylates the N3 position of the uracil ring of uridine 1498 (m3U1498) in 16S rRNA. Acts on the fully assembled 30S ribosomal subunit. http://togogenome.org/gene/300852:TTH_RS06740 ^@ http://purl.uniprot.org/uniprot/Q5SIN8 ^@ Subcellular Location Annotation ^@ Membrane http://togogenome.org/gene/300852:TTH_RS05430 ^@ http://purl.uniprot.org/uniprot/P56690 ^@ Cofactor|||Domain|||Function|||Miscellaneous|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.|||Binds 2 Zn(2+) ions per subunit.|||Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) (By similarity).|||Cytoplasm|||IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) (By similarity).|||Monomer.|||The ATP consumption with regard to L-valine is nonproductive and is solely for substrate selection, which demonstrates the high cost of accuracy. http://togogenome.org/gene/300852:TTH_RS05580 ^@ http://purl.uniprot.org/uniprot/Q5SJB8 ^@ Cofactor|||Function|||Similarity|||Subunit ^@ Belongs to the thymidylate synthase ThyX family.|||Binds 4 FAD per tetramer. Each FAD binding site is formed by three monomers.|||Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor, and NADPH and FADH(2) as the reductant.|||Homotetramer. http://togogenome.org/gene/300852:TTH_RS05590 ^@ http://purl.uniprot.org/uniprot/Q5SJB6 ^@ Similarity ^@ Belongs to the BMP lipoprotein family. http://togogenome.org/gene/300852:TTH_RS03985 ^@ http://purl.uniprot.org/uniprot/Q5SK80 ^@ Subcellular Location Annotation ^@ Cell inner membrane|||Membrane http://togogenome.org/gene/300852:TTH_RS09645 ^@ http://purl.uniprot.org/uniprot/Q5SH28 ^@ Function|||Similarity ^@ Belongs to the glycosyl hydrolase 57 family.|||Catalyzes the formation of branch points in alpha-glucans by cleavage of an alpha-1,4 glycosidic bond and subsequent transfer of the cleaved-off oligosaccharide to a new alpha-1,6 position. The branch chain-length distribution of the reaction products shows degree of polymerization (DP) of 3 to 13, with two local maxima at DP 7 and DP 11. Exhibits an alpha-retaining catalytic mechanism. Is involved in glycogen biosynthesis. Shows a secondary activity, i.e. the hydrolysis of the substrate, being 4% of the total activity. Can use amylose as substrate but not alpha-1,4-linked oligosaccharides of 2-7 glucose residues, beta-cyclodextrin, 6-O-glucosyl-beta-cyclodextrin and 6-O-maltosyl-beta-cyclodextrin. Is not able to branch amylopectin further, it only hydrolyzes amylopectin. Thus, displays preference for linear and long substrates (amylose) over branched structures (amylopectin). http://togogenome.org/gene/300852:TTH_RS03460 ^@ http://purl.uniprot.org/uniprot/Q5SKI0 ^@ Similarity ^@ Belongs to the PEP-utilizing enzyme family. http://togogenome.org/gene/300852:TTH_RS03680 ^@ http://purl.uniprot.org/uniprot/Q5SKD6 ^@ Function|||Similarity|||Subunit ^@ Belongs to the glutaminase PdxT/SNO family.|||Catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the biosynthesis of pyridoxal 5'-phosphate. The resulting ammonia molecule is channeled to the active site of PdxS.|||In the presence of PdxS, forms a dodecamer of heterodimers. Only shows activity in the heterodimer. http://togogenome.org/gene/300852:TTH_RS04740 ^@ http://purl.uniprot.org/uniprot/Q5SJS7 ^@ Cofactor|||Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. TrmE GTPase family.|||Binds 1 potassium ion per subunit.|||Cytoplasm|||Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34.|||Homodimer. Heterotetramer of two MnmE and two MnmG subunits. http://togogenome.org/gene/300852:TTH_RS07670 ^@ http://purl.uniprot.org/uniprot/Q5SI59 ^@ Similarity ^@ Belongs to the SAICAR synthetase family. http://togogenome.org/gene/300852:TTH_RS02130 ^@ http://purl.uniprot.org/uniprot/Q5SL85 ^@ Function|||Similarity ^@ Belongs to the pseudouridine synthase RluA family.|||Responsible for synthesis of pseudouridine from uracil. http://togogenome.org/gene/300852:TTH_RS10705 ^@ http://purl.uniprot.org/uniprot/Q53W27 ^@ Subcellular Location Annotation ^@ Membrane http://togogenome.org/gene/300852:TTH_RS07675 ^@ http://purl.uniprot.org/uniprot/Q5SI58 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the PurS family.|||Cytoplasm|||Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.|||Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL. http://togogenome.org/gene/300852:TTH_RS05415 ^@ http://purl.uniprot.org/uniprot/Q5SJF0 ^@ Cofactor|||Function|||Similarity ^@ Binds 1 zinc ion.|||Converts 2,5-diamino-6-(ribosylamino)-4(3h)-pyrimidinone 5'-phosphate into 5-amino-6-(ribosylamino)-2,4(1h,3h)-pyrimidinedione 5'-phosphate.|||In the C-terminal section; belongs to the HTP reductase family.|||In the N-terminal section; belongs to the cytidine and deoxycytidylate deaminase family. http://togogenome.org/gene/300852:TTH_RS00075 ^@ http://purl.uniprot.org/uniprot/Q5SMD0 ^@ Similarity ^@ Belongs to the FPP/GGPP synthase family. http://togogenome.org/gene/300852:TTH_RS09915 ^@ http://purl.uniprot.org/uniprot/Q5SGX8 ^@ Function|||Similarity|||Subunit ^@ Belongs to the DnaX/STICHEL family.|||DNA polymerase III contains a core (composed of alpha, epsilon and theta chains) that associates with a tau subunit. This core dimerizes to form the POLIII' complex. PolIII' associates with the gamma complex (composed of gamma, delta, delta', psi and chi chains) and with the beta chain to form the complete DNA polymerase III complex.|||DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. http://togogenome.org/gene/300852:TTH_RS09795 ^@ http://purl.uniprot.org/uniprot/Q5SH02 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the gamma-glutamyl phosphate reductase family.|||Catalyzes the NADPH-dependent reduction of L-glutamate 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate.|||Cytoplasm http://togogenome.org/gene/300852:TTH_RS03635 ^@ http://purl.uniprot.org/uniprot/P48515 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily.|||Cytoplasm|||One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex (By similarity). http://togogenome.org/gene/300852:TTH_RS07980 ^@ http://purl.uniprot.org/uniprot/Q5SI04 ^@ Similarity ^@ Belongs to the Glu/Leu/Phe/Val dehydrogenases family. http://togogenome.org/gene/300852:TTH_RS05930 ^@ http://purl.uniprot.org/uniprot/Q5SJ45 ^@ Cofactor|||Domain|||Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 1 subfamily.|||Binds 2 Zn(2+) ions per subunit.|||Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a 'posttransfer' editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner.|||Cytoplasm|||Monomer.|||The C-terminal coiled-coil domain is crucial for aminoacylation activity.|||ValRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated threonine is translocated from the active site to the editing site. http://togogenome.org/gene/300852:TTH_RS07530 ^@ http://purl.uniprot.org/uniprot/Q9RA63 ^@ Domain|||Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the ClpA/ClpB family.|||Cytoplasm|||Homohexamer. The oligomerization is ATP-dependent.|||Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase activity; ATP hydrolysis unfolds the denatured protein aggregates, which probably helps expose new hydrophobic binding sites on the surface of ClpB-bound aggregates, contributing to the solubilization and refolding of denatured protein aggregates by DnaK.|||The Clp repeat (R) domain probably functions as a substrate-discriminating domain, recruiting aggregated proteins to the ClpB hexamer and/or stabilizing bound proteins. The NBD2 domain is responsible for oligomerization, whereas the NBD1 domain stabilizes the hexamer probably in an ATP-dependent manner. The movement of the coiled-coil domain is essential for ClpB ability to rescue proteins from an aggregated state, probably by pulling apart large aggregated proteins, which are bound between the coiled-coils motifs of adjacent ClpB subunits in the functional hexamer. http://togogenome.org/gene/300852:TTH_RS02105 ^@ http://purl.uniprot.org/uniprot/Q5SL90 ^@ Caution|||Cofactor|||Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins. Plays a role in the quality control of integral membrane proteins.|||Belongs to the AAA ATPase family.|||Binds 1 zinc ion per subunit.|||Cell inner membrane|||Homohexamer.|||In the C-terminal section; belongs to the peptidase M41 family.|||In the central section; belongs to the AAA ATPase family.|||Lacks conserved residue(s) required for the propagation of feature annotation. http://togogenome.org/gene/300852:TTH_RS08425 ^@ http://purl.uniprot.org/uniprot/P80373 ^@ Cofactor|||Function|||Similarity|||Subunit ^@ Belongs to the universal ribosomal protein uS4 family.|||Binds 1 zinc ion per subunit (PubMed:11866529). In a number of structures (e.g. 4Y4O, 5FDV) a 4Fe-4S cluster is seen in place of the zinc cofactor (PubMed:25775268, PubMed:26792896).|||One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it helps nucleate assembly of the body and platform of the 30S subunit. Binds mRNA in the 70S ribosome, positioning it for translation.|||Part of the 30S ribosomal subunit (PubMed:11014182, PubMed:11007480, PubMed:11163189, PubMed:11014183, PubMed:11511350, PubMed:11296217, PubMed:11228145, PubMed:11228145, PubMed:11283358, PubMed:11340196, PubMed:11866529, PubMed:25775268, PubMed:26792896). Contacts protein S5 (PubMed:11340196). The interaction surface between S4 and S5 is involved in control of translational fidelity. http://togogenome.org/gene/300852:TTH_RS05120 ^@ http://purl.uniprot.org/uniprot/Q5SJK0 ^@ Subcellular Location Annotation ^@ Membrane http://togogenome.org/gene/300852:TTH_RS04400 ^@ http://purl.uniprot.org/uniprot/Q5SK00 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the pyrroline-5-carboxylate reductase family.|||Catalyzes the reduction of 1-pyrroline-5-carboxylate (PCA) to L-proline.|||Cytoplasm http://togogenome.org/gene/300852:TTH_RS10005 ^@ http://purl.uniprot.org/uniprot/Q9LCY2 ^@ Disruption Phenotype|||Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the methyltransferase superfamily. RNA methyltransferase RsmG family.|||Cytoplasm|||Low-level streptomycin resistance.|||Specifically methylates the N7 position of guanine in position 527 of 16S rRNA. Shows a marked preference for deproteinized 16S rRNA as substrate and is completely inactive with native 30S subunits as substrate. http://togogenome.org/gene/300852:TTH_RS05570 ^@ http://purl.uniprot.org/uniprot/Q5SJC0 ^@ Function|||Similarity|||Subunit ^@ Belongs to the TrpA family.|||Tetramer of two alpha and two beta chains.|||The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate. http://togogenome.org/gene/300852:TTH_RS00335 ^@ http://purl.uniprot.org/uniprot/Q5SM79 ^@ Subcellular Location Annotation ^@ Membrane http://togogenome.org/gene/300852:TTH_RS09655 ^@ http://purl.uniprot.org/uniprot/Q5SH26 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the NAGSA dehydrogenase family. Type 1 subfamily. LysY sub-subfamily.|||Catalyzes the NADPH-dependent reduction of [LysW]-aminoadipate 6-phosphate to yield [LysW]-aminoadipate 6-semialdehyde.|||Cytoplasm http://togogenome.org/gene/300852:TTH_RS00685 ^@ http://purl.uniprot.org/uniprot/Q5SM13 ^@ Caution|||Function|||Similarity|||Subunit ^@ Belongs to the dCTP deaminase family.|||Catalyzes the deamination of dCTP to dUTP.|||Homotrimer.|||Lacks conserved residue(s) required for the propagation of feature annotation. http://togogenome.org/gene/300852:TTH_RS00605 ^@ http://purl.uniprot.org/uniprot/Q5SM28 ^@ Domain|||Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily.|||Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). Can inadvertently accommodate and process cysteine.|||Consists of three domains: the N-terminal catalytic domain, the anticodon-binding domain and the C-terminal extension. The C-terminal extension binds a zinc ion, which probably plays a non-essential structural role in stabilizing the fold of C-terminal domain.|||Cytoplasm|||Homodimer. Only one tRNA molecule binds per dimer. http://togogenome.org/gene/300852:TTH_RS11160 ^@ http://purl.uniprot.org/uniprot/Q5SJJ0 ^@ Function|||Similarity ^@ Belongs to the transposase mutator family.|||Required for the transposition of the insertion element. http://togogenome.org/gene/300852:TTH_RS08530 ^@ http://purl.uniprot.org/uniprot/P80372 ^@ Function|||Similarity|||Subunit ^@ Belongs to the universal ribosomal protein uS3 family.|||Binds the lower part of the 30S subunit head. Binds mRNA in the 70S ribosome, positioning it for translation.|||Part of the 30S ribosomal subunit. Forms a tight complex with proteins S10 and S14. http://togogenome.org/gene/300852:TTH_RS10245 ^@ http://purl.uniprot.org/uniprot/Q53WB1 ^@ Function|||Similarity ^@ Belongs to the CbiD family.|||Catalyzes the methylation of C-1 in cobalt-precorrin-5B to form cobalt-precorrin-6A. http://togogenome.org/gene/300852:TTH_RS05385 ^@ http://purl.uniprot.org/uniprot/Q5SMH0 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the QueA family.|||Cytoplasm|||Monomer.|||Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA). http://togogenome.org/gene/300852:TTH_RS06535 ^@ http://purl.uniprot.org/uniprot/Q5SIS5 ^@ Function|||Similarity|||Subunit ^@ Belongs to the SbcD family.|||Heterodimer of SbcC and SbcD.|||SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand endonuclease activity. http://togogenome.org/gene/300852:TTH_RS08460 ^@ http://purl.uniprot.org/uniprot/Q5SHQ8 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the SecY/SEC61-alpha family.|||Cell inner membrane|||Component of the Sec protein translocase complex. Heterotrimer consisting of SecY, SecE and SecG subunits. The heterotrimers can form oligomers, although 1 heterotrimer is thought to be able to translocate proteins. Interacts with the ribosome. Interacts with SecDF, and other proteins may be involved. Interacts with SecA.|||The central subunit of the protein translocation channel SecYEG. Consists of two halves formed by TMs 1-5 and 6-10. These two domains form a lateral gate at the front which open onto the bilayer between TMs 2 and 7, and are clamped together by SecE at the back. The channel is closed by both a pore ring composed of hydrophobic SecY resides and a short helix (helix 2A) on the extracellular side of the membrane which forms a plug. The plug probably moves laterally to allow the channel to open. The ring and the pore may move independently. http://togogenome.org/gene/300852:TTH_RS03105 ^@ http://purl.uniprot.org/uniprot/Q5SKP5 ^@ Subcellular Location Annotation ^@ Membrane http://togogenome.org/gene/300852:TTH_RS04980 ^@ http://purl.uniprot.org/uniprot/Q5SJM8 ^@ Caution|||Domain|||Function|||Similarity|||Subunit ^@ Belongs to the FBP aldolase/phosphatase family.|||Catalyzes two subsequent steps in gluconeogenesis: the aldol condensation of dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (GA3P) to fructose-1,6-bisphosphate (FBP), and the dephosphorylation of FBP to fructose-6-phosphate (F6P).|||Consists of a single catalytic domain, but remodels its active-site architecture via a large structural change to exhibit dual activities.|||Homooctamer; dimer of tetramers.|||Lacks conserved residue(s) required for the propagation of feature annotation. http://togogenome.org/gene/300852:TTH_RS01310 ^@ http://purl.uniprot.org/uniprot/P35871 ^@ Function|||Similarity|||Subunit ^@ Belongs to the bacterial ribosomal protein bL33 family.|||Contacts the E site tRNA.|||Found on the solvent side of the large subunit.|||Part of the 50S ribosomal subunit. http://togogenome.org/gene/300852:TTH_RS06795 ^@ http://purl.uniprot.org/uniprot/Q5SIM6 ^@ Similarity ^@ Belongs to the citrate synthase family. http://togogenome.org/gene/300852:TTH_RS00910 ^@ http://purl.uniprot.org/uniprot/Q5SLX0 ^@ Similarity ^@ Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. http://togogenome.org/gene/300852:TTH_RS10560 ^@ http://purl.uniprot.org/uniprot/Q53WF7 ^@ Cofactor|||Similarity ^@ Belongs to the galactose-1-phosphate uridylyltransferase type 1 family.|||Binds 1 zinc ion per subunit. http://togogenome.org/gene/300852:TTH_RS06920 ^@ http://purl.uniprot.org/uniprot/Q5SIK1 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the peptidase S26 family.|||Membrane http://togogenome.org/gene/300852:TTH_RS05970 ^@ http://purl.uniprot.org/uniprot/Q5SJ37 ^@ Cofactor|||Function|||Similarity|||Subunit ^@ Belongs to the pseudouridine-5'-phosphate glycosidase family.|||Binds 1 Mn(2+) ion per subunit.|||Catalyzes the reversible cleavage of pseudouridine 5'-phosphate (PsiMP) to ribose 5-phosphate and uracil. Functions biologically in the cleavage direction, as part of a pseudouridine degradation pathway.|||Homotrimer. http://togogenome.org/gene/300852:TTH_RS05395 ^@ http://purl.uniprot.org/uniprot/Q5SMG8 ^@ Activity Regulation|||Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the SLC41A transporter family.|||Cell inner membrane|||Highly selective magnesium channel that plays an important role in Mg(2+) homeostasis (PubMed:17700703, PubMed:19798051, PubMed:25367295, PubMed:33905418). Functions as a Mg(2+)-dependent gating channel (PubMed:19798051, PubMed:25367295, PubMed:33905418). Exhibits low activity with cobalt, suggesting that it might also be involved in the uptake of Co(2+) as a micronutrient (PubMed:19798051). Also exhibits low activity with Ca(2+), but it shows almost no activity with Mn(2+) (PubMed:25367295).|||Homodimer.|||The channel activity is regulated via the N-terminal cytoplasmic region, which acts as a Mg(2+) sensor to regulate the gating of the ion-conducting pore in response to the intracellular magnesium concentration (PubMed:19798051, PubMed:25367295, PubMed:33905418). Under high-intracellular magnesium conditions, binding of magnesium to the N-terminal cytoplasmic domain stabilizes the closed conformation of the channel (PubMed:19798051, PubMed:25367295, PubMed:33905418). Under low-intracellular magnesium conditions, the channel is in equilibrium between the open and closed states (PubMed:19798051). A cation-binding site within the membrane (M1) strictly recognizes the size and geometry of the Mg(2+) hydration shells, which may be important for the selective transport of Mg(2+) over other cations (PubMed:25367295). Cation-binding sites on the periplasmic side (M2 and M3) regulate channel opening and prevent conduction of near-cognate cations (PubMed:25367295). Binding of Mn(2+) to the periplasmic sites strongly inhibits the Mg(2+) transport activity (PubMed:25367295). In addition, activity is regulated by ATP, which binds to MgtE and modulates its Mg(2+)-dependent channel gating (PubMed:28747715). ATP binding enhances the intracellular domain affinity for Mg(2+) within physiological concentrations of this divalent cation, enabling MgtE to function as an in vivo Mg(2+) sensor (PubMed:28747715). ATP dissociation from MgtE upregulates Mg(2+) influx at both high and low intracellular Mg(2+) concentrations (PubMed:28747715). http://togogenome.org/gene/300852:TTH_RS09715 ^@ http://purl.uniprot.org/uniprot/Q5SH14 ^@ Similarity ^@ Belongs to the acyl-CoA dehydrogenase family. http://togogenome.org/gene/300852:TTH_RS08615 ^@ http://purl.uniprot.org/uniprot/Q5SHM7 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the alanine or glycine:cation symporter (AGCS) (TC 2.A.25) family.|||Cell membrane|||Membrane http://togogenome.org/gene/300852:TTH_RS03040 ^@ http://purl.uniprot.org/uniprot/Q5SKQ8 ^@ Subcellular Location Annotation ^@ Membrane http://togogenome.org/gene/300852:TTH_RS08560 ^@ http://purl.uniprot.org/uniprot/Q5SHN8 ^@ Function|||PTM|||Similarity|||Subunit ^@ Belongs to the universal ribosomal protein uL3 family.|||One of the primary rRNA binding proteins, it binds directly near the 3'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit.|||Part of the 50S ribosomal subunit. Forms a cluster with proteins L14 and L19.|||The mass differences between the observed and calculated masses are suggested to be due to methylation, which is known to occur in the E.coli and R.palustris orthologs. http://togogenome.org/gene/300852:TTH_RS01170 ^@ http://purl.uniprot.org/uniprot/Q5SLR8 ^@ Caution|||Function|||Similarity ^@ Acts both as a biotin--[acetyl-CoA-carboxylase] ligase and a repressor.|||Belongs to the biotin--protein ligase family.|||Lacks conserved residue(s) required for the propagation of feature annotation. http://togogenome.org/gene/300852:TTH_RS10500 ^@ http://purl.uniprot.org/uniprot/P61497 ^@ Cofactor|||Function|||Similarity|||Subunit ^@ Belongs to the DNA photolyase class-1 family.|||Binds 1 FAD per subunit.|||Involved in repair of UV radiation-induced DNA damage. Catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ultraviolet radiation (By similarity).|||Monomer. http://togogenome.org/gene/300852:TTH_RS09760 ^@ http://purl.uniprot.org/uniprot/Q5SH07 ^@ Subcellular Location Annotation ^@ Membrane http://togogenome.org/gene/300852:TTH_RS09090 ^@ http://purl.uniprot.org/uniprot/Q8RQP5 ^@ Cofactor|||Function|||Similarity|||Subunit ^@ Belongs to the IspF family.|||Binds 1 divalent metal cation per subunit.|||Homotrimer.|||Involved in the biosynthesis of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP), two major building blocks of isoprenoid compounds. Catalyzes the conversion of 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDP-ME2P) to 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-CPP) with a corresponding release of cytidine 5-monophosphate (CMP). http://togogenome.org/gene/300852:TTH_RS01315 ^@ http://purl.uniprot.org/uniprot/P60339 ^@ Function|||PTM|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.|||Cytoplasm|||Monomer.|||Phosphorylated on a threonine.|||Protects glycyl-tRNA(Gly) from hydrolysis by E.coli D-aminoacyl-tRNA deacylase (dtd) (PubMed:27224426).|||This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. http://togogenome.org/gene/300852:TTH_RS07300 ^@ http://purl.uniprot.org/uniprot/Q5SID2 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the MnmG family. TrmFO subfamily.|||Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs.|||Cytoplasm http://togogenome.org/gene/300852:TTH_RS04445 ^@ http://purl.uniprot.org/uniprot/P80371 ^@ Function|||Similarity|||Subunit ^@ Belongs to the universal ribosomal protein uS2 family.|||Part of the 30S ribosomal subunit. Contacts protein S8 and may contact the N-terminus of Era.|||Spans the head-body hinge region of the 30S subunit. Is loosely associated with the 30S subunit. http://togogenome.org/gene/300852:TTH_RS06815 ^@ http://purl.uniprot.org/uniprot/Q5SIM2 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the endonuclease V family.|||Cytoplasm|||DNA repair enzyme involved in the repair of deaminated bases. Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA. http://togogenome.org/gene/300852:TTH_RS09340 ^@ http://purl.uniprot.org/uniprot/Q5SH90 ^@ Similarity ^@ Belongs to the UPF0051 (ycf24) family. http://togogenome.org/gene/300852:TTH_RS05960 ^@ http://purl.uniprot.org/uniprot/Q5SJ39 ^@ Cofactor|||Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the methylthiotransferase family. RimO subfamily.|||Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.|||Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein uS12.|||Cytoplasm http://togogenome.org/gene/300852:TTH_RS04380 ^@ http://purl.uniprot.org/uniprot/Q9AJ99 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the RecX family.|||Cytoplasm|||Modulates RecA activity. http://togogenome.org/gene/300852:TTH_RS09030 ^@ http://purl.uniprot.org/uniprot/Q5SHE9 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ An essential GTPase which binds GTP, GDP and possibly (p)ppGpp with moderate affinity, with high nucleotide exchange rates and a fairly low GTP hydrolysis rate. Plays a role in control of the cell cycle, stress response, ribosome biogenesis and in those bacteria that undergo differentiation, in morphogenesis control.|||Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family.|||Cytoplasm|||Monomer. http://togogenome.org/gene/300852:TTH_RS05255 ^@ http://purl.uniprot.org/uniprot/Q5SJH6 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the RNA methyltransferase TrmD family.|||Cytoplasm|||Homodimer.|||Specifically methylates guanosine-37 in various tRNAs. http://togogenome.org/gene/300852:TTH_RS01515 ^@ http://purl.uniprot.org/uniprot/Q5SLK6 ^@ Cofactor|||Miscellaneous|||Similarity ^@ Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.|||Binds 1 FAD per subunit.|||The active site is a redox-active disulfide bond. http://togogenome.org/gene/300852:TTH_RS08980 ^@ http://purl.uniprot.org/uniprot/Q5SHF9 ^@ Similarity|||Subunit ^@ Belongs to the thymidine/pyrimidine-nucleoside phosphorylase family.|||Homodimer. http://togogenome.org/gene/300852:TTH_RS01990 ^@ http://purl.uniprot.org/uniprot/Q5SLB4 ^@ Similarity ^@ Belongs to the bacterial solute-binding protein 1 family. http://togogenome.org/gene/300852:TTH_RS03025 ^@ http://purl.uniprot.org/uniprot/Q5SKR1 ^@ Similarity ^@ Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. http://togogenome.org/gene/300852:TTH_RS09845 ^@ http://purl.uniprot.org/uniprot/Q5SGZ2 ^@ Similarity ^@ Belongs to the acyl-CoA dehydrogenase family. http://togogenome.org/gene/300852:TTH_RS04665 ^@ http://purl.uniprot.org/uniprot/Q5SMF8 ^@ Similarity ^@ Belongs to the glyceraldehyde-3-phosphate dehydrogenase family. http://togogenome.org/gene/300852:TTH_RS08375 ^@ http://purl.uniprot.org/uniprot/Q5SHS5 ^@ Similarity ^@ Belongs to the FPP/GGPP synthase family. http://togogenome.org/gene/300852:TTH_RS10970 ^@ http://purl.uniprot.org/uniprot/Q53WG9 ^@ Function|||Similarity|||Subunit ^@ Belongs to the CRISPR-associated protein Cas6/Cse3/CasE family. Subtype I-E/Ecoli subfamily.|||CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). This enzyme processes pre-crRNA into individual crRNA units, but may not actually undergo enzyme turnover, retaining the crRNA product (PubMed:21572442). Generates a 2',3'-cyclic phosphodiester.|||Probably part of the Cascade ribonucleoprotein complex (Probable). Monomer, retains crRNA after it is processed. http://togogenome.org/gene/300852:TTH_RS01275 ^@ http://purl.uniprot.org/uniprot/Q5SLP9 ^@ Function|||Subunit ^@ Homodimer.|||Plays an important role in DNA replication, recombination and repair. Binds to ssDNA and to an array of partner proteins to recruit them to their sites of action during DNA metabolism. http://togogenome.org/gene/300852:TTH_RS10490 ^@ http://purl.uniprot.org/uniprot/Q53W62 ^@ Activity Regulation|||Domain|||Function|||Similarity|||Subunit ^@ Belongs to the CarA/CarH B12-binding photoregulator family.|||Homotetramer in the dark in the presence of cobalamin. Monomer in the light or in the absence of cobalamin.|||Negative regulator of genes involved in carotenoid biosynthesis.|||Requires cobalamin (vitamin B12) for repressor activity. In the dark, binding of cobalamin to CarH induces its oligomerization, which enhances binding to the DNA and repressor activity. Light causes cobalamin photolysis and disruption of the cobalamin-CarH complex, which decreases interaction with DNA and allows transcription of the target genes.|||The N-terminal region interacts with DNA, and the C-terminal region is involved in oligomerization and binding of cobalamin. http://togogenome.org/gene/300852:TTH_RS10570 ^@ http://purl.uniprot.org/uniprot/Q53W50 ^@ Similarity ^@ Belongs to the acyl-CoA dehydrogenase family. http://togogenome.org/gene/300852:TTH_RS06110 ^@ http://purl.uniprot.org/uniprot/Q5SJ11 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the PlsY family.|||Catalyzes the transfer of an acyl group from acyl-phosphate (acyl-PO(4)) to glycerol-3-phosphate (G3P) to form lysophosphatidic acid (LPA). This enzyme utilizes acyl-phosphate as fatty acyl donor, but not acyl-CoA or acyl-ACP.|||Cell inner membrane|||Probably interacts with PlsX. http://togogenome.org/gene/300852:TTH_RS01495 ^@ http://purl.uniprot.org/uniprot/Q5SLL0 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the lyase 1 family. Argininosuccinate lyase subfamily.|||Cytoplasm http://togogenome.org/gene/300852:TTH_RS09855 ^@ http://purl.uniprot.org/uniprot/P96077 ^@ Function|||PTM|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the prokaryotic/mitochondrial release factor family.|||Cytoplasm|||Interacts with the ribosome (PubMed:16377566).|||Methylated by PrmC. Methylation increases the termination efficiency of RF1 (By similarity).|||Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA (Probable). http://togogenome.org/gene/300852:TTH_RS00030 ^@ http://purl.uniprot.org/uniprot/Q5SMD7 ^@ Cofactor|||Function|||Similarity|||Subunit ^@ Belongs to the transketolase family. DXPS subfamily.|||Binds 1 Mg(2+) ion per subunit.|||Binds 1 thiamine pyrophosphate per subunit.|||Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP).|||Homodimer. http://togogenome.org/gene/300852:TTH_RS03880 ^@ http://purl.uniprot.org/uniprot/Q5SKA1 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the binding-protein-dependent transport system permease family. FecCD subfamily.|||Membrane http://togogenome.org/gene/300852:TTH_RS04440 ^@ http://purl.uniprot.org/uniprot/P43895 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF-Tu.GTP complex up to the GTP hydrolysis stage on the ribosome.|||Belongs to the EF-Ts family.|||Cytoplasm|||Heterotetramer composed of two EF-Ts.EF-Tu dimer complexes. http://togogenome.org/gene/300852:TTH_RS05005 ^@ http://purl.uniprot.org/uniprot/Q5SJM3 ^@ Function|||Similarity ^@ Belongs to the NadC/ModD family.|||Involved in the catabolism of quinolinic acid (QA). http://togogenome.org/gene/300852:TTH_RS06045 ^@ http://purl.uniprot.org/uniprot/Q5SJ24 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the MreD family.|||Membrane http://togogenome.org/gene/300852:TTH_RS10300 ^@ http://purl.uniprot.org/uniprot/Q53WA0 ^@ Caution|||Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the CobD/CbiB family.|||Cell membrane|||Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group.|||Lacks conserved residue(s) required for the propagation of feature annotation.|||Membrane http://togogenome.org/gene/300852:TTH_RS02150 ^@ http://purl.uniprot.org/uniprot/Q5SL81 ^@ Similarity ^@ Belongs to the dGTPase family. Type 2 subfamily. http://togogenome.org/gene/300852:TTH_RS02895 ^@ http://purl.uniprot.org/uniprot/Q5SKT8 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the PlsY family.|||Catalyzes the transfer of an acyl group from acyl-phosphate (acyl-PO(4)) to glycerol-3-phosphate (G3P) to form lysophosphatidic acid (LPA). This enzyme utilizes acyl-phosphate as fatty acyl donor, but not acyl-CoA or acyl-ACP.|||Cell inner membrane|||Probably interacts with PlsX. http://togogenome.org/gene/300852:TTH_RS00905 ^@ http://purl.uniprot.org/uniprot/Q5SLX1 ^@ Function|||Miscellaneous|||Similarity|||Subcellular Location Annotation ^@ Bacitracin is thought to be involved in the inhibition of peptidoglycan synthesis by sequestering undecaprenyl diphosphate, thereby reducing the pool of lipid carrier available.|||Belongs to the UppP family.|||Catalyzes the dephosphorylation of undecaprenyl diphosphate (UPP). Confers resistance to bacitracin.|||Cell inner membrane http://togogenome.org/gene/300852:TTH_RS09295 ^@ http://purl.uniprot.org/uniprot/P74941 ^@ Cofactor|||Domain|||Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the class-II aminoacyl-tRNA synthetase family.|||Binds 1 zinc ion per subunit.|||Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain.|||Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.|||Cytoplasm http://togogenome.org/gene/300852:TTH_RS08320 ^@ http://purl.uniprot.org/uniprot/Q5SHT6 ^@ Similarity ^@ Belongs to the LOG family. http://togogenome.org/gene/300852:TTH_RS10485 ^@ http://purl.uniprot.org/uniprot/Q53W63 ^@ Function|||Subunit ^@ Activates transcription. Positively regulates PcrtB promoter upstream of the crtB operon in a cAMP-independent manner. Regulated genes include genes encoding DNA photolyase, phytoene synthase and cytochrome P450 monooxygenase, which are involved in carotenoid biosynthesis. Positively regulates the light-inducible gene cluster in the megaplasmid in a cAMP-independent manner.|||Homodimer. http://togogenome.org/gene/300852:TTH_RS01805 ^@ http://purl.uniprot.org/uniprot/Q5SLF0 ^@ Similarity ^@ Belongs to the folylpolyglutamate synthase family. http://togogenome.org/gene/300852:TTH_RS03720 ^@ http://purl.uniprot.org/uniprot/Q5SKC7 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the binding-protein-dependent transport system permease family. CysTW subfamily.|||Cell membrane|||Membrane|||Part of the binding-protein-dependent transport system for molybdenum; probably responsible for the translocation of the substrate across the membrane. http://togogenome.org/gene/300852:TTH_RS06660 ^@ http://purl.uniprot.org/uniprot/Q5SIQ4 ^@ Subcellular Location Annotation ^@ Membrane http://togogenome.org/gene/300852:TTH_RS01120 ^@ http://purl.uniprot.org/uniprot/Q5SLS8 ^@ Similarity ^@ Belongs to the asp23 family. http://togogenome.org/gene/300852:TTH_RS01535 ^@ http://purl.uniprot.org/uniprot/Q9F1Q3 ^@ Domain|||Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the RuvA family.|||Binds Holliday junction (HJ) but not linear dsDNA. The RuvA-RuvB complex promotes HJ branch migration in an ATP-dependent manner; ATP-gamma-S does not allow migration. Stimulates the ATPase activity of RuvB in the presence of dsDNA.|||Cytoplasm|||Has three domains with a flexible linker between the domains II and III, and assumes an 'L' shape. Domains I and II are responsible for tetramerization and subsequent octamerization. The flexible linker plus domain III (residues 129-191 in this study) interacts with RuvB.|||Homotetramer; 2 tetramers associate head to head with an empty space between them large enough to hold a Holliday junction. Domain III interacts with RuvB (PubMed:12408833). Electron microscopic images suggest 2 closely interacting RuvA tetramers sandwich the HJ DNA; each RuvA tetramer associates with an RuvB hexamer (PubMed:12408833, PubMed:17981150, PubMed:18068124). Forms 2 complexes with Holliday junction (HJ) DNA which probably have 1 and 2 RuvA tetramers per complex (called complex I and complex II) (PubMed:11245216). Forms a complex with RuvB (Probable) (PubMed:12408833, PubMed:17981150). Each RuvB hexamer is contacted by two RuvA subunits (via domain III) on 2 adjacent RuvB subunits; this complex drives branch migration. In the full resolvosome a probable DNA-RuvA(4)-RuvB(12)-RuvC(2) complex forms which resolves the HJ.|||The RuvA-RuvB-RuvC complex processes Holliday junction (HJ) DNA during genetic recombination and DNA repair, while the RuvA-RuvB complex plays an important role in the rescue of blocked DNA replication forks via replication fork reversal (RFR). RuvA specifically binds to HJ cruciform DNA, conferring on it an open structure. The RuvB hexamer acts as an ATP-dependent pump, pulling dsDNA into and through the RuvAB complex. HJ branch migration allows RuvC to scan DNA until it finds its consensus sequence, where it cleaves and resolves the cruciform DNA. http://togogenome.org/gene/300852:TTH_RS09440 ^@ http://purl.uniprot.org/uniprot/Q5SH69 ^@ Similarity ^@ Belongs to the metallophosphoesterase superfamily. YfcE family. http://togogenome.org/gene/300852:TTH_RS01725 ^@ http://purl.uniprot.org/uniprot/Q5SLG6 ^@ Similarity ^@ Belongs to the methylenetetrahydrofolate reductase family. http://togogenome.org/gene/300852:TTH_RS07435 ^@ http://purl.uniprot.org/uniprot/Q5SIA6 ^@ Similarity ^@ Belongs to the glutamate synthase family. http://togogenome.org/gene/300852:TTH_RS01500 ^@ http://purl.uniprot.org/uniprot/P59846 ^@ Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the argininosuccinate synthase family. Type 1 subfamily.|||Cytoplasm|||Homotetramer. http://togogenome.org/gene/300852:TTH_RS00965 ^@ http://purl.uniprot.org/uniprot/Q5SLV9 ^@ Similarity ^@ Belongs to the 2-oxoacid dehydrogenase family. http://togogenome.org/gene/300852:TTH_RS11045 ^@ http://purl.uniprot.org/uniprot/Q53VX0 ^@ Similarity ^@ Belongs to the ribonucleoside diphosphate reductase large chain family. http://togogenome.org/gene/300852:TTH_RS02090 ^@ http://purl.uniprot.org/uniprot/Q5SL93 ^@ Similarity ^@ Belongs to the zinc-containing alcohol dehydrogenase family. http://togogenome.org/gene/300852:TTH_RS05840 ^@ http://purl.uniprot.org/uniprot/Q5SJ64 ^@ Subcellular Location Annotation ^@ Cytoplasm|||Nucleus http://togogenome.org/gene/300852:TTH_RS06850 ^@ http://purl.uniprot.org/uniprot/Q5SIL5 ^@ Function|||Similarity|||Subunit ^@ Belongs to the type-II 3-dehydroquinase family.|||Catalyzes a trans-dehydration via an enolate intermediate.|||Homododecamer. http://togogenome.org/gene/300852:TTH_RS09710 ^@ http://purl.uniprot.org/uniprot/Q5SH15 ^@ Cofactor|||Function|||Similarity|||Subunit ^@ Belongs to the UPP synthase family.|||Binds 2 magnesium ions per subunit.|||Catalyzes the condensation of isopentenyl diphosphate (IPP) with allylic pyrophosphates generating different type of terpenoids.|||Homodimer. http://togogenome.org/gene/300852:TTH_RS01760 ^@ http://purl.uniprot.org/uniprot/Q5SLF9 ^@ Similarity ^@ Belongs to the UPF0166 family. http://togogenome.org/gene/300852:TTH_RS03625 ^@ http://purl.uniprot.org/uniprot/Q5SKE6 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Cell inner membrane|||Conducts protons, which can be regulated by a proton gradient or by binding of an unfolded protein. Proton conduction requires the large periplasmic domain of the SecD.|||In the C-terminal section; belongs to the SecD/SecF family. SecF subfamily.|||In the N-terminal section; belongs to the SecD/SecF family. SecD subfamily.|||Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. SecDF uses the proton motive force (PMF) to complete protein translocation after the ATP-dependent function of SecA.|||Part of the essential Sec protein translocation apparatus which comprises SecA, SecYEG and auxiliary protein SecDF. Other proteins may also be involved (By similarity). Monomer. http://togogenome.org/gene/300852:TTH_RS01305 ^@ http://purl.uniprot.org/uniprot/P38383 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the SecE/SEC61-gamma family.|||Cell inner membrane|||Component of the Sec protein translocase complex. Heterotrimer consisting of SecY, SecE and SecG subunits. The heterotrimers can form oligomers, although 1 heterotrimer is thought to be able to translocate proteins. Interacts with SecDF, and other proteins may be involved. The channel interacts with SecA via subunit SecY.|||Essential subunit of the protein translocation channel SecYEG. Clamps together the 2 halves of SecY. May contact the channel plug during translocation. http://togogenome.org/gene/300852:TTH_RS00780 ^@ http://purl.uniprot.org/uniprot/Q5SLZ5 ^@ Similarity ^@ Belongs to the pseudouridine synthase RsuA family. http://togogenome.org/gene/300852:TTH_RS03645 ^@ http://purl.uniprot.org/uniprot/P48514 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the NusA family.|||Cytoplasm|||Monomer. Binds directly to the core enzyme of the DNA-dependent RNA polymerase and to nascent RNA.|||Participates in both transcription termination and antitermination. http://togogenome.org/gene/300852:TTH_RS10410 ^@ http://purl.uniprot.org/uniprot/Q5SIU5 ^@ Function|||Similarity ^@ Belongs to the transposase mutator family.|||Required for the transposition of the insertion element. http://togogenome.org/gene/300852:TTH_RS00845 ^@ http://purl.uniprot.org/uniprot/Q5SLY2 ^@ Caution|||Similarity|||Subcellular Location Annotation ^@ Belongs to the class-I aminoacyl-tRNA synthetase family.|||Cytoplasm|||Lacks conserved residue(s) required for the propagation of feature annotation. http://togogenome.org/gene/300852:TTH_RS02710 ^@ http://purl.uniprot.org/uniprot/Q5SKX3 ^@ Cofactor|||Similarity ^@ Belongs to the malic enzymes family.|||Divalent metal cations. Prefers magnesium or manganese. http://togogenome.org/gene/300852:TTH_RS00500 ^@ http://purl.uniprot.org/uniprot/Q56228 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the complex I subunit 4 family.|||Cell inner membrane|||NDH-1 is composed of 15 different subunits, Nqo1 to Nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits Nqo7, Nqo8 and Nqo10 to Nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits Nqo1 to Nqo6, Nqo9 and Nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers.|||NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP. http://togogenome.org/gene/300852:TTH_RS07495 ^@ http://purl.uniprot.org/uniprot/Q5SI94 ^@ Similarity ^@ Belongs to the small heat shock protein (HSP20) family. http://togogenome.org/gene/300852:TTH_RS08940 ^@ http://purl.uniprot.org/uniprot/Q5SHG7 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the NrfD family.|||Membrane http://togogenome.org/gene/300852:TTH_RS07020 ^@ http://purl.uniprot.org/uniprot/Q5SII3 ^@ Caution|||Cofactor|||Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the sugar phosphate cyclases superfamily. Dehydroquinate synthase family.|||Binds 1 divalent metal cation per subunit. Can use either Co(2+) or Zn(2+).|||Catalyzes the conversion of 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) to dehydroquinate (DHQ).|||Cytoplasm|||Lacks conserved residue(s) required for the propagation of feature annotation. http://togogenome.org/gene/300852:TTH_RS02590 ^@ http://purl.uniprot.org/uniprot/Q5SKZ7 ^@ Domain|||Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the complex I Nqo15 family.|||Cell membrane|||Has a similar fold to the mitochondrial iron chaperone frataxin.|||NDH-1 is composed of 15 different subunits, Nqo1 to Nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits Nqo7, Nqo8 and Nqo10 to Nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits Nqo1 to Nqo6, Nqo9 and Nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers. Nqo15 is bound to the side of the complex near the N-terminus of Nqo3, where it interacts with subunits Nqo3, Nqo2, Nqo1, Nqo9 and Nqo4.|||NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP. The Nqo15 subunit has probably a role in complex stabilization, and may be also involved in the storage of iron for iron-sulfur cluster regeneration in the complex. http://togogenome.org/gene/300852:TTH_RS07805 ^@ http://purl.uniprot.org/uniprot/Q5SI38 ^@ Domain|||Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the tRNA(Ile)-lysidine synthase family.|||Cytoplasm|||Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine (By similarity).|||The N-terminal region contains the highly conserved SGGXDS motif, predicted to be a P-loop motif involved in ATP binding. http://togogenome.org/gene/300852:TTH_RS01475 ^@ http://purl.uniprot.org/uniprot/Q5SLL3 ^@ Similarity|||Subunit ^@ Belongs to the CarA family.|||Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. http://togogenome.org/gene/300852:TTH_RS09560 ^@ http://purl.uniprot.org/uniprot/Q5SH44 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the binding-protein-dependent transport system permease family.|||Cell membrane http://togogenome.org/gene/300852:TTH_RS04690 ^@ http://purl.uniprot.org/uniprot/Q5SJU8 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ 3'-5' exoribonuclease that releases 5'-nucleoside monophosphates and is involved in maturation of structured RNAs.|||Belongs to the RNR ribonuclease family. RNase R subfamily.|||Cytoplasm http://togogenome.org/gene/300852:TTH_RS07245 ^@ http://purl.uniprot.org/uniprot/Q5SIE3 ^@ Caution|||Function|||Similarity|||Subunit ^@ Belongs to the dodecin family.|||Dodecin family members from different organisms have non-identical ligand binding specificity.|||Homododecamer; four homotrimers assemble to form a dodecameric hollow sphere with an outer diameter of about 60 Angstroms. Flavin dimers are bound between subunits with a stoichiometry of 6 flavin dimers per dodecamer. Besides, trimeric coenzyme A molecules can be bound between subunits. A dodecamer can bind simultaneously 12 flavin and 12 coenzyme A molecules.|||May function as storage protein that sequesters various flavins and other cofactors, thereby protecting the cell against undesirable reactions mediated by the free cofactors. Binds and sequesters FMN, FAD, lumiflavin and lumichrome, and can also bind coenzyme A. http://togogenome.org/gene/300852:TTH_RS09010 ^@ http://purl.uniprot.org/uniprot/Q5SHF3 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the Iojap/RsfS family.|||Cytoplasm|||Functions as a ribosomal silencing factor. Interacts with ribosomal protein uL14 (rplN), blocking formation of intersubunit bridge B8. Prevents association of the 30S and 50S ribosomal subunits and the formation of functional ribosomes, thus repressing translation.|||Interacts with ribosomal protein uL14 (rplN). http://togogenome.org/gene/300852:TTH_RS09120 ^@ http://purl.uniprot.org/uniprot/Q5SHD4 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the ThiI family.|||Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.|||Cytoplasm http://togogenome.org/gene/300852:TTH_RS07275 ^@ http://purl.uniprot.org/uniprot/Q5SID7 ^@ Disruption Phenotype|||Function|||Subunit ^@ Activates transcription. Positively regulates six promoters upstream of the TTHB186, TTHB147, TTHB178, TTHB159, TTHA0771 and TTHA0176 genes in a cAMP-dependent manner. Regulated genes include clustered regularly interspaced short palindromic repeat (CRISPR) associated (Cas) genes, and the genes encoding a putative transcriptional regulator, a protein containing the exonuclease III-like domain of DNA polymerase, a GCN5-related acetyltransferase homolog, and some T.thermophilus-specific proteins of unknown function. The consensus DNA-binding site of this transcriptional regulator is 5'-(CT)NNG(G/T)(G/T)C(A/C)N(A/T)NNTCACAN(G/C)(G/C)-3' in which N is G, A, T or C.|||Decreased expression of genes downstream of the TTHB186, TTHB147 and TTHB159 genes. Decreased expression of TTHB178, TTHA0771 and TTHA0176 genes.|||Homodimer. http://togogenome.org/gene/300852:TTH_RS04315 ^@ http://purl.uniprot.org/uniprot/Q5SK17 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the 4-toluene sulfonate uptake permease (TSUP) (TC 2.A.102) family.|||Cell membrane|||Membrane http://togogenome.org/gene/300852:TTH_RS05980 ^@ http://purl.uniprot.org/uniprot/Q5SJ35 ^@ Function|||Similarity ^@ Belongs to the fructosamine kinase family.|||Ketoamine kinase that phosphorylates ketoamines, such as erythruloselysine and ribuloselysine, on the third carbon of the sugar moiety to generate ketoamine 3-phosphate (PubMed:17681011). Has higher activity on free lysine (erythruloselysine and ribuloselysine), than on ribuloselysine and erythruloselysine residues on glycated proteins (PubMed:17681011). http://togogenome.org/gene/300852:TTH_RS00960 ^@ http://purl.uniprot.org/uniprot/Q5SLW0 ^@ Similarity ^@ Belongs to the 5-formyltetrahydrofolate cyclo-ligase family. http://togogenome.org/gene/300852:TTH_RS04600 ^@ http://purl.uniprot.org/uniprot/Q5SJW0 ^@ Similarity ^@ Belongs to the acyl-CoA dehydrogenase family. http://togogenome.org/gene/300852:TTH_RS00920 ^@ http://purl.uniprot.org/uniprot/Q5SLW8 ^@ Subcellular Location Annotation ^@ Cytoplasm http://togogenome.org/gene/300852:TTH_RS03805 ^@ http://purl.uniprot.org/uniprot/Q5SKB6 ^@ Similarity ^@ Belongs to the outer membrane factor (OMF) (TC 1.B.17) family. http://togogenome.org/gene/300852:TTH_RS04255 ^@ http://purl.uniprot.org/uniprot/Q5SK28 ^@ Disruption Phenotype|||Function|||Miscellaneous|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the spermidine/spermine synthase family.|||Cells lacking both speB and speE genes show defective growth at 70 degrees Celsius and significantly defective growth at 78 degrees Celsius. They accumulate agmatine and N1-aminopropylagmatine.|||Cytoplasm|||Homotetramer.|||In T.thermophilus, the biosynthetic pathways of spermidine operates via N1-aminopropylagmatine without the production of putrescine.|||Involved in the biosynthesis of polyamines which are thought to support the growth of thermophilic microorganisms under high-temperature conditions. It seems that long-chain and branched-chain of polyamines effectively stabilize DNA and RNA, respectively. Catalyzes the irreversible transfer of a propylamine group from the amino donor S-adenosylmethioninamine (decarboxy-AdoMet) to agmatine to yield N1-aminopropylagmatine. An efficient aminopropyltransferase activity has been also observed with norspermidine which produces thermine, and spermidine which produces spermine and thermospermine. The aminopropyl activity with homospermidine, mitsubishine, thermine, 1,3-diaminopropane, putrescine (1,4-diaminobutane), spermine and caldopentamine are very low. The reaction involves a nucleophilic attack on the C-3 methylene of the propylamine moiety adjacent to the positively charged sulfur of decarboxy-AdoMet. http://togogenome.org/gene/300852:TTH_RS07060 ^@ http://purl.uniprot.org/uniprot/Q5SIH5 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the GHMP kinase family. Homoserine kinase subfamily.|||Catalyzes the ATP-dependent phosphorylation of L-homoserine to L-homoserine phosphate.|||Cytoplasm http://togogenome.org/gene/300852:TTH_RS02640 ^@ http://purl.uniprot.org/uniprot/Q5SKY7 ^@ Similarity ^@ Belongs to the malate synthase family. http://togogenome.org/gene/300852:TTH_RS05920 ^@ http://purl.uniprot.org/uniprot/Q5SJ47 ^@ Cofactor|||Function|||Similarity|||Subunit ^@ Belongs to the RecJ family.|||Monomer. Intact protein binds ss- but not dsDNA.|||Requires a divalent cation; Mg(2+) > Mn(2+) > Co(2+). Structures with 1 Mg(2+), or 1 or 2 Mn(2+) were determined; the authors conclude 2 metal ions are required for activity.|||Single-stranded-DNA-specific exonuclease acting in a 5' to 3' direction; has no detectable activity on ssRNA. http://togogenome.org/gene/300852:TTH_RS11095 ^@ http://purl.uniprot.org/uniprot/Q53VW0 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the binding-protein-dependent transport system permease family. FecCD subfamily.|||Membrane http://togogenome.org/gene/300852:TTH_RS09335 ^@ http://purl.uniprot.org/uniprot/Q5SH91 ^@ Similarity ^@ Belongs to the UPF0051 (ycf24) family. http://togogenome.org/gene/300852:TTH_RS08040 ^@ http://purl.uniprot.org/uniprot/Q5SHZ2 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the PTH family.|||Cytoplasm|||Monomer.|||The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis. http://togogenome.org/gene/300852:TTH_RS08500 ^@ http://purl.uniprot.org/uniprot/Q5SHQ0 ^@ Caution|||Function|||Similarity|||Subunit ^@ Belongs to the universal ribosomal protein uL5 family.|||In PubMed:11154066 there is uncertainty about the number of Arg residues at position 10 and whether position 25 is Pro or Tyr.|||Part of the 50S ribosomal subunit; part of the 5S rRNA/uL5/uL18/bL25 (TL7) subcomplex; has also been isolated as a complex with 5S rRNA, bL25 (TL7) and DNA binding protein II. Forms a bridge to the 30S subunit in the 70S ribosome, contacting protein uS13; this bridge is straddled by the 5S rRNA. Contacts the P site tRNA.|||This is one of the proteins that bind and probably mediate the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance. In the 70S ribosome it contacts protein S13 of the 30S subunit (forming bridge B1b) connecting the head of the 30S subunit to the top of the 50S subunit. The bridge itself contacts the P site tRNA and is implicated in movement during ribosome translocation. Also contacts the P site tRNA independently of the intersubunit bridge; the 5S rRNA and some of its associated proteins might help stabilize positioning of ribosome-bound tRNAs. http://togogenome.org/gene/300852:TTH_RS03285 ^@ http://purl.uniprot.org/uniprot/Q5SKL5 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the TatA/E family.|||Cell inner membrane|||Forms a complex with TatC.|||Part of the twin-arginine translocation (Tat) system that transports large folded proteins containing a characteristic twin-arginine motif in their signal peptide across membranes. TatA could form the protein-conducting channel of the Tat system. http://togogenome.org/gene/300852:TTH_RS03060 ^@ http://purl.uniprot.org/uniprot/Q5SKQ4 ^@ Subcellular Location Annotation ^@ Membrane http://togogenome.org/gene/300852:TTH_RS01705 ^@ http://purl.uniprot.org/uniprot/Q5SLH0 ^@ Caution|||Function|||Similarity|||Subunit ^@ Belongs to the adenylate cyclase family. DacA/CdaA subfamily.|||Catalyzes the condensation of 2 ATP molecules into cyclic di-AMP (c-di-AMP), a second messenger used to regulate differing processes in different bacteria.|||Lacks conserved residue(s) required for the propagation of feature annotation.|||Probably a homodimer. http://togogenome.org/gene/300852:TTH_RS06695 ^@ http://purl.uniprot.org/uniprot/Q5SIP7 ^@ Activity Regulation|||Cofactor|||Function|||Similarity|||Subunit ^@ Belongs to the class II aldolase/RraA-like family.|||Catalyzes the aldol cleavage of 4-hydroxy-4-methyl-2-oxoglutarate (HMG) into 2 molecules of pyruvate. Also contains a secondary oxaloacetate (OAA) decarboxylase activity due to the common pyruvate enolate transition state formed following C-C bond cleavage in the retro-aldol and decarboxylation reactions.|||Competitively inhibited by oxalate, a pyruvate enolate analog.|||Divalent metal cation. Has preference for nickel and cobalt ions for the HMG aldolase activity. Has preference for zinc ions for the OAA decarboxylase activity.|||Homotrimer. http://togogenome.org/gene/300852:TTH_RS10640 ^@ http://purl.uniprot.org/uniprot/Q5SIU5 ^@ Function|||Similarity ^@ Belongs to the transposase mutator family.|||Required for the transposition of the insertion element. http://togogenome.org/gene/300852:TTH_RS09155 ^@ http://purl.uniprot.org/uniprot/Q5SHC7 ^@ Similarity ^@ Belongs to the pterin-4-alpha-carbinolamine dehydratase family. http://togogenome.org/gene/300852:TTH_RS03840 ^@ http://purl.uniprot.org/uniprot/Q5SKA9 ^@ Similarity ^@ Belongs to the iron-sulfur dependent L-serine dehydratase family. http://togogenome.org/gene/300852:TTH_RS05110 ^@ http://purl.uniprot.org/uniprot/Q5SJK2 ^@ Similarity ^@ Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family. http://togogenome.org/gene/300852:TTH_RS02525 ^@ http://purl.uniprot.org/uniprot/Q5SL08 ^@ Similarity ^@ Belongs to the metallophosphoesterase superfamily. YfcE family. http://togogenome.org/gene/300852:TTH_RS06575 ^@ http://purl.uniprot.org/uniprot/P23395 ^@ Cofactor|||Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2A subfamily.|||Binds 1 zinc ion per subunit.|||Cytoplasm|||Homodimer.|||Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation. http://togogenome.org/gene/300852:TTH_RS01125 ^@ http://purl.uniprot.org/uniprot/Q5SLS7 ^@ Similarity ^@ Belongs to the AlaDH/PNT family. http://togogenome.org/gene/300852:TTH_RS06135 ^@ http://purl.uniprot.org/uniprot/Q5SJ06 ^@ Similarity ^@ Belongs to the alpha-IPM synthase/homocitrate synthase family. http://togogenome.org/gene/300852:TTH_RS05475 ^@ http://purl.uniprot.org/uniprot/Q5SJD9 ^@ Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the MraZ family.|||Forms oligomers.|||nucleoid http://togogenome.org/gene/300852:TTH_RS04865 ^@ http://purl.uniprot.org/uniprot/Q5SJQ1 ^@ Caution|||Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the DapA family.|||Catalyzes the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to 4-hydroxy-tetrahydrodipicolinate (HTPA).|||Cytoplasm|||Homotetramer; dimer of dimers.|||Was originally thought to be a dihydrodipicolinate synthase (DHDPS), catalyzing the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to dihydrodipicolinate (DHDP). However, it was shown in E.coli that the product of the enzymatic reaction is not dihydrodipicolinate but in fact (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinic acid (HTPA), and that the consecutive dehydration reaction leading to DHDP is not spontaneous but catalyzed by DapB. http://togogenome.org/gene/300852:TTH_RS07695 ^@ http://purl.uniprot.org/uniprot/Q5SMH7 ^@ Cofactor|||Function|||Similarity ^@ Binds 1 Mg(2+) ion per subunit.|||Binds 1 [4Fe-4S] cluster per subunit.|||Catalyzes the formation of phosphoribosylamine from phosphoribosylpyrophosphate (PRPP) and glutamine.|||In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family. http://togogenome.org/gene/300852:TTH_RS01290 ^@ http://purl.uniprot.org/uniprot/Q5SLP7 ^@ Function|||Similarity|||Subunit ^@ Belongs to the universal ribosomal protein uL1 family.|||Directly binds to 23S rRNA. Forms what is known as the L1 stalk, which protrudes beyond the 70S ribosome surface. The stalk is preferentially stabilized in 70S versus 50S crystals. Interacts with the E site tRNA, blocking the exit path. This blockage implies that this section of the ribosome must be able to move to release the deacetylated tRNA.|||Part of the 50S ribosomal subunit.|||Protein L1 is also a translational repressor protein, it controls the translation of the L11 operon by binding to its mRNA. http://togogenome.org/gene/300852:TTH_RS10725 ^@ http://purl.uniprot.org/uniprot/Q53W23 ^@ Similarity ^@ Belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family. http://togogenome.org/gene/300852:TTH_RS09320 ^@ http://purl.uniprot.org/uniprot/Q5SH94 ^@ Cofactor ^@ Can also use Mn(2+) ion. http://togogenome.org/gene/300852:TTH_RS03815 ^@ http://purl.uniprot.org/uniprot/Q5SKB4 ^@ Subcellular Location Annotation ^@ Membrane http://togogenome.org/gene/300852:TTH_RS01555 ^@ http://purl.uniprot.org/uniprot/Q5SLJ8 ^@ Similarity ^@ Belongs to the universal stress protein A family. http://togogenome.org/gene/300852:TTH_RS01625 ^@ http://purl.uniprot.org/uniprot/Q5SLI3 ^@ Function|||Miscellaneous|||Similarity|||Subcellular Location Annotation ^@ Carbon 2 of the heme B porphyrin ring is defined according to the Fischer nomenclature.|||Cell inner membrane|||Converts heme B (protoheme IX) to heme O by substitution of the vinyl group on carbon 2 of heme B porphyrin ring with a hydroxyethyl farnesyl side group.|||In the C-terminal section; belongs to the UbiA prenyltransferase family. Protoheme IX farnesyltransferase subfamily.|||In the N-terminal section; belongs to the COX15/CtaA family. http://togogenome.org/gene/300852:TTH_RS07315 ^@ http://purl.uniprot.org/uniprot/Q5SIC9 ^@ Similarity|||Subcellular Location Annotation ^@ Cytoplasm|||In the C-terminal section; belongs to the PRA-PH family.|||In the N-terminal section; belongs to the PRA-CH family. http://togogenome.org/gene/300852:TTH_RS02910 ^@ http://purl.uniprot.org/uniprot/Q5SKT5 ^@ Function|||Miscellaneous|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.|||Cytoplasm|||Homotetramer.|||Involved in the TCA cycle. Catalyzes the stereospecific interconversion of fumarate to L-malate.|||There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors. http://togogenome.org/gene/300852:TTH_RS07630 ^@ http://purl.uniprot.org/uniprot/Q5SI67 ^@ Function|||Similarity ^@ Belongs to the uroporphyrinogen-III synthase family.|||Catalyzes cyclization of the linear tetrapyrrole, hydroxymethylbilane, to the macrocyclic uroporphyrinogen III. http://togogenome.org/gene/300852:TTH_RS08355 ^@ http://purl.uniprot.org/uniprot/Q5SHS9 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the binding-protein-dependent transport system permease family. MalFG subfamily.|||Cell membrane|||Part of the ABC transporter complex MalEFGK involved in maltose/maltodextrin import. Probably responsible for the translocation of the substrate across the membrane. http://togogenome.org/gene/300852:TTH_RS01130 ^@ http://purl.uniprot.org/uniprot/Q5SLS6 ^@ Function|||Similarity ^@ Belongs to the pseudouridine synthase TruB family. Type 1 subfamily.|||Responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs. http://togogenome.org/gene/300852:TTH_RS08385 ^@ http://purl.uniprot.org/uniprot/Q5SHS3 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the transcriptional regulatory Rex family.|||Cytoplasm|||Homodimer.|||Modulates transcription in response to changes in cellular NADH/NAD(+) redox state. http://togogenome.org/gene/300852:TTH_RS10340 ^@ http://purl.uniprot.org/uniprot/Q53W92 ^@ Cofactor|||Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the SurE nucleotidase family.|||Binds 1 divalent metal cation per subunit.|||Cytoplasm|||Nucleotidase that shows phosphatase activity on nucleoside 5'-monophosphates. http://togogenome.org/gene/300852:TTH_RS02265 ^@ http://purl.uniprot.org/uniprot/Q5SL58 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the DsbB family. BdbC subfamily.|||Membrane http://togogenome.org/gene/300852:TTH_RS04670 ^@ http://purl.uniprot.org/uniprot/P09403 ^@ Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the phosphoglycerate kinase family.|||Cytoplasm|||Monomer. http://togogenome.org/gene/300852:TTH_RS03160 ^@ http://purl.uniprot.org/uniprot/Q5SKN5 ^@ Function|||Similarity|||Subunit ^@ Associates with stalled 50S ribosomal subunits.|||Belongs to the NEMF family.|||Part of the ribosome quality control system (RQC). Recruits Ala-charged tRNA and directs the elongation of stalled nascent chains on 50S ribosomal subunits, leading to non-templated C-terminal Ala extensions (Ala tail). The Ala tail promotes nascent chain degradation. May add between 1 and at least 8 Ala residues. Binds to stalled 50S ribosomal subunits. http://togogenome.org/gene/300852:TTH_RS08660 ^@ http://purl.uniprot.org/uniprot/Q5SHM0 ^@ Cofactor ^@ Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine. http://togogenome.org/gene/300852:TTH_RS10870 ^@ http://purl.uniprot.org/uniprot/Q53VZ7 ^@ Caution|||Domain|||Function|||Miscellaneous|||Similarity|||Subunit ^@ Both the DNA unwinding and positive supercoiling activities require the cooperation of both domains. The cooperative action between the helicase-like and the topoisomerase domains is specific. The helicase-like domain probably does not directly unwind DNA but acts more likely by driving ATP-dependent conformational changes within the whole enzyme, functioning more like a protein motor. The 'latch' region of the N-terminal domain plays a regulatory role in the enzyme, repressing topoisomerase activity in the absence of ATP and therefore preventing the enzyme from acting as an ATP-independent relaxing enzyme; it also helps to coordinate nucleotide hydrolysis by the ATPase domain with the supercoiling activity of the topoisomerase domain.|||In the C-terminal section; belongs to the prokaryotic type I/III topoisomerase family.|||In the N-terminal section; belongs to the DEAD box helicase family. DDVD subfamily.|||Lacks conserved residue(s) required for the propagation of feature annotation.|||Modifies the topological state of DNA by introducing positive supercoils in an ATP-dependent process. It cleaves transiently a single DNA strand and remains covalently bound to the 5' DNA end through a tyrosine residue. May be involved in rewinding the DNA strands in the regions of the chromosome that have opened up to allow transcription or replication.|||Monomer.|||This enzyme is the only unique feature of hyperthermophilic bacteria/archaea discovered so far. It appears to be essential for adaptation to life at high temperatures. http://togogenome.org/gene/300852:TTH_RS06090 ^@ http://purl.uniprot.org/uniprot/Q5SJ15 ^@ Caution|||Similarity|||Subcellular Location Annotation ^@ Belongs to the aspartate/ornithine carbamoyltransferase superfamily. OTCase family.|||Cytoplasm|||Lacks conserved residue(s) required for the propagation of feature annotation. http://togogenome.org/gene/300852:TTH_RS06250 ^@ http://purl.uniprot.org/uniprot/Q5SIY4 ^@ Cofactor|||Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily.|||Binds 1 Mg(2+) or Mn(2+) ion per subunit.|||Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate.|||Cytoplasm|||Homodimer. http://togogenome.org/gene/300852:TTH_RS04965 ^@ http://purl.uniprot.org/uniprot/Q5SJN1 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the binding-protein-dependent transport system permease family.|||Cell membrane http://togogenome.org/gene/300852:TTH_RS01375 ^@ http://purl.uniprot.org/uniprot/Q5SLN4 ^@ Function|||Similarity ^@ Belongs to the PstS family.|||Involved in the system for phosphate transport across the cytoplasmic membrane. http://togogenome.org/gene/300852:TTH_RS09330 ^@ http://purl.uniprot.org/uniprot/Q5SH92 ^@ Similarity ^@ Belongs to the ABC transporter superfamily. Ycf16 family. http://togogenome.org/gene/300852:TTH_RS04415 ^@ http://purl.uniprot.org/uniprot/Q5SJZ7 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the peptidase M50B family.|||Membrane http://togogenome.org/gene/300852:TTH_RS08540 ^@ http://purl.uniprot.org/uniprot/Q5SHP2 ^@ Function|||Similarity|||Subunit ^@ Belongs to the universal ribosomal protein uS19 family.|||Located at the top of the head of the 30S subunit, extending towards the 50S subunit, which it may contact in the 70S complex. Contacts several RNA helices of the 16S rRNA.|||Part of the 30S ribosomal subunit. Forms a loose dimer with protein S13. http://togogenome.org/gene/300852:TTH_RS07995 ^@ http://purl.uniprot.org/uniprot/Q5SI01 ^@ Cofactor ^@ Binds 1 FAD per subunit. http://togogenome.org/gene/300852:TTH_RS03775 ^@ http://purl.uniprot.org/uniprot/Q5SMF6 ^@ Cofactor|||Function|||Similarity|||Subunit ^@ Belongs to the aconitase/IPM isomerase family.|||Binds 1 [4Fe-4S] cluster per subunit.|||Involved in the catabolism of short chain fatty acids (SCFA) via the tricarboxylic acid (TCA)(acetyl degradation route) and probably the 2-methylcitrate cycle I (propionate degradation route). Catalyzes the reversible isomerization of citrate to isocitrate via cis-aconitate. Also able to catalyze the hydration of cis-homoaconitate to yield (R)-homocitrate, but with a lower efficiency (PubMed:23106124). Could catalyze the hydration of 2-methyl-cis-aconitate to yield (2R,3S)-2-methylisocitrate. The apo form of AcnA functions as a RNA-binding regulatory protein (By similarity).|||Monomer. http://togogenome.org/gene/300852:TTH_RS06195 ^@ http://purl.uniprot.org/uniprot/Q5SIZ3 ^@ Subcellular Location Annotation ^@ Cell outer membrane|||Periplasm http://togogenome.org/gene/300852:TTH_RS01920 ^@ http://purl.uniprot.org/uniprot/Q5SLC6 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the AIR synthase family.|||Cytoplasm http://togogenome.org/gene/300852:TTH_RS03120 ^@ http://purl.uniprot.org/uniprot/Q5SKP2 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the uroporphyrinogen decarboxylase family.|||Catalyzes the decarboxylation of four acetate groups of uroporphyrinogen-III to yield coproporphyrinogen-III.|||Cytoplasm|||Homodimer. http://togogenome.org/gene/300852:TTH_RS07485 ^@ http://purl.uniprot.org/uniprot/Q5SI96 ^@ Subcellular Location Annotation ^@ Membrane http://togogenome.org/gene/300852:TTH_RS01280 ^@ http://purl.uniprot.org/uniprot/Q5SLP8 ^@ Function|||Similarity|||Subunit ^@ Belongs to the bacterial ribosomal protein bS6 family.|||Located on the outer edge of the platform on the body of the 30S subunit.|||Part of the 30S ribosomal subunit. Forms a tight heterodimer with protein bS18. May make transient contacts with protein uL2 of the 50S subunit during translation. http://togogenome.org/gene/300852:TTH_RS05640 ^@ http://purl.uniprot.org/uniprot/Q5SJA6 ^@ Subcellular Location Annotation ^@ Membrane http://togogenome.org/gene/300852:TTH_RS09675 ^@ http://purl.uniprot.org/uniprot/Q5SH22 ^@ Function|||Miscellaneous|||PTM ^@ Carrier protein that bears the covalently bound substrates for lysine biosynthesis; the bound alpha-aminoadipate (AAA) is sequentially converted to L-lysine.|||Formation of an isopeptide bond between the gamma-carboxyl group of the C-terminal glutamate and the amino group of alpha-aminoadipate (AAA) is catalyzed by LysX. The bound AAA is then converted to L-lysine in a series of reactions catalyzed by LysZ, LysY and LysJ. Release of the product L-lysine is catalyzed by LysK (PubMed:19620981).|||The purified protein is red, and its absorption spectrum suggests that it binds iron ions, possibly via the predicted zinc finger domain (PubMed:19620981). In contrast, the ortholog from Sulfolobus clearly binds zinc ions via its zinc finger domain, as shown by X-ray crystallography. http://togogenome.org/gene/300852:TTH_RS09185 ^@ http://purl.uniprot.org/uniprot/Q5SHC1 ^@ Similarity ^@ Belongs to the peptidase S33 family. http://togogenome.org/gene/300852:TTH_RS07725 ^@ http://purl.uniprot.org/uniprot/Q5SI54 ^@ Similarity ^@ Belongs to the HMG-CoA lyase family. http://togogenome.org/gene/300852:TTH_RS00725 ^@ http://purl.uniprot.org/uniprot/Q5SM06 ^@ Similarity ^@ Belongs to the RutC family. http://togogenome.org/gene/300852:TTH_RS03445 ^@ http://purl.uniprot.org/uniprot/Q5SKI3 ^@ Similarity ^@ Belongs to the short-chain dehydrogenases/reductases (SDR) family. http://togogenome.org/gene/300852:TTH_RS09460 ^@ http://purl.uniprot.org/uniprot/Q5SH65 ^@ Similarity ^@ Belongs to the peptidase S8 family. http://togogenome.org/gene/300852:TTH_RS04610 ^@ http://purl.uniprot.org/uniprot/Q5SJV8 ^@ Function|||PTM|||Similarity|||Subcellular Location Annotation ^@ Belongs to the HSP33 family.|||Cytoplasm|||Redox regulated molecular chaperone. Protects both thermally unfolding and oxidatively damaged proteins from irreversible aggregation. Plays an important role in the bacterial defense system toward oxidative stress.|||Under oxidizing conditions two disulfide bonds are formed involving the reactive cysteines. Under reducing conditions zinc is bound to the reactive cysteines and the protein is inactive. http://togogenome.org/gene/300852:TTH_RS05890 ^@ http://purl.uniprot.org/uniprot/Q5SJ53 ^@ Caution ^@ Lacks conserved residue(s) required for the propagation of feature annotation. http://togogenome.org/gene/300852:TTH_RS05405 ^@ http://purl.uniprot.org/uniprot/Q5SJF2 ^@ Cofactor|||Function|||Similarity ^@ Binds 1 zinc ion per subunit.|||Binds 2 divalent metal cations per subunit. Magnesium or manganese.|||Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate.|||Catalyzes the conversion of GTP to 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate.|||In the C-terminal section; belongs to the GTP cyclohydrolase II family.|||In the N-terminal section; belongs to the DHBP synthase family. http://togogenome.org/gene/300852:TTH_RS03485 ^@ http://purl.uniprot.org/uniprot/Q5SKH5 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the 4-toluene sulfonate uptake permease (TSUP) (TC 2.A.102) family.|||Cell membrane|||Membrane http://togogenome.org/gene/300852:TTH_RS01530 ^@ http://purl.uniprot.org/uniprot/Q5SLK3 ^@ Similarity ^@ Belongs to the enoyl-CoA hydratase/isomerase family. http://togogenome.org/gene/300852:TTH_RS03570 ^@ http://purl.uniprot.org/uniprot/Q5SKF8 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the binding-protein-dependent transport system permease family.|||Cell membrane http://togogenome.org/gene/300852:TTH_RS05240 ^@ http://purl.uniprot.org/uniprot/Q5SJH9 ^@ Similarity ^@ Belongs to the iron-sulfur dependent L-serine dehydratase family. http://togogenome.org/gene/300852:TTH_RS07070 ^@ http://purl.uniprot.org/uniprot/Q5SIH3 ^@ Function|||Similarity|||Subunit ^@ Belongs to the bacterial ribosomal protein bTHX family.|||Binds at the top of the head of the 30S subunit. It stabilizes a number of different RNA elements and thus is important for subunit structure.|||Part of the 30S ribosomal subunit. http://togogenome.org/gene/300852:TTH_RS09415 ^@ http://purl.uniprot.org/uniprot/Q5SH74 ^@ Subcellular Location Annotation ^@ Membrane http://togogenome.org/gene/300852:TTH_RS05360 ^@ http://purl.uniprot.org/uniprot/Q5SJF5 ^@ Similarity ^@ Belongs to the IMPACT family. http://togogenome.org/gene/300852:TTH_RS06845 ^@ http://purl.uniprot.org/uniprot/Q5SIL6 ^@ Similarity ^@ Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family. http://togogenome.org/gene/300852:TTH_RS05260 ^@ http://purl.uniprot.org/uniprot/Q5SJH5 ^@ Domain|||Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ An accessory protein needed during the final step in the assembly of 30S ribosomal subunit, possibly for assembly of the head region. Essential for efficient processing of 16S rRNA. May be needed both before and after RbfA during the maturation of 16S rRNA. It has affinity for free ribosomal 30S subunits but not for 70S ribosomes.|||Belongs to the RimM family.|||Binds ribosomal protein uS19.|||Cytoplasm|||The isolated N-terminus (residues 1-80) forms a closed, 6-stranded beta barrel; it probably has the same form in full-length protein (PubMed:17616598). The PRC barrel domain binds ribosomal protein uS19 (By similarity). http://togogenome.org/gene/300852:TTH_RS00515 ^@ http://purl.uniprot.org/uniprot/Q5SM44 ^@ Similarity ^@ Belongs to the peptidase S1C family. http://togogenome.org/gene/300852:TTH_RS05585 ^@ http://purl.uniprot.org/uniprot/P26996 ^@ Function|||Similarity ^@ Belongs to the NAD-dependent DNA ligase family. LigA subfamily.|||DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA. http://togogenome.org/gene/300852:TTH_RS00465 ^@ http://purl.uniprot.org/uniprot/Q56222 ^@ Cofactor|||Domain|||Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the complex I 51 kDa subunit family.|||Binds 1 FMN per subunit.|||Binds 1 [4Fe-4S] cluster per subunit. This [4Fe-4S] cluster is referred to as N3.|||Cell membrane|||NDH-1 is composed of 15 different subunits, Nqo1 to Nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits Nqo7, Nqo8 and Nqo10 to Nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits Nqo1 to Nqo6, Nqo9 and Nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers.|||NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP. The Nqo1 subunit contains the NADH-binding site and the primary electron acceptor FMN.|||The subunit can be separated roughly into four domains: an N-terminal domain (residues 7 to 72) ending with a glycine-rich loop, followed by a Rossman-fold domain (73 to 240), a ubiquitin-like domain (241 to 335) and a C-terminal four-helical bundle containing cluster N3 (336 to 438). http://togogenome.org/gene/300852:TTH_RS01400 ^@ http://purl.uniprot.org/uniprot/Q5SLM9 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the RecF family.|||Cytoplasm|||The RecF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP. http://togogenome.org/gene/300852:TTH_RS00450 ^@ http://purl.uniprot.org/uniprot/Q56219 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the complex I 30 kDa subunit family.|||Cell membrane|||NDH-1 is composed of 15 different subunits, Nqo1 to Nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits Nqo7, Nqo8 and Nqo10 to Nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits Nqo1 to Nqo6, Nqo9 and Nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers. The N-terminal domain of this subunit (residues 1 to 153) wraps around Nqo4 on one side, and the subunit interacts also with Nqo9 via a two-stranded beta sheet (residues 154 to 171) and with Nqo3 via an extended C-terminal loop (residues 172 to 196).|||NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP. The Nqo5 subunit may be involved in the stabilization of the complex. http://togogenome.org/gene/300852:TTH_RS08715 ^@ http://purl.uniprot.org/uniprot/Q5SHL0 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.|||Cell membrane http://togogenome.org/gene/300852:TTH_RS01085 ^@ http://purl.uniprot.org/uniprot/Q5SLT5 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the PNT beta subunit family.|||Cell inner membrane|||Membrane|||The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane. http://togogenome.org/gene/300852:TTH_RS02015 ^@ http://purl.uniprot.org/uniprot/Q5SLA9 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the Lgt family.|||Catalyzes the transfer of the diacylglyceryl group from phosphatidylglycerol to the sulfhydryl group of the N-terminal cysteine of a prolipoprotein, the first step in the formation of mature lipoproteins.|||Cell inner membrane http://togogenome.org/gene/300852:TTH_RS00675 ^@ http://purl.uniprot.org/uniprot/Q5SM15 ^@ Domain|||Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the ATP phosphoribosyltransferase family. Short subfamily.|||Catalyzes the condensation of ATP and 5-phosphoribose 1-diphosphate to form N'-(5'-phosphoribosyl)-ATP (PR-ATP). Has a crucial role in the pathway because the rate of histidine biosynthesis seems to be controlled primarily by regulation of HisG enzymatic activity.|||Cytoplasm|||Heteromultimer composed of HisG and HisZ subunits.|||Lacks the C-terminal regulatory region which is replaced by HisZ. http://togogenome.org/gene/300852:TTH_RS04745 ^@ http://purl.uniprot.org/uniprot/Q5SJS6 ^@ Similarity ^@ Belongs to the HicA mRNA interferase family. http://togogenome.org/gene/300852:TTH_RS06345 ^@ http://purl.uniprot.org/uniprot/Q5SIW3 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the SecA family.|||Cell inner membrane|||Cytoplasm|||Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. Has a central role in coupling the hydrolysis of ATP to the transfer of proteins into and across the cell membrane, serving as an ATP-driven molecular motor driving the stepwise translocation of polypeptide chains across the membrane.|||Part of the essential Sec protein translocation apparatus which comprises SecA, SecYEG and auxiliary proteins SecDF. Other proteins may also be involved. Monomer and homodimer. http://togogenome.org/gene/300852:TTH_RS08535 ^@ http://purl.uniprot.org/uniprot/Q5SHP3 ^@ Function|||Similarity|||Subunit ^@ Belongs to the universal ribosomal protein uL22 family.|||Part of the 50S ribosomal subunit.|||The globular domain of the protein is one of the proteins that surrounds the polypeptide exit tunnel on the outside of the subunit, while an extended beta-hairpin is found that penetrates into the center of the 70S ribosome. This extension seems to form part of the wall of the exit tunnel.|||This protein binds specifically to 23S rRNA; its binding is stimulated by other ribosomal proteins, e.g. L4, L17, and L20. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome (By similarity). http://togogenome.org/gene/300852:TTH_RS00095 ^@ http://purl.uniprot.org/uniprot/Q5SMC7 ^@ Function|||Similarity ^@ Belongs to the Dus family. DusA subfamily.|||Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines. Specifically modifies U20 and U20a in tRNAs. http://togogenome.org/gene/300852:TTH_RS07410 ^@ http://purl.uniprot.org/uniprot/P80374 ^@ Function|||Similarity|||Subunit ^@ Belongs to the universal ribosomal protein uS9 family.|||Part of the 30S ribosomal subunit. Contacts proteins S7 and S10.|||Part of the top of the head of the 30S subunit. The C-terminal region penetrates the head emerging in the P-site where it contacts tRNA. http://togogenome.org/gene/300852:TTH_RS02485 ^@ http://purl.uniprot.org/uniprot/Q5SL14 ^@ Function|||Similarity ^@ Belongs to the trehalose phosphatase family.|||Removes the phosphate from trehalose 6-phosphate to produce free trehalose. http://togogenome.org/gene/300852:TTH_RS07595 ^@ http://purl.uniprot.org/uniprot/Q5SI74 ^@ Function|||Similarity ^@ Belongs to the PEP-utilizing enzyme family.|||Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate. http://togogenome.org/gene/300852:TTH_RS05295 ^@ http://purl.uniprot.org/uniprot/Q5SJG8 ^@ Subcellular Location Annotation ^@ Membrane http://togogenome.org/gene/300852:TTH_RS06020 ^@ http://purl.uniprot.org/uniprot/Q5SJ29 ^@ Function|||Similarity ^@ ATPase that binds to both the 70S ribosome and the 50S ribosomal subunit in a nucleotide-independent manner.|||Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family. YchF/OLA1 subfamily. http://togogenome.org/gene/300852:TTH_RS07185 ^@ http://purl.uniprot.org/uniprot/Q5SME7 ^@ Cofactor|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the SoxA family.|||Binds 2 heme groups per subunit.|||Heterodimer of SoxA and SoxX.|||Periplasm http://togogenome.org/gene/300852:TTH_RS02050 ^@ http://purl.uniprot.org/uniprot/Q5SLA1 ^@ Similarity ^@ Belongs to the methylmalonyl-CoA epimerase family. http://togogenome.org/gene/300852:TTH_RS00900 ^@ http://purl.uniprot.org/uniprot/Q5SLX2 ^@ Function|||Similarity ^@ Belongs to the IspD/TarI cytidylyltransferase family. IspD subfamily.|||Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP). http://togogenome.org/gene/300852:TTH_RS08135 ^@ http://purl.uniprot.org/uniprot/Q5SHX3 ^@ Function|||Similarity ^@ Belongs to the thymidylate kinase family.|||Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis. http://togogenome.org/gene/300852:TTH_RS03705 ^@ http://purl.uniprot.org/uniprot/P56194 ^@ Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the class-II aminoacyl-tRNA synthetase family.|||Cytoplasm|||Homodimer. http://togogenome.org/gene/300852:TTH_RS08680 ^@ http://purl.uniprot.org/uniprot/Q5SHL7 ^@ Similarity ^@ Belongs to the UPF0047 family. http://togogenome.org/gene/300852:TTH_RS10510 ^@ http://purl.uniprot.org/uniprot/Q53W58 ^@ Subcellular Location Annotation ^@ Membrane http://togogenome.org/gene/300852:TTH_RS07350 ^@ http://purl.uniprot.org/uniprot/Q5SIC2 ^@ Function|||Induction|||Miscellaneous|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Aspartate mischarged on tRNA(Asn) is then converted into asparagine by the tRNA-dependent amidotransferase GatCAB.|||Aspartyl-tRNA synthetase with relaxed tRNA specificity since it is able to aspartylate not only its cognate tRNA(Asp) but also tRNA(Asn) with similar efficiencies. Reaction proceeds in two steps: L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp/Asn).|||Belongs to the class-II aminoacyl-tRNA synthetase family. Type 2 subfamily.|||Constitutively expressed in a constant ratio along the growth of the bacterium.|||Cytoplasm|||Homodimer. Makes part of a ribonucleoprotein particle (RNP) called transamidosome that allows channelling of the aa-tRNA from non-discriminating aspartyl-tRNA synthetase active site to the GatCAB amidotransferase site. The transamidosome complex is formed by two GatCABs, one dimeric ND-AspRSs and two tRNAs(Asn) molecules. http://togogenome.org/gene/300852:TTH_RS03955 ^@ http://purl.uniprot.org/uniprot/Q5SK86 ^@ Similarity ^@ Belongs to the short-chain dehydrogenases/reductases (SDR) family. http://togogenome.org/gene/300852:TTH_RS06390 ^@ http://purl.uniprot.org/uniprot/Q5SIV4 ^@ Cofactor|||Domain|||Function|||Similarity|||Subunit ^@ Belongs to the DnaG primase family.|||Binds 1 zinc ion per monomer.|||Contains an N-terminal zinc-binding domain, a central core domain that contains the primase activity, and a C-terminal DnaB-binding domain.|||Monomer. Interacts with DnaB.|||RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication. http://togogenome.org/gene/300852:TTH_RS01010 ^@ http://purl.uniprot.org/uniprot/Q5SLV0 ^@ Subcellular Location Annotation ^@ Membrane http://togogenome.org/gene/300852:TTH_RS01100 ^@ http://purl.uniprot.org/uniprot/Q5SLT2 ^@ Subcellular Location Annotation ^@ Membrane http://togogenome.org/gene/300852:TTH_RS02435 ^@ http://purl.uniprot.org/uniprot/Q5SL24 ^@ Similarity ^@ Belongs to the bacterial solute-binding protein 5 family. http://togogenome.org/gene/300852:TTH_RS09805 ^@ http://purl.uniprot.org/uniprot/Q5SH00 ^@ Subcellular Location Annotation ^@ Membrane http://togogenome.org/gene/300852:TTH_RS00740 ^@ http://purl.uniprot.org/uniprot/Q5SM03 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the EPSP synthase family. MurA subfamily.|||Cell wall formation. Adds enolpyruvyl to UDP-N-acetylglucosamine.|||Cytoplasm http://togogenome.org/gene/300852:TTH_RS08245 ^@ http://purl.uniprot.org/uniprot/Q5SHV1 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the binding-protein-dependent transport system permease family.|||Cell membrane http://togogenome.org/gene/300852:TTH_RS06600 ^@ http://purl.uniprot.org/uniprot/Q5SIR1 ^@ Subcellular Location Annotation ^@ Membrane http://togogenome.org/gene/300852:TTH_RS09175 ^@ http://purl.uniprot.org/uniprot/Q5SHC3 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the binding-protein-dependent transport system permease family.|||Cell membrane http://togogenome.org/gene/300852:TTH_RS04680 ^@ http://purl.uniprot.org/uniprot/Q5SJV0 ^@ Similarity ^@ Belongs to the 4-hydroxybenzoyl-CoA thioesterase family. http://togogenome.org/gene/300852:TTH_RS02175 ^@ http://purl.uniprot.org/uniprot/Q5SL76 ^@ Domain|||Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the thiolase-like superfamily. FabH family.|||Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Catalyzes the first condensation reaction which initiates fatty acid synthesis and may therefore play a role in governing the total rate of fatty acid production. Possesses both acetoacetyl-ACP synthase and acetyl transacylase activities. Its substrate specificity determines the biosynthesis of branched-chain and/or straight-chain of fatty acids.|||Cytoplasm|||Homodimer.|||The last Arg residue of the ACP-binding site is essential for the weak association between ACP/AcpP and FabH. http://togogenome.org/gene/300852:TTH_RS02975 ^@ http://purl.uniprot.org/uniprot/Q5SKS2 ^@ Similarity ^@ Belongs to the small heat shock protein (HSP20) family. http://togogenome.org/gene/300852:TTH_RS07330 ^@ http://purl.uniprot.org/uniprot/Q5SIC6 ^@ Similarity ^@ Belongs to the acetyl-CoA hydrolase/transferase family. http://togogenome.org/gene/300852:TTH_RS05950 ^@ http://purl.uniprot.org/uniprot/Q5SJ41 ^@ Subcellular Location Annotation ^@ Membrane http://togogenome.org/gene/300852:TTH_RS07175 ^@ http://purl.uniprot.org/uniprot/Q5SIF2 ^@ Similarity ^@ Belongs to the 5'-nucleotidase family. http://togogenome.org/gene/300852:TTH_RS07370 ^@ http://purl.uniprot.org/uniprot/Q5SIB8 ^@ Similarity ^@ Belongs to the cytochrome b560 family. http://togogenome.org/gene/300852:TTH_RS01150 ^@ http://purl.uniprot.org/uniprot/Q5SLS2 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the vitamin uptake transporter (VUT/ECF) (TC 2.A.88) family. Q precursor transporter subfamily.|||Cell inner membrane|||Involved in the import of queuosine (Q) precursors, required for Q precursor salvage. http://togogenome.org/gene/300852:TTH_RS03945 ^@ http://purl.uniprot.org/uniprot/Q5SK88 ^@ Activity Regulation|||Function|||Similarity|||Subunit ^@ Belongs to the trans-sulfuration enzymes family.|||Catalyzes the conversion of O-acetyl-L-homoserine (OAH) into homocysteine in the methionine biosynthesis pathway. Has weak activity with O-acetyl-L-serine, O-phospho-L-serine, L-serine, O-succinyl-L-homoserine and L-homoserine. Shows low CTT beta-lyase activity and very low CTT gamma-synthase activity.|||Homotetramer.|||Inhibited by the carbonyl reagents hydroxylamine and phenylhydrazine. Also inhibited by methionine and propargylglycine. http://togogenome.org/gene/300852:TTH_RS05935 ^@ http://purl.uniprot.org/uniprot/Q5SJ44 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the binding-protein-dependent transport system permease family.|||Cell membrane|||Membrane http://togogenome.org/gene/300852:TTH_RS08130 ^@ http://purl.uniprot.org/uniprot/Q5SHX4 ^@ Similarity ^@ Belongs to the GTP cyclohydrolase I type 2/NIF3 family. http://togogenome.org/gene/300852:TTH_RS09590 ^@ http://purl.uniprot.org/uniprot/Q5SH39 ^@ Similarity ^@ Belongs to the 2-oxoacid dehydrogenase family. http://togogenome.org/gene/300852:TTH_RS06700 ^@ http://purl.uniprot.org/uniprot/Q9RA54 ^@ Function|||Similarity ^@ Belongs to the DNA mismatch repair MutL/HexB family.|||This protein is involved in the repair of mismatches in DNA. It is required for dam-dependent methyl-directed DNA mismatch repair. May act as a 'molecular matchmaker', a protein that promotes the formation of a stable complex between two or more DNA-binding proteins in an ATP-dependent manner without itself being part of a final effector complex. http://togogenome.org/gene/300852:TTH_RS10735 ^@ http://purl.uniprot.org/uniprot/Q53W21 ^@ Function|||Miscellaneous|||Similarity|||Subunit ^@ Belongs to the CRISPR-associated endonuclease Cas1 family.|||CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Acts as a dsDNA endonuclease. Involved in the integration of spacer DNA into the CRISPR cassette.|||Homodimer, forms a heterotetramer with a Cas2 homodimer.|||Probably encoded in a type III-A CRISPR locus. http://togogenome.org/gene/300852:TTH_RS05835 ^@ http://purl.uniprot.org/uniprot/Q5SJ65 ^@ Function|||Similarity ^@ Belongs to the uracil-DNA glycosylase (UDG) superfamily. Type 5 (UDGb) family.|||DNA glycosylase with broad substrate specificity. Can remove uracil from double-stranded DNA containing either a U/G, U/A, U/C or U/T base pair (PubMed:12000829, PubMed:17870091, PubMed:24838246). Can also excise hypoxanthine from double-stranded DNA containing G/I, T/I, and A/I base pairs, xanthine from both double-stranded and single stranded DNA, thymine from G/T mismatched DNA, 5'-hydroxymethyluracil and 5'-fluorouracil (PubMed:17870091, PubMed:24838246). http://togogenome.org/gene/300852:TTH_RS07310 ^@ http://purl.uniprot.org/uniprot/Q7SIB9 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the HisA/HisF family.|||Cytoplasm|||Heterodimer of HisH and HisF.|||IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the HisH subunit. http://togogenome.org/gene/300852:TTH_RS08700 ^@ http://purl.uniprot.org/uniprot/Q5SHL3 ^@ Function|||Similarity ^@ Belongs to the relA/spoT family.|||In eubacteria ppGpp (guanosine 3'-diphosphate 5'-diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. http://togogenome.org/gene/300852:TTH_RS02865 ^@ http://purl.uniprot.org/uniprot/Q5SKU4 ^@ Similarity ^@ Belongs to the class-I aminoacyl-tRNA synthetase family. http://togogenome.org/gene/300852:TTH_RS02165 ^@ http://purl.uniprot.org/uniprot/Q5SL78 ^@ Function|||Similarity|||Subunit ^@ Belongs to the short-chain dehydrogenases/reductases (SDR) family.|||Catalyzes the NADPH-dependent reduction of beta-ketoacyl-ACP substrates to beta-hydroxyacyl-ACP products, the first reductive step in the elongation cycle of fatty acid biosynthesis.|||Homotetramer. http://togogenome.org/gene/300852:TTH_RS07515 ^@ http://purl.uniprot.org/uniprot/Q5SI90 ^@ Similarity ^@ Belongs to the small heat shock protein (HSP20) family. http://togogenome.org/gene/300852:TTH_RS02595 ^@ http://purl.uniprot.org/uniprot/Q5SKZ6 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the anaerobic coproporphyrinogen-III oxidase family. HemW subfamily.|||Cytoplasm|||Probably acts as a heme chaperone, transferring heme to an unknown acceptor. Binds one molecule of heme per monomer, possibly covalently. Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine. http://togogenome.org/gene/300852:TTH_RS02360 ^@ http://purl.uniprot.org/uniprot/Q5SL39 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the ABC-3 integral membrane protein family.|||Cell membrane|||Membrane http://togogenome.org/gene/300852:TTH_RS06075 ^@ http://purl.uniprot.org/uniprot/Q5SJ18 ^@ Function|||Miscellaneous|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the ArgJ family.|||Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of N-acetylglutamate from glutamate and acetyl-CoA as the acetyl donor, and of ornithine by transacetylation between N(2)-acetylornithine and glutamate.|||Cytoplasm|||Heterotetramer of two alpha and two beta chains.|||Some bacteria possess a monofunctional ArgJ, i.e. capable of catalyzing only the fifth step of the arginine biosynthetic pathway. http://togogenome.org/gene/300852:TTH_RS02785 ^@ http://purl.uniprot.org/uniprot/Q5SKV9 ^@ Similarity ^@ Belongs to the aspartokinase family. http://togogenome.org/gene/300852:TTH_RS02805 ^@ http://purl.uniprot.org/uniprot/Q5SKV5 ^@ Caution|||Cofactor|||Function|||Similarity|||Subunit ^@ Belongs to the succinate/malate CoA ligase beta subunit family.|||Binds 1 Mg(2+) ion per subunit.|||Heterotetramer of two alpha and two beta subunits.|||Lacks conserved residue(s) required for the propagation of feature annotation.|||Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit. http://togogenome.org/gene/300852:TTH_RS06490 ^@ http://purl.uniprot.org/uniprot/Q5SIT4 ^@ Disruption Phenotype|||Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the methyltransferase superfamily. RlmI family.|||Cytoplasm|||Lack of methylation of cytosine at position 1962 (m5C1962) of 23S rRNA.|||Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA. http://togogenome.org/gene/300852:TTH_RS02120 ^@ http://purl.uniprot.org/uniprot/Q5SL87 ^@ Activity Regulation|||Domain|||Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the RuvB family.|||Cytoplasm|||Has 3 domains, the large (RuvB-L, also called N) and small ATPase (RuvB-S, also called C) domains and the C-terminal head (RuvB-H, also called M) domain (PubMed:11171970). The head domain binds DNA, while the ATPase domains jointly bind ATP, ADP or are empty depending on the state of the subunit in the translocation cycle. During a single DNA translocation step the structure of each domain remains the same, but their relative positions change. Domains M and C are flexible; they change conformations whenn bound to whole RuvA and only domain III of RuvA; domain C probably binds Holliday junction DNA (PubMed:12408833).|||Homohexamer (Probable). Forms a complex with RuvA (PubMed:11171970, PubMed:12408833, PubMed:17981150) (Probable). Electron microscopic images suggest 2 closely interacting RuvA tetramers sandwich the HJ DNA; each tetramer associates with an RuvB hexamer (PubMed:12408833, PubMed:17981150, PubMed:18068124). Forms 2 complexes with Holliday junction (HJ) DNA which probably have 1 and 2 RuvA tetramers per complex (called complex I and complex II) (PubMed:11245216).Forms an RuvA(8)-RuvB(12)-Holliday junction (HJ) complex. HJ DNA is sandwiched between 2 RuvA tetramers; dsDNA enters through RuvA and exits via RuvB. An RuvB hexamer assembles on each DNA strand where it exits the tetramer. Each RuvB hexamer is contacted by two RuvA subunits (via domain III) on 2 adjacent RuvB subunits; this complex drives branch migration. In the full resolvosome a probable DNA-RuvA(4)-RuvB(12)-RuvC(2) complex forms which resolves the HJ.|||RuvB is a Mg(2+)-dependent, DNA-dependent ATPase with an equal preference for supercoiled and linear dsDNA; all (d)NTPs tested were efficiently hydrolyzed. Promotes Holliday junction (HJ) dissociation at 60 degrees Celsius in the presence of ATP but not ATP-gamma-S or ADP; (d)ATP, (d)CTP and dTTP also power dissociation in the absence of any RuvA (PubMed:10485292, PubMed:18068124). RuvA stimulates the ATPase of RuvB in the presence of dsDNA and HJ branch migration by RuvB (PubMed:11245216, PubMed:18068124). Excess RuvB stimulates some branch migration in vitro even in the presence of mutant RuvA (PubMed:18068124).|||The RuvA-RuvB-RuvC complex processes Holliday junction (HJ) DNA during genetic recombination and DNA repair, while the RuvA-RuvB complex plays an important role in the rescue of blocked DNA replication forks via replication fork reversal (RFR). RuvA specifically binds to HJ cruciform DNA, conferring on it an open structure. The RuvB hexamer acts as an ATP-dependent pump, pulling dsDNA into and through the RuvAB complex. RuvB forms 2 homohexamers on either side of HJ DNA bound by 1 or 2 RuvA tetramers; 4 subunits per hexamer contact DNA at a time. Coordinated motions by a converter formed by DNA-disengaged RuvB subunits stimulates ATP hydrolysis and nucleotide exchange. Immobilization of the converter enables RuvB to convert the ATP-contained energy into a lever motion, pulling 2 nucleotides of DNA out of the RuvA tetramer per ATP hydrolyzed, thus driving DNA branch migration. The RuvB motors rotate together with the DNA substrate, which together with the progressing nucleotide cycle form the mechanistic basis for DNA recombination by continuous HJ branch migration. Branch migration allows RuvC to scan DNA until it finds its consensus sequence, where it cleaves and resolves cruciform DNA.|||The activity of RuvB is enhanced by E.coli RuvA. http://togogenome.org/gene/300852:TTH_RS08430 ^@ http://purl.uniprot.org/uniprot/P80376 ^@ Function|||Similarity|||Subunit ^@ Belongs to the universal ribosomal protein uS11 family.|||Located on the upper part of the platform of the 30S subunit, where it bridges several disparate RNA helices of the 16S rRNA. Forms part of the Shine-Dalgarno cleft in the 70S ribosome, where it interacts both with the Shine-Dalgarno helix and mRNA.|||Part of the 30S ribosomal subunit. Interacts with proteins S7 and S18. Binds to the C-terminus of IF-3; however exactly how IF-3 interacts with the 30S subunit is unclear. May contact the C-terminus of Era. http://togogenome.org/gene/300852:TTH_RS04485 ^@ http://purl.uniprot.org/uniprot/Q5SJY4 ^@ Function|||Subcellular Location Annotation ^@ Catalyzes the Claisen rearrangement of chorismate to prephenate. Probably involved in the aromatic amino acid biosynthesis.|||Cytoplasm http://togogenome.org/gene/300852:TTH_RS10890 ^@ http://purl.uniprot.org/uniprot/Q53VZ3 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the binding-protein-dependent transport system permease family.|||Cell membrane http://togogenome.org/gene/300852:TTH_RS05780 ^@ http://purl.uniprot.org/uniprot/Q5SJ76 ^@ Function|||Similarity|||Subunit ^@ Belongs to the universal ribosomal protein uS15 family.|||Forms an intersubunit bridge (bridge B4) with the 23S rRNA of the 50S subunit in the ribosome.|||One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it helps nucleate assembly of the platform of the 30S subunit by binding and bridging several RNA helices of the 16S rRNA.|||Part of the 30S ribosomal subunit. Forms a bridge to the 50S subunit in the 70S ribosome, contacting the 23S rRNA. http://togogenome.org/gene/300852:TTH_RS10755 ^@ http://purl.uniprot.org/uniprot/Q53W19 ^@ Domain|||Function|||Miscellaneous|||Similarity|||Subunit ^@ Belongs to the CRISPR-associated Cas10/Csm1 family.|||CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (Probable). The type III-A Csm effector complex binds crRNA and acts as a crRNA-guided RNase, DNase and cyclic oligoadenylate synthase; binding of target RNA cognate to the crRNA is required for all activities (Probable).|||Encoded in a type III-A CRISPR locus.|||Probably part of the Csm effector complex, that includes Cas10, Csm2, Csm3, Csm4, Csm5 and mature crRNA.|||The N-terminal HD domain has ssDNase activity. The C-terminal GGDEF domain has the cOA synthesis activity.|||This subunit is a single-strand-specific deoxyribonuclease (ssDNase) which digests both linear and circular ssDNA; it has both exo- and endonuclease activity.|||When associated with the ternary Csm effector complex (the crRNA, Cas proteins and a cognate target ssRNA) synthesizes cyclic oligoadenylates (cOA) from ATP. The active cOA in this bacteria is cyclic tetraadenylate (cA4), presumably made by this enzyme. cOAs are second messengers that stimulate the ssRNase activity of Csm6, inducing an antiviral state important for defense against invading nucleic acids.|||ssDNase activity is stimulated in the ternary Csm effector complex; binding of cognate target RNA activates the ssDNase, as the target RNA is degraded ssDNA activity decreases. http://togogenome.org/gene/300852:TTH_RS05660 ^@ http://purl.uniprot.org/uniprot/Q5SJA1 ^@ Activity Regulation|||Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Allosterically activated by fructose 1,6-bisphosphate (FBP).|||Belongs to the LDH/MDH superfamily. LDH family.|||Catalyzes the conversion of lactate to pyruvate.|||Cytoplasm|||Homotetramer. http://togogenome.org/gene/300852:TTH_RS00005 ^@ http://purl.uniprot.org/uniprot/Q5SME2 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the beta sliding clamp family.|||Confers DNA tethering and processivity to DNA polymerases and other proteins. Acts as a clamp, forming a ring around DNA (a reaction catalyzed by the clamp-loading complex) which diffuses in an ATP-independent manner freely and bidirectionally along dsDNA. Initially characterized for its ability to contact the catalytic subunit of DNA polymerase III (Pol III), a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria; Pol III exhibits 3'-5' exonuclease proofreading activity. The beta chain is required for initiation of replication as well as for processivity of DNA replication.|||Cytoplasm|||Forms a ring-shaped head-to-tail homodimer around DNA. http://togogenome.org/gene/300852:TTH_RS07555 ^@ http://purl.uniprot.org/uniprot/Q5SI82 ^@ Activity Regulation|||Cofactor|||Domain|||Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins. Plays a role in the quality control of integral membrane proteins.|||Binds 1 zinc ion per subunit.|||Cell inner membrane|||Degrades preferentially unfolded substrates in a processive, ATP-dependent manner, usually after hydrophobic residues.|||In the C-terminal section; belongs to the peptidase M41 family.|||In the central section; belongs to the AAA ATPase family.|||The isolated soluble domain (residues 126-624) forms a stable hexamer in which the AAA+ domains (residues 126-400) are alternatively open or closed.|||The open AAA+ domain (residues 126-400) probably allows nucleotide exchange and has the protease active site, while the closed domain is the active ATPase domain but the protease is inactive.|||The proteolytic activity is dependent on ATP, both the ATPase and protease activities are inhibited by ADP. http://togogenome.org/gene/300852:TTH_RS08420 ^@ http://purl.uniprot.org/uniprot/Q5SHR6 ^@ Domain|||Function|||Similarity|||Subunit ^@ Belongs to the RNA polymerase alpha chain family.|||DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.|||Homodimer. The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription (By similarity).|||The N-terminal domain is essential for RNAP assembly and basal transcription, whereas the C-terminal domain is involved in interaction with transcriptional regulators and with upstream promoter elements. http://togogenome.org/gene/300852:TTH_RS00645 ^@ http://purl.uniprot.org/uniprot/Q5SM21 ^@ Similarity ^@ Belongs to the manganese catalase family. http://togogenome.org/gene/300852:TTH_RS06560 ^@ http://purl.uniprot.org/uniprot/Q5SIR9 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the ABC transporter superfamily.|||Cell membrane|||Homodimer. Forms a membrane-associated complex with FtsX.|||Part of the ABC transporter FtsEX involved in cellular division. http://togogenome.org/gene/300852:TTH_RS09205 ^@ http://purl.uniprot.org/uniprot/Q8RQE9 ^@ Function|||Similarity|||Subunit ^@ Belongs to the RNA polymerase beta chain family.|||DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.|||The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription. http://togogenome.org/gene/300852:TTH_RS10415 ^@ http://purl.uniprot.org/uniprot/Q53W77 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the binding-protein-dependent transport system permease family.|||Cell membrane http://togogenome.org/gene/300852:TTH_RS02020 ^@ http://purl.uniprot.org/uniprot/Q5SLA8 ^@ Cofactor|||Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Binds 1 Mg(2+) ion per subunit.|||Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc). The C-terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N-acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5-triphosphate), a reaction catalyzed by the N-terminal domain.|||Cytoplasm|||Homotrimer.|||In the C-terminal section; belongs to the transferase hexapeptide repeat family.|||In the N-terminal section; belongs to the N-acetylglucosamine-1-phosphate uridyltransferase family. http://togogenome.org/gene/300852:TTH_RS02075 ^@ http://purl.uniprot.org/uniprot/Q5SL96 ^@ Subcellular Location Annotation ^@ Membrane http://togogenome.org/gene/300852:TTH_RS05315 ^@ http://purl.uniprot.org/uniprot/Q5SJG4 ^@ Cofactor|||Similarity|||Subcellular Location Annotation ^@ Belongs to the bacterial diacylglycerol kinase family.|||Membrane|||Mn(2+), Zn(2+), Cd(2+) and Co(2+) support activity to lesser extents. http://togogenome.org/gene/300852:TTH_RS06330 ^@ http://purl.uniprot.org/uniprot/Q5SIW6 ^@ Function|||PTM|||Similarity ^@ Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme.|||Belongs to the ATP-dependent AMP-binding enzyme family.|||Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA. http://togogenome.org/gene/300852:TTH_RS00720 ^@ http://purl.uniprot.org/uniprot/Q5SM07 ^@ Similarity ^@ Belongs to the SIMIBI class G3E GTPase family. ArgK/MeaB subfamily. http://togogenome.org/gene/300852:TTH_RS01025 ^@ http://purl.uniprot.org/uniprot/Q5SLU7 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the bacterial solute-binding protein 7 family.|||Homodimer.|||Part of the tripartite ATP-independent periplasmic (TRAP) transport system.|||Periplasm http://togogenome.org/gene/300852:TTH_RS04110 ^@ http://purl.uniprot.org/uniprot/Q5SK58 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the GSP F family.|||Cell inner membrane|||Essential inner membrane component of the type IV pilus (T4P) that plays a role in surface and host cell adhesion, colonization, biofilm maturation, virulence, and twitching, a form of surface-associated motility facilitated by cycles of extension, adhesion, and retraction of T4P fibers. Controls both pilus assembly and disassembly and plays an important role in PilB localization to the complex and ATPase activity.|||Homotetramer (PubMed:20455262). Interacts with PilB (By similarity). http://togogenome.org/gene/300852:TTH_RS00225 ^@ http://purl.uniprot.org/uniprot/P60494 ^@ Similarity|||Subunit ^@ Belongs to the bacterial ribosomal protein bL28 family.|||Part of the 50S ribosomal subunit. http://togogenome.org/gene/300852:TTH_RS01520 ^@ http://purl.uniprot.org/uniprot/Q5SLK5 ^@ Cofactor|||Function|||Similarity ^@ Belongs to the 2-oxoacid dehydrogenase family.|||Binds 1 lipoyl cofactor covalently.|||E2 component of the 2-oxoglutarate dehydrogenase (OGDH) complex which catalyzes the second step in the conversion of 2-oxoglutarate to succinyl-CoA and CO(2). http://togogenome.org/gene/300852:TTH_RS06545 ^@ http://purl.uniprot.org/uniprot/Q5SIS2 ^@ Cofactor|||Similarity ^@ Belongs to the Fur family.|||Binds 1 Mn(2+) or Fe(2+) ion per subunit.|||Binds 1 zinc ion per subunit. http://togogenome.org/gene/300852:TTH_RS01000 ^@ http://purl.uniprot.org/uniprot/Q5SLV2 ^@ Function|||Similarity ^@ Belongs to the DHPS family.|||Catalyzes the condensation of para-aminobenzoate (pABA) with 6-hydroxymethyl-7,8-dihydropterin diphosphate (DHPt-PP) to form 7,8-dihydropteroate (H2Pte), the immediate precursor of folate derivatives. http://togogenome.org/gene/300852:TTH_RS05300 ^@ http://purl.uniprot.org/uniprot/Q5SJG7 ^@ Cofactor|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the class-II aminoacyl-tRNA synthetase family.|||Binds 3 Mg(2+) ions per subunit.|||Cytoplasm|||Homodimer. http://togogenome.org/gene/300852:TTH_RS11205 ^@ http://purl.uniprot.org/uniprot/Q53VT9 ^@ Similarity ^@ Belongs to the aldehyde dehydrogenase family. http://togogenome.org/gene/300852:TTH_RS00505 ^@ http://purl.uniprot.org/uniprot/Q56229 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the complex I subunit 2 family.|||Cell inner membrane|||NDH-1 is composed of 15 different subunits, Nqo1 to Nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits Nqo7, Nqo8 and Nqo10 to Nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits Nqo1 to Nqo6, Nqo9 and Nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers.|||NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP. http://togogenome.org/gene/300852:TTH_RS10325 ^@ http://purl.uniprot.org/uniprot/Q53W95 ^@ Cofactor|||Similarity ^@ Belongs to the alkaline phosphatase family.|||Binds 1 Mg(2+) ion.|||Binds 2 Zn(2+) ions. http://togogenome.org/gene/300852:TTH_RS00455 ^@ http://purl.uniprot.org/uniprot/Q56220 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the complex I 49 kDa subunit family.|||Cell membrane|||NDH-1 is composed of 15 different subunits, Nqo1 to Nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits Nqo7, Nqo8 and Nqo10 to Nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits Nqo1 to Nqo6, Nqo9 and Nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers. This subunit interacts extensively with Nqo6.|||NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP. The Nqo4 subunit may contain the quinone-binding site. http://togogenome.org/gene/300852:TTH_RS03435 ^@ http://purl.uniprot.org/uniprot/Q5SKI4 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the auxin efflux carrier (TC 2.A.69) family.|||Cell membrane|||Membrane http://togogenome.org/gene/300852:TTH_RS04055 ^@ http://purl.uniprot.org/uniprot/Q5SK66 ^@ Similarity ^@ Belongs to the aspartate/ornithine carbamoyltransferase superfamily. ATCase family. http://togogenome.org/gene/300852:TTH_RS06565 ^@ http://purl.uniprot.org/uniprot/Q5SIR8 ^@ Similarity ^@ Belongs to the peptidase S41A family. http://togogenome.org/gene/300852:TTH_RS03215 ^@ http://purl.uniprot.org/uniprot/Q5SKM6 ^@ Function|||PTM|||Similarity ^@ Belongs to the NAPRTase family.|||Catalyzes the first step in the biosynthesis of NAD from nicotinic acid, the ATP-dependent synthesis of beta-nicotinate D-ribonucleotide from nicotinate and 5-phospho-D-ribose 1-phosphate.|||Transiently phosphorylated on a His residue during the reaction cycle. Phosphorylation strongly increases the affinity for substrates and increases the rate of nicotinate D-ribonucleotide production. Dephosphorylation regenerates the low-affinity form of the enzyme, leading to product release. http://togogenome.org/gene/300852:TTH_RS08085 ^@ http://purl.uniprot.org/uniprot/Q5SHY3 ^@ Subcellular Location Annotation ^@ Membrane http://togogenome.org/gene/300852:TTH_RS06520 ^@ http://purl.uniprot.org/uniprot/Q5SIS8 ^@ Subcellular Location Annotation ^@ Membrane http://togogenome.org/gene/300852:TTH_RS00460 ^@ http://purl.uniprot.org/uniprot/Q56221 ^@ Cofactor|||Domain|||Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the complex I 24 kDa subunit family.|||Binds 1 [2Fe-2S] cluster. This [2Fe-2S] cluster is referred to as N1a.|||Cell membrane|||NDH-1 is composed of 15 different subunits, Nqo1 to Nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits Nqo7, Nqo8 and Nqo10 to Nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits Nqo1 to Nqo6, Nqo9 and Nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers.|||NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP.|||The subunit can be divided into two domains: an N-terminal four-helical bundle (residues 2 to 74) involved in interactions with subunits Nqo1 and Nqo3, and a thioredoxin-like C-terminal domain (residues 75 to 180) that coordinates the binuclear cluster N1a. http://togogenome.org/gene/300852:TTH_RS02730 ^@ http://purl.uniprot.org/uniprot/Q5SKW9 ^@ Cofactor|||Function|||Similarity|||Subunit ^@ Belongs to the GcvH family.|||Binds 1 lipoyl cofactor covalently.|||The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein.|||The glycine cleavage system is composed of four proteins: P, T, L and H (By similarity). Monomer. http://togogenome.org/gene/300852:TTH_RS02905 ^@ http://purl.uniprot.org/uniprot/P61503 ^@ Cofactor|||Function|||Similarity|||Subunit ^@ Belongs to the iron/manganese superoxide dismutase family.|||Binds 1 Mn(2+) ion per subunit.|||Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.|||Homotetramer. http://togogenome.org/gene/300852:TTH_RS07115 ^@ http://purl.uniprot.org/uniprot/Q5SIG4 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the CcmE/CycJ family.|||Cell inner membrane|||Heme chaperone required for the biogenesis of c-type cytochromes. Transiently binds heme delivered by CcmC and transfers the heme to apo-cytochromes in a process facilitated by CcmF and CcmH. http://togogenome.org/gene/300852:TTH_RS06025 ^@ http://purl.uniprot.org/uniprot/Q5SJ28 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the DeoC/FbaB aldolase family. DeoC type 1 subfamily.|||Catalyzes a reversible aldol reaction between acetaldehyde and D-glyceraldehyde 3-phosphate to generate 2-deoxy-D-ribose 5-phosphate.|||Cytoplasm|||Homotetramer, in solution and in the crystal structure. http://togogenome.org/gene/300852:TTH_RS01080 ^@ http://purl.uniprot.org/uniprot/Q5SLT6 ^@ Function ^@ The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane. http://togogenome.org/gene/300852:TTH_RS10760 ^@ http://purl.uniprot.org/uniprot/Q53WF6 ^@ Function|||Similarity ^@ Belongs to the CRISPR-associated Csm2 family.|||This subunit may be involved in monitoring complementarity of crRNA and target RNA. http://togogenome.org/gene/300852:TTH_RS09505 ^@ http://purl.uniprot.org/uniprot/P56881 ^@ Cofactor|||Domain|||Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the class-II aminoacyl-tRNA synthetase family.|||Binds 1 zinc ion per subunit.|||Catalyzes the attachment of threonine to tRNA(Thr) in a two-step reaction: L-threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr). Also edits incorrectly charged L-seryl-tRNA(Thr).|||Cytoplasm|||Homodimer.|||The C-terminal domain recognizes the anticodon bases. http://togogenome.org/gene/300852:TTH_RS03565 ^@ http://purl.uniprot.org/uniprot/Q5SKF9 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the exbB/tolQ family.|||Membrane http://togogenome.org/gene/300852:TTH_RS04420 ^@ http://purl.uniprot.org/uniprot/Q5SJZ6 ^@ Function|||Similarity ^@ Belongs to the DXR family.|||Catalyzes the NADPH-dependent rearrangement and reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D-erythritol 4-phosphate (MEP). http://togogenome.org/gene/300852:TTH_RS02160 ^@ http://purl.uniprot.org/uniprot/Q5SL79 ^@ Function|||PTM|||Similarity|||Subcellular Location Annotation ^@ 4'-phosphopantetheine is transferred from CoA to a specific serine of apo-ACP by AcpS. This modification is essential for activity because fatty acids are bound in thioester linkage to the sulfhydryl of the prosthetic group.|||Belongs to the acyl carrier protein (ACP) family.|||Carrier of the growing fatty acid chain in fatty acid biosynthesis.|||Cytoplasm http://togogenome.org/gene/300852:TTH_RS01190 ^@ http://purl.uniprot.org/uniprot/Q5SLR4 ^@ Function|||Similarity|||Subunit ^@ Belongs to the BCKDHA family.|||Heterotetramer of two alpha and two beta chains. Directly associated with ODBB in the E1 complex.|||The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3) (By similarity). http://togogenome.org/gene/300852:TTH_RS05460 ^@ http://purl.uniprot.org/uniprot/Q5SJE1 ^@ Cofactor|||Function|||Similarity|||Subunit ^@ Belongs to the bacterial ribosomal protein bL31 family. Type A subfamily.|||Binds 1 zinc ion per subunit.|||Binds the 23S rRNA.|||Part of the 50S ribosomal subunit. http://togogenome.org/gene/300852:TTH_RS06455 ^@ http://purl.uniprot.org/uniprot/Q56403 ^@ Function|||Similarity ^@ Belongs to the ATPase alpha/beta chains family.|||Produces ATP from ADP in the presence of a proton gradient across the membrane. The V-type alpha chain is a catalytic subunit. http://togogenome.org/gene/300852:TTH_RS04760 ^@ http://purl.uniprot.org/uniprot/Q5SJS3 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the drug/metabolite transporter (DMT) superfamily. Small multidrug resistance (SMR) (TC 2.A.7.1) family.|||Cell membrane|||Membrane http://togogenome.org/gene/300852:TTH_RS04685 ^@ http://purl.uniprot.org/uniprot/Q5SJU9 ^@ Caution|||Cofactor|||Miscellaneous|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. ArgD subfamily.|||Binds 1 pyridoxal phosphate per subunit.|||Cytoplasm|||Homodimer.|||Lacks conserved residue(s) required for the propagation of feature annotation.|||May also have succinyldiaminopimelate aminotransferase activity, thus carrying out the corresponding step in lysine biosynthesis. http://togogenome.org/gene/300852:TTH_RS01140 ^@ http://purl.uniprot.org/uniprot/Q5SLS4 ^@ Similarity ^@ Belongs to the pseudouridine synthase RsuA family. http://togogenome.org/gene/300852:TTH_RS09840 ^@ http://purl.uniprot.org/uniprot/Q5SGZ3 ^@ Cofactor|||Function|||Miscellaneous|||Similarity|||Subcellular Location Annotation ^@ Belongs to the radical SAM superfamily. RlmN family.|||Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.|||Cytoplasm|||Reaction proceeds by a ping-pong mechanism involving intermediate methylation of a conserved cysteine residue.|||Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs. http://togogenome.org/gene/300852:TTH_RS09945 ^@ http://purl.uniprot.org/uniprot/Q5SGX2 ^@ Cofactor|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 1 subfamily.|||Binds 2 magnesium ions per tetramer.|||Cytoplasm|||Tetramer of two alpha and two beta subunits. http://togogenome.org/gene/300852:TTH_RS10780 ^@ http://purl.uniprot.org/uniprot/Q53W17 ^@ Activity Regulation|||Cofactor|||Domain|||Function|||Miscellaneous|||Similarity|||Subunit ^@ A single-strand-specific endoribonuclease (ssRNase) producing free 5'-OH (PubMed:26763118). Activity is approximately 1000-fold stimulated by cyclic oligoadenylate (cOA); only cyclic tetraadenylate (cA4) stimulates the ssRNase activity while linear oligoadenylates do not activate the RNase (PubMed:28663439). Another study showed stimulation by linear tetraadenylate at very high concentrations, but did not examine stimulation by cA4 (PubMed:28722012).|||Belongs to the CRISPR-associated Csm6 family.|||CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (Probable). The type III-A Csm effector complex binds crRNA and acts as a crRNA-guided RNase, DNase and cyclic oligoadenylate synthase; binding of target RNA cognate to the crRNA is required for all activities. This protein is not part of the Csm effector complex (Probable).|||Does not require a metal cofactor.|||Homodimer. The protein forms a twisted, head-to-head dimer; the composite ssRNase active site is formed at the dimer interface.|||Non-specific ssRNase activity is allosterically activated about 1000-fold by cyclic tetraadenylate (cA4), which probably binds to its CARF domain.|||Probably encoded in a type III-A CRISPR locus.|||The N-terminal CRISPR-associated Rossman fold (CARF) probably binds the cA4 effector. ssRNase activity resides in the C-terminal HEPN domain. http://togogenome.org/gene/300852:TTH_RS10010 ^@ http://purl.uniprot.org/uniprot/Q5SGV8 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the MnmG family.|||Cytoplasm|||Homodimer. Heterotetramer of two MnmE and two MnmG subunits.|||NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34. http://togogenome.org/gene/300852:TTH_RS03675 ^@ http://purl.uniprot.org/uniprot/Q5SKD7 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.|||Cell membrane http://togogenome.org/gene/300852:TTH_RS01230 ^@ http://purl.uniprot.org/uniprot/Q5SLQ8 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the AzlC family.|||Membrane http://togogenome.org/gene/300852:TTH_RS00440 ^@ http://purl.uniprot.org/uniprot/Q56217 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the complex I subunit 3 family.|||Cell inner membrane|||NDH-1 is composed of 15 different subunits, Nqo1 to Nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits Nqo7, Nqo8 and Nqo10 to Nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits Nqo1 to Nqo6, Nqo9 and Nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers.|||NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP. http://togogenome.org/gene/300852:TTH_RS07855 ^@ http://purl.uniprot.org/uniprot/Q5SI29 ^@ Cofactor|||Function|||Similarity|||Subunit ^@ Belongs to the LuxS family.|||Binds 1 Fe cation per subunit.|||Homodimer.|||Involved in the synthesis of autoinducer 2 (AI-2) which is secreted by bacteria and is used to communicate both the cell density and the metabolic potential of the environment. The regulation of gene expression in response to changes in cell density is called quorum sensing. Catalyzes the transformation of S-ribosylhomocysteine (RHC) to homocysteine (HC) and 4,5-dihydroxy-2,3-pentadione (DPD). http://togogenome.org/gene/300852:TTH_RS01185 ^@ http://purl.uniprot.org/uniprot/Q5SLR5 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the RNA methyltransferase RlmH family.|||Cytoplasm|||Homodimer.|||Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA. http://togogenome.org/gene/300852:TTH_RS03850 ^@ http://purl.uniprot.org/uniprot/Q5SKA7 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. LepA subfamily.|||Cell inner membrane|||Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner. http://togogenome.org/gene/300852:TTH_RS02540 ^@ http://purl.uniprot.org/uniprot/Q5SL05 ^@ Function|||Similarity ^@ Antitoxin component of a type II toxin-antitoxin (TA) system.|||Belongs to the phD/YefM antitoxin family. http://togogenome.org/gene/300852:TTH_RS08285 ^@ http://purl.uniprot.org/uniprot/Q5SHU3 ^@ Caution|||Cofactor|||Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the ribose-phosphate pyrophosphokinase family. Class I subfamily.|||Binds 2 Mg(2+) ions per subunit.|||Cytoplasm|||Homohexamer.|||Involved in the biosynthesis of the central metabolite phospho-alpha-D-ribosyl-1-pyrophosphate (PRPP) via the transfer of pyrophosphoryl group from ATP to 1-hydroxyl of ribose-5-phosphate (Rib-5-P).|||Lacks conserved residue(s) required for the propagation of feature annotation. http://togogenome.org/gene/300852:TTH_RS04780 ^@ http://purl.uniprot.org/uniprot/Q5SJR9 ^@ Function|||Subunit ^@ Heterodimer of an alpha and a beta chain.|||The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). http://togogenome.org/gene/300852:TTH_RS05760 ^@ http://purl.uniprot.org/uniprot/Q5SJ80 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the cytochrome c oxidase subunit 2 family.|||Cell membrane|||Subunits I and II form the functional core of the enzyme complex. Electrons originating in cytochrome c are transferred via heme a and Cu(A) to the binuclear center formed by heme a3 and Cu(B). http://togogenome.org/gene/300852:TTH_RS04200 ^@ http://purl.uniprot.org/uniprot/Q5SK40 ^@ Similarity ^@ Belongs to the GARS family. http://togogenome.org/gene/300852:TTH_RS01910 ^@ http://purl.uniprot.org/uniprot/Q5SLC8 ^@ Similarity ^@ Belongs to the GSP E family. http://togogenome.org/gene/300852:TTH_RS09790 ^@ http://purl.uniprot.org/uniprot/Q5SH03 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the glutamate 5-kinase family.|||Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.|||Cytoplasm http://togogenome.org/gene/300852:TTH_RS07720 ^@ http://purl.uniprot.org/uniprot/Q5SI55 ^@ Function|||Similarity ^@ Belongs to the RecN family.|||May be involved in recombinational repair of damaged DNA. http://togogenome.org/gene/300852:TTH_RS06555 ^@ http://purl.uniprot.org/uniprot/Q5SIS0 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the HPF/YfiA ribosome-associated protein family. Long HPF subfamily.|||Cytoplasm|||Interacts with 100S ribosomes.|||Required for dimerization of active 70S ribosomes into 100S ribosomes in stationary phase; 100S ribosomes are translationally inactive and sometimes present during exponential growth. http://togogenome.org/gene/300852:TTH_RS06080 ^@ http://purl.uniprot.org/uniprot/Q5SJ17 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the NAGSA dehydrogenase family. Type 1 subfamily.|||Catalyzes the NADPH-dependent reduction of N-acetyl-5-glutamyl phosphate to yield N-acetyl-L-glutamate 5-semialdehyde.|||Cytoplasm http://togogenome.org/gene/300852:TTH_RS09025 ^@ http://purl.uniprot.org/uniprot/Q5SHF0 ^@ Function|||Similarity ^@ Belongs to the NadD family.|||Catalyzes the reversible adenylation of nicotinate mononucleotide (NaMN) to nicotinic acid adenine dinucleotide (NaAD). http://togogenome.org/gene/300852:TTH_RS02155 ^@ http://purl.uniprot.org/uniprot/Q5SL80 ^@ Function|||Similarity ^@ Belongs to the thiolase-like superfamily. Beta-ketoacyl-ACP synthases family.|||Involved in the type II fatty acid elongation cycle. Catalyzes the elongation of a wide range of acyl-ACP by the addition of two carbons from malonyl-ACP to an acyl acceptor. Can efficiently catalyze the conversion of palmitoleoyl-ACP (cis-hexadec-9-enoyl-ACP) to cis-vaccenoyl-ACP (cis-octadec-11-enoyl-ACP), an essential step in the thermal regulation of fatty acid composition. http://togogenome.org/gene/300852:TTH_RS11240 ^@ http://purl.uniprot.org/uniprot/Q53WI0 ^@ Activity Regulation|||Cofactor|||Function|||Similarity|||Subunit ^@ Appears to be allosterically activated by NADH.|||Belongs to the 4-hydroxy-2-oxovalerate aldolase family.|||Catalyzes the retro-aldol cleavage of both 4-hydroxy-2-oxopentanoate (HOPA) and 4-hydroxy-2-oxohexanoate (HOHA) to pyruvate and acetaldehyde or propanaldehyde, respectively. The aldehydes produced by this reaction are directly channeled to the dehydrogenase TTHB247, ensuring that these toxic aldehydes are sequestered from cellular components. Is involved in the meta-cleavage pathway for the degradation of aromatic compounds. Appears to be stereospecific since it can cleave (4S)-4-hydroxy-2-oxopentanoate but not the (4R) isomer. Is not able to catalyze the aldol addition of 2-oxobutyrate with acetaldehyde; this indicates that the enzyme is specific for pyruvate as the carbonyl donor.|||Divalent metal cation. Has the highest activity with Co(2+) as cofactor, followed by Ni(2+) and Mn(2+). Mg(2+) and Ca(2+) are very poor metal cofactors.|||Homodimer. Can also form a heterotetramer composed of two aldolase (TTHB246) and two dehydrogenase (TTHB247) subunits. Upon complex formation, the aldolase shows a 5-fold increase in substrate affinity, while the dehydrogenase shows a 3-fold decrease; the kcat values of each enzyme are reduced by 2-fold when they are in a complex. http://togogenome.org/gene/300852:TTH_RS01465 ^@ http://purl.uniprot.org/uniprot/Q5SLL5 ^@ Activity Regulation|||Cofactor|||Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Allosterically activated by calcium.|||Belongs to the phosphoenolpyruvate carboxykinase (ATP) family.|||Binds 1 Mn(2+) ion per subunit.|||Cytoplasm|||Dimer of dimers.|||Involved in gluconeogenesis. Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP) through direct phosphoryl transfer between the nucleoside triphosphate and OAA (By similarity). http://togogenome.org/gene/300852:TTH_RS09990 ^@ http://purl.uniprot.org/uniprot/Q5SGW2 ^@ Subcellular Location Annotation ^@ Membrane http://togogenome.org/gene/300852:TTH_RS03525 ^@ http://purl.uniprot.org/uniprot/Q5SKG7 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the ThiG family.|||Catalyzes the rearrangement of 1-deoxy-D-xylulose 5-phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S.|||Cytoplasm|||Homotetramer. Forms heterodimers with either ThiH or ThiS. http://togogenome.org/gene/300852:TTH_RS03300 ^@ http://purl.uniprot.org/uniprot/Q5SKL2 ^@ Similarity ^@ Belongs to the peptidase T1B family. HslV subfamily. http://togogenome.org/gene/300852:TTH_RS02180 ^@ http://purl.uniprot.org/uniprot/P80339 ^@ Function|||Similarity|||Subunit ^@ Belongs to the bacterial ribosomal protein bL32 family.|||Found on the solvent side of the large subunit.|||Part of the 50S ribosomal subunit. http://togogenome.org/gene/300852:TTH_RS06475 ^@ http://purl.uniprot.org/uniprot/Q5SIT7 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the V-ATPase proteolipid subunit family.|||Membrane http://togogenome.org/gene/300852:TTH_RS10385 ^@ http://purl.uniprot.org/uniprot/Q53W83 ^@ Function|||Similarity|||Subunit ^@ Belongs to the carbohydrate kinase pfkB family.|||Homohexamer; trimer of dimers.|||Involved in the degradation of glucose via the semi-phosphorylative Entner-Doudoroff pathway. Catalyzes the phosphorylation of 2-keto-3-deoxygluconate (KDG) to produce 2-keto-3-deoxy-6-phosphogluconate (KDPG). http://togogenome.org/gene/300852:TTH_RS02310 ^@ http://purl.uniprot.org/uniprot/Q5SL49 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the UPF0161 family.|||Cell inner membrane|||Could be involved in insertion of integral membrane proteins into the membrane. http://togogenome.org/gene/300852:TTH_RS10765 ^@ http://purl.uniprot.org/uniprot/Q53WF5 ^@ Similarity ^@ Belongs to the CRISPR-associated Csm3 family. http://togogenome.org/gene/300852:TTH_RS05720 ^@ http://purl.uniprot.org/uniprot/Q76G20 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the elongation factor P family.|||Cytoplasm|||Involved in peptide bond synthesis. Stimulates efficient translation and peptide-bond synthesis on native or reconstituted 70S ribosomes in vitro. Probably functions indirectly by altering the affinity of the ribosome for aminoacyl-tRNA, thus increasing their reactivity as acceptors for peptidyl transferase (By similarity). http://togogenome.org/gene/300852:TTH_RS01410 ^@ http://purl.uniprot.org/uniprot/Q5SLM7 ^@ Similarity ^@ Belongs to the peptidase C40 family. http://togogenome.org/gene/300852:TTH_RS01840 ^@ http://purl.uniprot.org/uniprot/Q5SLE3 ^@ Similarity ^@ Belongs to the universal stress protein A family. http://togogenome.org/gene/300852:TTH_RS07600 ^@ http://purl.uniprot.org/uniprot/Q5SI73 ^@ Subcellular Location Annotation ^@ Membrane http://togogenome.org/gene/300852:TTH_RS08440 ^@ http://purl.uniprot.org/uniprot/Q5SHR2 ^@ Cofactor|||Similarity|||Subunit ^@ Belongs to the bacterial ribosomal protein bL36 family.|||Binds 1 zinc ion per subunit.|||Part of the 50S ribosomal subunit. http://togogenome.org/gene/300852:TTH_RS06435 ^@ http://purl.uniprot.org/uniprot/Q5SIU5 ^@ Function|||Similarity ^@ Belongs to the transposase mutator family.|||Required for the transposition of the insertion element. http://togogenome.org/gene/300852:TTH_RS09200 ^@ http://purl.uniprot.org/uniprot/Q8RQE8 ^@ Cofactor|||Function|||Similarity|||Subunit ^@ Belongs to the RNA polymerase beta' chain family.|||Binds 1 Mg(2+) ion per subunit.|||Binds 2 Zn(2+) ions per subunit.|||DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.|||The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription. http://togogenome.org/gene/300852:TTH_RS04270 ^@ http://purl.uniprot.org/uniprot/Q5SK25 ^@ Similarity ^@ Belongs to the Skp family. http://togogenome.org/gene/300852:TTH_RS06505 ^@ http://purl.uniprot.org/uniprot/Q5SIT1 ^@ Similarity ^@ Belongs to the UPF0173 family. http://togogenome.org/gene/300852:TTH_RS01685 ^@ http://purl.uniprot.org/uniprot/Q5SLH4 ^@ Function|||Similarity ^@ Belongs to the RapZ-like family.|||Displays ATPase and GTPase activities. http://togogenome.org/gene/300852:TTH_RS00755 ^@ http://purl.uniprot.org/uniprot/Q5SM00 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the PhoU family.|||Cytoplasm|||Homodimer.|||Plays a role in the regulation of phosphate uptake. http://togogenome.org/gene/300852:TTH_RS04340 ^@ http://purl.uniprot.org/uniprot/Q5SK12 ^@ Similarity ^@ Belongs to the Lgt family. http://togogenome.org/gene/300852:TTH_RS07625 ^@ http://purl.uniprot.org/uniprot/Q5SI68 ^@ Domain|||Function|||Miscellaneous|||Similarity|||Subunit ^@ Belongs to the glutamyl-tRNA reductase family.|||Catalyzes the NADPH-dependent reduction of glutamyl-tRNA(Glu) to glutamate 1-semialdehyde (GSA).|||During catalysis, the active site Cys acts as a nucleophile attacking the alpha-carbonyl group of tRNA-bound glutamate with the formation of a thioester intermediate between enzyme and glutamate, and the concomitant release of tRNA(Glu). The thioester intermediate is finally reduced by direct hydride transfer from NADPH, to form the product GSA.|||Homodimer.|||Possesses an unusual extended V-shaped dimeric structure with each monomer consisting of three distinct domains arranged along a curved 'spinal' alpha-helix. The N-terminal catalytic domain specifically recognizes the glutamate moiety of the substrate. The second domain is the NADPH-binding domain, and the third C-terminal domain is responsible for dimerization. http://togogenome.org/gene/300852:TTH_RS09305 ^@ http://purl.uniprot.org/uniprot/Q5SH97 ^@ Subcellular Location Annotation ^@ Membrane http://togogenome.org/gene/300852:TTH_RS02445 ^@ http://purl.uniprot.org/uniprot/Q5SL22 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the binding-protein-dependent transport system permease family.|||Cell membrane|||Membrane http://togogenome.org/gene/300852:TTH_RS07550 ^@ http://purl.uniprot.org/uniprot/Q56235 ^@ Function|||Induction|||Miscellaneous|||Similarity|||Subunit ^@ Belongs to the heat shock protein 70 family.|||By stress conditions e.g. heat shock (By similarity).|||Cooperates with DnaJ, GrpE and ClpB to reactivate heat-inactivated proteins.|||Forms a heterononamer with DnaJ and DafA in the resting state. Three copies of each protein are present in the complex.|||In the resting state, the DnaK-DnaJ-DafA complex cannot bind substrate. As the substrate becomes sufficiently high, DafA is displaced and an active DnaK-substrate-DnaJ complex is formed. http://togogenome.org/gene/300852:TTH_RS01270 ^@ http://purl.uniprot.org/uniprot/Q5SLQ0 ^@ Function|||Similarity|||Subunit ^@ Belongs to the bacterial ribosomal protein bS18 family.|||Located on the back of the platform of the 30S subunit where it stabilizes the close packing of several RNA helices of the 16S rRNA. Forms part of the Shine-Dalgarno cleft in the 70S ribosome, where it probably interacts with the Shine-Dalgarno helix.|||Part of the 30S ribosomal subunit. Forms a tight heterodimer with protein bS6, also interacts with protein uS11. Binds to the C-terminus of IF3 and to the central section of Era. http://togogenome.org/gene/300852:TTH_RS02955 ^@ http://purl.uniprot.org/uniprot/Q5SKS6 ^@ Similarity ^@ Belongs to the acylphosphatase family. http://togogenome.org/gene/300852:TTH_RS02980 ^@ http://purl.uniprot.org/uniprot/Q5SKS1 ^@ Function|||Similarity|||Subunit ^@ Belongs to the IPP transferase family.|||Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A).|||Monomer. http://togogenome.org/gene/300852:TTH_RS10585 ^@ http://purl.uniprot.org/uniprot/Q53W47 ^@ Subcellular Location Annotation ^@ Cell outer membrane|||Periplasm http://togogenome.org/gene/300852:TTH_RS09600 ^@ http://purl.uniprot.org/uniprot/Q5SH37 ^@ Domain|||Function|||Subcellular Location Annotation|||Subunit ^@ Cell outer membrane|||Exhibits a tripartite organization, with its C-terminal part forming a homotrimeric 28-stranded OM beta-barrel (OMBB), its central part forming a long trimeric coiled coil that can traverse the large periplasmic space, and the extreme N-terminal part forming an SLH domain trimer that can interact with the PG layer.|||Homotrimer.|||Plays an important role in the structural organization and integrity of the cell envelope, bridging the outer membrane to the peptidoglyan layer. Appears to be a nonselective channel. http://togogenome.org/gene/300852:TTH_RS05705 ^@ http://purl.uniprot.org/uniprot/Q5SJ92 ^@ Similarity ^@ Belongs to the asp23 family. http://togogenome.org/gene/300852:TTH_RS02680 ^@ http://purl.uniprot.org/uniprot/Q5SKX9 ^@ Function|||Similarity ^@ Belongs to the AIR carboxylase family. Class I subfamily.|||Catalyzes the conversion of N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) to 4-carboxy-5-aminoimidazole ribonucleotide (CAIR). http://togogenome.org/gene/300852:TTH_RS08045 ^@ http://purl.uniprot.org/uniprot/Q5SHZ1 ^@ Function|||Similarity|||Subunit ^@ Belongs to the bacterial ribosomal protein bL25 family. CTC subfamily.|||Part of the 50S ribosomal subunit. Contacts the 5S rRNA; has been isolated in a complex with 5S rRNA and uL5, 5S rRNA and DNA binding protein II; 5S rRNA and uL18; 5S rRNA, uL5 and DNA binding protein II (Ref.4).|||This is one of 3 proteins that mediate the attachment of the 5S rRNA onto the large ribosomal subunit. http://togogenome.org/gene/300852:TTH_RS03205 ^@ http://purl.uniprot.org/uniprot/Q5SKM8 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the peptidase S14 family.|||Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins.|||Cytoplasm|||Fourteen ClpP subunits assemble into 2 heptameric rings which stack back to back to give a disk-like structure with a central cavity, resembling the structure of eukaryotic proteasomes. http://togogenome.org/gene/300852:TTH_RS05850 ^@ http://purl.uniprot.org/uniprot/P42778 ^@ Cofactor|||Function|||Similarity|||Subunit ^@ Belongs to the peptidase M29 family.|||Binds 2 divalent metal cations per subunit. Can use cobalt, zinc, and possibly also magnesium ions.|||Homodimer.|||Metal-dependent exopeptidase. http://togogenome.org/gene/300852:TTH_RS01210 ^@ http://purl.uniprot.org/uniprot/Q5SLR1 ^@ Similarity ^@ Belongs to the 2-oxoacid dehydrogenase family. http://togogenome.org/gene/300852:TTH_RS05375 ^@ http://purl.uniprot.org/uniprot/Q5SMH2 ^@ Cofactor|||Function|||PTM|||Similarity ^@ Activated by phosphorylation.|||Belongs to the phosphohexose mutase family.|||Binds 1 Mg(2+) ion per subunit.|||Catalyzes the conversion of glucosamine-6-phosphate to glucosamine-1-phosphate. http://togogenome.org/gene/300852:TTH_RS03210 ^@ http://purl.uniprot.org/uniprot/Q5SKM7 ^@ Function|||Similarity|||Subunit ^@ ATP-dependent specificity component of the Clp protease. It directs the protease to specific substrates. Can perform chaperone functions in the absence of ClpP.|||Belongs to the ClpX chaperone family.|||Component of the ClpX-ClpP complex. Forms a hexameric ring that, in the presence of ATP, binds to fourteen ClpP subunits assembled into a disk-like structure with a central cavity, resembling the structure of eukaryotic proteasomes. http://togogenome.org/gene/300852:TTH_RS04565 ^@ http://purl.uniprot.org/uniprot/Q5SJW6 ^@ Similarity ^@ Belongs to the UDP-galactopyranose/dTDP-fucopyranose mutase family. http://togogenome.org/gene/300852:TTH_RS10175 ^@ http://purl.uniprot.org/uniprot/Q53WC6 ^@ Subcellular Location Annotation ^@ Membrane http://togogenome.org/gene/300852:TTH_RS01710 ^@ http://purl.uniprot.org/uniprot/Q5SLG9 ^@ Cofactor|||Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the phosphopentomutase family.|||Binds 1 or 2 manganese ions.|||Cytoplasm|||Phosphotransfer between the C1 and C5 carbon atoms of pentose. http://togogenome.org/gene/300852:TTH_RS04575 ^@ http://purl.uniprot.org/uniprot/Q5SJW4 ^@ Similarity ^@ Belongs to the peptidase C15 family. http://togogenome.org/gene/300852:TTH_RS06285 ^@ http://purl.uniprot.org/uniprot/Q5SIX6 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the binding-protein-dependent transport system permease family.|||Cell membrane http://togogenome.org/gene/300852:TTH_RS06685 ^@ http://purl.uniprot.org/uniprot/Q5SIP9 ^@ Similarity ^@ Belongs to the peptidase S1C family. http://togogenome.org/gene/300852:TTH_RS01905 ^@ http://purl.uniprot.org/uniprot/Q5SLC9 ^@ Domain|||Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ ATPase component of the type IV pilus (T4P) that plays a role in surface and host cell adhesion, colonization, biofilm maturation, virulence, and twitching, a form of surface-associated motility facilitated by cycles of extension, adhesion, and retraction of T4P fibers (By similarity) (PubMed:29717025). Acts as a molecular motor to provide the energy that is required for biogenesis of the pilus and the extrusion of substrates generated in the cytoplasm (PubMed:27667690). PilB ATPase activity is also essential for T4P extension while antagonist PilT ATPase activity is required for T4P retraction (By similarity).|||Belongs to the GSP E family.|||Cytoplasm|||Homohexamer (PubMed:29717025, PubMed:27667690). Interacts with PilC (By similarity).|||The ATP-binding site is essential for assembly of pili. http://togogenome.org/gene/300852:TTH_RS05365 ^@ http://purl.uniprot.org/uniprot/P52028 ^@ Similarity ^@ Belongs to the DNA polymerase type-A family. http://togogenome.org/gene/300852:TTH_RS03730 ^@ http://purl.uniprot.org/uniprot/Q5SKC5 ^@ Activity Regulation|||Function|||Miscellaneous|||Similarity|||Subunit ^@ Belongs to the uracil-DNA glycosylase (UDG) superfamily. Type 4 (UDGa) family.|||Monomer.|||Product-inhibited by apurinic/apyrimidinic sites.|||Removes uracil bases that are present in DNA as a result of either deamination of cytosine or misincorporation of dUMP instead of dTMP. Can remove uracil from double-stranded DNA containing either a U/G, U/A, U/C or U/T base pair as well as from single-stranded DNA (PubMed:12000829, PubMed:14556741). Specifically recognizes uracil that is flipped out from double-stranded DNA (PubMed:14556741).|||The 4Fe-4S cluster may have a structural rather than a catalytic role. Does not appear to be essential for enzyme activity. http://togogenome.org/gene/300852:TTH_RS09130 ^@ http://purl.uniprot.org/uniprot/Q5SHD2 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the CitM (TC 2.A.11) transporter family.|||Cell membrane|||Membrane http://togogenome.org/gene/300852:TTH_RS02940 ^@ http://purl.uniprot.org/uniprot/Q5SKS9 ^@ Subcellular Location Annotation ^@ Cytoplasm http://togogenome.org/gene/300852:TTH_RS03760 ^@ http://purl.uniprot.org/uniprot/Q5SKB9 ^@ Similarity ^@ Belongs to the peptidase S8 family. http://togogenome.org/gene/300852:TTH_RS00305 ^@ http://purl.uniprot.org/uniprot/Q5SM85 ^@ Function|||Similarity ^@ Belongs to the HrcA family.|||Negative regulator of class I heat shock genes (grpE-dnaK-dnaJ and groELS operons). Prevents heat-shock induction of these operons. http://togogenome.org/gene/300852:TTH_RS01810 ^@ http://purl.uniprot.org/uniprot/Q5SLE9 ^@ Cofactor|||Similarity ^@ Belongs to the Fur family.|||Binds 1 zinc ion per subunit. http://togogenome.org/gene/300852:TTH_RS07260 ^@ http://purl.uniprot.org/uniprot/Q5SIE0 ^@ Similarity ^@ Belongs to the enoyl-CoA hydratase/isomerase family. http://togogenome.org/gene/300852:TTH_RS03355 ^@ http://purl.uniprot.org/uniprot/Q5SKK1 ^@ Similarity ^@ Belongs to the bacterial sugar transferase family. http://togogenome.org/gene/300852:TTH_RS02625 ^@ http://purl.uniprot.org/uniprot/Q5SKZ0 ^@ Cofactor|||Function ^@ Binds 2 [4Fe-4S] clusters.|||Component of a complex that catalyzes the oxidation of glycolate to glyoxylate. http://togogenome.org/gene/300852:TTH_RS05865 ^@ http://purl.uniprot.org/uniprot/Q5SJ58 ^@ Activity Regulation|||Function|||Similarity|||Subunit ^@ Belongs to the trans-sulfuration enzymes family.|||Catalyzes the conversion of O-acetyl-L-homoserine (OAH) into homocysteine in the methionine biosynthesis pathway. Has weak activity with O-acetyl-L-serine, O-phospho-L-serine, L-serine, O-succinyl-L-homoserine and L-homoserine. Shows a very low CTT gamma-synthase activity.|||Homotetramer.|||Inhibited by the carbonyl reagents hydroxylamine and phenylhydrazine. Also inhibited by methionine and propargylglycine. http://togogenome.org/gene/300852:TTH_RS02335 ^@ http://purl.uniprot.org/uniprot/Q5SL44 ^@ Subcellular Location Annotation ^@ Membrane http://togogenome.org/gene/300852:TTH_RS09960 ^@ http://purl.uniprot.org/uniprot/P21777 ^@ Activity Regulation|||Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Allosterically activated by ADP and other diphosphonucleosides (By similarity). Allosterically inhibited by phosphoenolpyruvate which induces the dissociation of the active tetramer into an inactive two-subunit forms.|||Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade 'B1' sub-subfamily.|||Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.|||Cytoplasm|||Homotetramer. http://togogenome.org/gene/300852:TTH_RS04755 ^@ http://purl.uniprot.org/uniprot/Q5SJS4 ^@ Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily.|||Cytoplasm|||Homodimer. http://togogenome.org/gene/300852:TTH_RS00985 ^@ http://purl.uniprot.org/uniprot/Q5SLV5 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the NDK family.|||Cytoplasm|||Homotetramer.|||Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. http://togogenome.org/gene/300852:TTH_RS08520 ^@ http://purl.uniprot.org/uniprot/Q5SHP6 ^@ Function|||Similarity|||Subunit ^@ Belongs to the universal ribosomal protein uL29 family.|||One of the proteins that surrounds the polypeptide exit tunnel on the outside of the subunit.|||Part of the 50S ribosomal subunit. http://togogenome.org/gene/300852:TTH_RS05320 ^@ http://purl.uniprot.org/uniprot/Q5SJG3 ^@ Cofactor|||Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the endoribonuclease YbeY family.|||Binds 1 zinc ion.|||Cytoplasm|||Single strand-specific metallo-endoribonuclease involved in late-stage 70S ribosome quality control and in maturation of the 3' terminus of the 16S rRNA. http://togogenome.org/gene/300852:TTH_RS02830 ^@ http://purl.uniprot.org/uniprot/P56206 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the class-II aminoacyl-tRNA synthetase family.|||Catalyzes the attachment of glycine to tRNA(Gly).|||Cytoplasm|||Homodimer. http://togogenome.org/gene/300852:TTH_RS07985 ^@ http://purl.uniprot.org/uniprot/Q5SI03 ^@ Similarity ^@ Belongs to the Glu/Leu/Phe/Val dehydrogenases family. http://togogenome.org/gene/300852:TTH_RS09800 ^@ http://purl.uniprot.org/uniprot/Q5SH01 ^@ Subcellular Location Annotation ^@ Membrane http://togogenome.org/gene/300852:TTH_RS00510 ^@ http://purl.uniprot.org/uniprot/Q5SM45 ^@ Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the class-I aminoacyl-tRNA synthetase family.|||Cytoplasm|||Monomer. http://togogenome.org/gene/300852:TTH_RS09950 ^@ http://purl.uniprot.org/uniprot/Q5SGX1 ^@ Cofactor|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 1 subfamily.|||Binds 2 magnesium ions per tetramer.|||Cytoplasm|||Tetramer of two alpha and two beta subunits. http://togogenome.org/gene/300852:TTH_RS05335 ^@ http://purl.uniprot.org/uniprot/Q5SJG0 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the flotillin-like FloA family.|||Cell membrane|||Found in functional membrane microdomains (FMM) that may be equivalent to eukaryotic membrane rafts. FMMs are highly dynamic and increase in number as cells age. Flotillins are thought to be important factors in membrane fluidity.|||Homooligomerizes.|||Membrane raft http://togogenome.org/gene/300852:TTH_RS00300 ^@ http://purl.uniprot.org/uniprot/Q5SM86 ^@ Similarity ^@ Belongs to the P(II) protein family. http://togogenome.org/gene/300852:TTH_RS10370 ^@ http://purl.uniprot.org/uniprot/Q53W86 ^@ Subcellular Location Annotation ^@ Cell inner membrane|||Membrane http://togogenome.org/gene/300852:TTH_RS01090 ^@ http://purl.uniprot.org/uniprot/Q8VVE3 ^@ Caution|||Function|||PTM|||Similarity|||Subunit ^@ Belongs to the universal ribosomal protein uL10 family.|||Forms part of the ribosomal stalk, playing a central role in the interaction of the ribosome with GTP-bound translation factors.|||Note that in PubMed:11154066 it was found that the N-terminus is blocked, whereas the mass determined in PubMed:16287167 suggests that the initiator methionine is removed. No extra mass for a blocking group was found.|||Part of the 50S ribosomal subunit. Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. Forms a heptameric L10(L12)2(L12)2(L12)2 complex, where L10 forms an elongated spine to which 3 L12 dimers bind in a sequential fashion.|||The N-terminus is blocked. http://togogenome.org/gene/300852:TTH_RS01015 ^@ http://purl.uniprot.org/uniprot/Q5SLU9 ^@ Subcellular Location Annotation ^@ Membrane http://togogenome.org/gene/300852:TTH_RS07110 ^@ http://purl.uniprot.org/uniprot/Q5SIG5 ^@ Similarity ^@ Belongs to the CcmF/CycK/Ccl1/NrfE/CcsA family. http://togogenome.org/gene/300852:TTH_RS08485 ^@ http://purl.uniprot.org/uniprot/P0DOY8 ^@ Function|||Similarity|||Subunit ^@ Belongs to the universal ribosomal protein uL6 family.|||Part of the 50S ribosomal subunit.|||This protein binds to the 23S rRNA, and is important in its secondary structure. It is located near the subunit interface in the base of the L7/L12 stalk, and near the tRNA binding site of the peptidyltransferase center. http://togogenome.org/gene/300852:TTH_RS02885 ^@ http://purl.uniprot.org/uniprot/P60491 ^@ Function|||Similarity ^@ Belongs to the bacterial ribosomal protein bL20 family.|||Binds directly to 23S ribosomal RNA and is necessary for the in vitro assembly process of the 50S ribosomal subunit. It is not involved in the protein synthesizing functions of that subunit (By similarity). http://togogenome.org/gene/300852:TTH_RS02745 ^@ http://purl.uniprot.org/uniprot/Q5SKW6 ^@ Similarity ^@ Belongs to the ribF family. http://togogenome.org/gene/300852:TTH_RS03480 ^@ http://purl.uniprot.org/uniprot/Q5SKH6 ^@ Similarity ^@ Belongs to the precorrin methyltransferase family. http://togogenome.org/gene/300852:TTH_RS11115 ^@ http://purl.uniprot.org/uniprot/Q53VV6 ^@ Function|||Similarity|||Subunit ^@ Belongs to the CRISPR-associated endoribonuclease Cas2 protein family.|||CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Functions as a ssRNA-specific endoribonuclease. Involved in the integration of spacer DNA into the CRISPR cassette.|||Homodimer, forms a heterotetramer with a Cas1 homodimer. http://togogenome.org/gene/300852:TTH_RS10945 ^@ http://purl.uniprot.org/uniprot/Q53VY2 ^@ Activity Regulation|||Cofactor|||Domain|||Function|||Similarity ^@ CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas3 plus Cascade participate in CRISPR interference, the third stage of CRISPR immunity. The N-terminal domain (residues 6-260) acts as a ssDNA endonuclease, has no activity on dsDNA.|||In the N-terminal section; belongs to the CRISPR-associated nuclease Cas3-HD family.|||In the central section; belongs to the CRISPR-associated helicase Cas3 family.|||Inhibited by EDTA, Mg(2+) and Ca(2+).|||Proteins of this family have an N-terminal nuclease domain and a C-terminal helicase/ATPase domain. In some CRISPR/Cas systems the domains are swapped, in others they are encoded separately.|||The ssDNA endonuclease activity (residues 6-260) is stimulated by Mn(2+), Co(2+), Ni(2+), Cu(2+) and Zn(2+), but not by Mg(2+) or Ca(2+). A Ni(2+) ion is seen in crystals upon soaking. http://togogenome.org/gene/300852:TTH_RS10505 ^@ http://purl.uniprot.org/uniprot/Q53W59 ^@ Similarity ^@ Belongs to the cytochrome P450 family. http://togogenome.org/gene/300852:TTH_RS01965 ^@ http://purl.uniprot.org/uniprot/Q5SLC0 ^@ Similarity ^@ Belongs to the UbiD family. http://togogenome.org/gene/300852:TTH_RS09705 ^@ http://purl.uniprot.org/uniprot/Q5SH16 ^@ Function|||Similarity ^@ Belongs to the alpha-IPM synthase/homocitrate synthase family. Homocitrate synthase LYS20/LYS21 subfamily.|||Catalyzes the aldol-type condensation of 2-oxoglutarate with acetyl-CoA to yield homocitrate. Carries out the first step of the alpha-aminoadipate (AAA) lysine biosynthesis pathway. http://togogenome.org/gene/300852:TTH_RS10995 ^@ http://purl.uniprot.org/uniprot/Q53WG4 ^@ Similarity ^@ Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. http://togogenome.org/gene/300852:TTH_RS07465 ^@ http://purl.uniprot.org/uniprot/Q5SIA0 ^@ Subcellular Location Annotation ^@ Membrane http://togogenome.org/gene/300852:TTH_RS03685 ^@ http://purl.uniprot.org/uniprot/P54263 ^@ Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the class-II aminoacyl-tRNA synthetase family.|||Cytoplasm|||Homodimer. http://togogenome.org/gene/300852:TTH_RS01005 ^@ http://purl.uniprot.org/uniprot/Q5SLV1 ^@ Similarity ^@ Belongs to the universal stress protein A family. http://togogenome.org/gene/300852:TTH_RS00395 ^@ http://purl.uniprot.org/uniprot/Q5SM67 ^@ Similarity ^@ Belongs to the TrpF family. http://togogenome.org/gene/300852:TTH_RS02915 ^@ http://purl.uniprot.org/uniprot/Q5SKT4 ^@ Similarity ^@ Belongs to the thiolase-like superfamily. Thiolase family. http://togogenome.org/gene/300852:TTH_RS02850 ^@ http://purl.uniprot.org/uniprot/Q5SKU7 ^@ Similarity ^@ Belongs to the TlyA family. http://togogenome.org/gene/300852:TTH_RS02800 ^@ http://purl.uniprot.org/uniprot/Q5SKV6 ^@ Function|||Similarity|||Subunit ^@ Belongs to the succinate/malate CoA ligase alpha subunit family.|||Heterotetramer of two alpha and two beta subunits.|||Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The alpha subunit of the enzyme binds the substrates coenzyme A and phosphate, while succinate binding and nucleotide specificity is provided by the beta subunit. http://togogenome.org/gene/300852:TTH_RS07265 ^@ http://purl.uniprot.org/uniprot/Q5SID9 ^@ Similarity ^@ Belongs to the PNP/UDP phosphorylase family. http://togogenome.org/gene/300852:TTH_RS09775 ^@ http://purl.uniprot.org/uniprot/Q5SH04 ^@ Similarity ^@ Belongs to the Cob(I)alamin adenosyltransferase family. http://togogenome.org/gene/300852:TTH_RS07205 ^@ http://purl.uniprot.org/uniprot/Q5SME3 ^@ Function|||PTM ^@ Binds 1 heme c group covalently per subunit.|||This monoheme basic protein appears to function as an electron donor to cytochrome oxidase in T.thermophilus. http://togogenome.org/gene/300852:TTH_RS00930 ^@ http://purl.uniprot.org/uniprot/P03942 ^@ Cofactor|||Function ^@ Binds 1 [3Fe-4S] cluster.|||Binds 1 [4Fe-4S] cluster.|||Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. http://togogenome.org/gene/300852:TTH_RS09275 ^@ http://purl.uniprot.org/uniprot/Q5SHA3 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the binding-protein-dependent transport system permease family.|||Cell membrane http://togogenome.org/gene/300852:TTH_RS07390 ^@ http://purl.uniprot.org/uniprot/Q5SIB4 ^@ Subcellular Location Annotation ^@ Membrane http://togogenome.org/gene/300852:TTH_RS09685 ^@ http://purl.uniprot.org/uniprot/Q5SH20 ^@ Function|||Similarity|||Subunit ^@ Belongs to the LeuD family. LeuD type 2 subfamily.|||Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.|||Heterodimer of LeuC and LeuD. http://togogenome.org/gene/300852:TTH_RS00800 ^@ http://purl.uniprot.org/uniprot/Q5SLZ1 ^@ Similarity ^@ Belongs to the AOR/FOR family. http://togogenome.org/gene/300852:TTH_RS07770 ^@ http://purl.uniprot.org/uniprot/P33197 ^@ Cofactor|||Similarity|||Subunit ^@ Belongs to the isocitrate and isopropylmalate dehydrogenases family.|||Binds 1 Mg(2+) or Mn(2+) ion per subunit.|||Homodimer. http://togogenome.org/gene/300852:TTH_RS01060 ^@ http://purl.uniprot.org/uniprot/Q5SLU0 ^@ Subcellular Location Annotation ^@ Membrane http://togogenome.org/gene/300852:TTH_RS08510 ^@ http://purl.uniprot.org/uniprot/Q5SHP8 ^@ Function|||Similarity|||Subunit ^@ Belongs to the universal ribosomal protein uL14 family.|||Contacts the 16S rRNA of the 30S subunit in two different positions helping to form bridges B5 and B8.|||Part of the 50S ribosomal subunit. Contacts protein L19. Forms a bridge to the 30S subunit in the 70S ribosome, contacting the 16S rRNA.|||This protein binds directly to 23S ribosomal RNA. http://togogenome.org/gene/300852:TTH_RS08960 ^@ http://purl.uniprot.org/uniprot/Q5SHG3 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Acetyl-CoA carboxylase is a heterohexamer composed of biotin carboxyl carrier protein (AccB), biotin carboxylase (AccC) and two subunits each of ACCase subunit alpha (AccA) and ACCase subunit beta (AccD).|||Belongs to the AccA family.|||Component of the acetyl coenzyme A carboxylase (ACC) complex. First, biotin carboxylase catalyzes the carboxylation of biotin on its carrier protein (BCCP) and then the CO(2) group is transferred by the carboxyltransferase to acetyl-CoA to form malonyl-CoA.|||Cytoplasm http://togogenome.org/gene/300852:TTH_RS09035 ^@ http://purl.uniprot.org/uniprot/P60493 ^@ Function|||Similarity|||Subunit ^@ Belongs to the bacterial ribosomal protein bL27 family.|||Found on the solvent side of the large subunit.|||Part of the 50S ribosomal subunit. http://togogenome.org/gene/300852:TTH_RS01845 ^@ http://purl.uniprot.org/uniprot/Q5SLE2 ^@ Subcellular Location Annotation ^@ Membrane http://togogenome.org/gene/300852:TTH_RS02675 ^@ http://purl.uniprot.org/uniprot/Q5SKY0 ^@ Function|||Similarity|||Subunit ^@ Belongs to the PurK/PurT family.|||Catalyzes the ATP-dependent conversion of 5-aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5-carboxyaminoimidazole ribonucleotide (N5-CAIR).|||Catalyzes the ATP-dependent conversion of 5-aminoimidazole ribonucleotide (AIR) and HCO(3)- to N5-carboxyaminoimidazole ribonucleotide (N5-CAIR).|||Homodimer. http://togogenome.org/gene/300852:TTH_RS09730 ^@ http://purl.uniprot.org/uniprot/Q5SH10 ^@ Cofactor|||Similarity|||Subunit ^@ Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family.|||Binds 1 FAD per subunit.|||Homodimer. http://togogenome.org/gene/300852:TTH_RS01250 ^@ http://purl.uniprot.org/uniprot/Q5SLQ4 ^@ Cofactor|||Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the radical SAM superfamily. Lipoyl synthase family.|||Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.|||Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.|||Cytoplasm http://togogenome.org/gene/300852:TTH_RS09595 ^@ http://purl.uniprot.org/uniprot/Q56243 ^@ Domain|||Function|||Miscellaneous|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the UvrB family.|||Cytoplasm|||Forms a heterotetramer with UvrA during the search for lesions. Interacts with UvrC in an incision complex (By similarity).|||The ATPase activity is stimulated by single-stranded DNA. UvrB is stable up to 80 Celsius degrees.|||The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA(2)B(2) complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage (By similarity).|||The beta-hairpin motif is involved in DNA binding. http://togogenome.org/gene/300852:TTH_RS09700 ^@ http://purl.uniprot.org/uniprot/Q5SH17 ^@ Similarity ^@ Belongs to the HicA mRNA interferase family. http://togogenome.org/gene/300852:TTH_RS01415 ^@ http://purl.uniprot.org/uniprot/Q5SLM6 ^@ Similarity ^@ Belongs to the flavin oxidoreductase frp family. http://togogenome.org/gene/300852:TTH_RS01225 ^@ http://purl.uniprot.org/uniprot/Q5SJJ0 ^@ Function|||Similarity ^@ Belongs to the transposase mutator family.|||Required for the transposition of the insertion element. http://togogenome.org/gene/300852:TTH_RS06040 ^@ http://purl.uniprot.org/uniprot/Q5SJ25 ^@ Similarity ^@ Belongs to the MreC family. http://togogenome.org/gene/300852:TTH_RS05910 ^@ http://purl.uniprot.org/uniprot/Q5SJ49 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the HisA/HisF family.|||Cytoplasm http://togogenome.org/gene/300852:TTH_RS07055 ^@ http://purl.uniprot.org/uniprot/Q5SIH6 ^@ Cofactor|||Function|||Similarity|||Subunit ^@ Belongs to the NnrD/CARKD family.|||Belongs to the NnrE/AIBP family.|||Bifunctional enzyme that catalyzes the epimerization of the S- and R-forms of NAD(P)HX and the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. This allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.|||Binds 1 potassium ion per subunit.|||Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.|||Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.|||Homotetramer.|||In the C-terminal section; belongs to the NnrD/CARKD family.|||In the N-terminal section; belongs to the NnrE/AIBP family. http://togogenome.org/gene/300852:TTH_RS07565 ^@ http://purl.uniprot.org/uniprot/Q5SI80 ^@ Similarity ^@ Belongs to the peptidase S49 family. http://togogenome.org/gene/300852:TTH_RS10720 ^@ http://purl.uniprot.org/uniprot/Q53W24 ^@ Activity Regulation|||Caution|||Function|||Similarity ^@ Belongs to the FGGY kinase family.|||Inhibited by fructose 1,6-bisphosphate (FBP).|||Key enzyme in the regulation of glycerol uptake and metabolism. Catalyzes the phosphorylation of glycerol to yield sn-glycerol 3-phosphate.|||Lacks conserved residue(s) required for the propagation of feature annotation. http://togogenome.org/gene/300852:TTH_RS01745 ^@ http://purl.uniprot.org/uniprot/Q5SLG2 ^@ Similarity ^@ Belongs to the PHP hydrolase family. HisK subfamily. http://togogenome.org/gene/300852:TTH_RS00475 ^@ http://purl.uniprot.org/uniprot/Q60019 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the complex I subunit 1 family.|||Cell inner membrane|||NDH-1 is composed of 15 different subunits, Nqo1 to Nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits Nqo7, Nqo8 and Nqo10 to Nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits Nqo1 to Nqo6, Nqo9 and Nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers.|||NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP. http://togogenome.org/gene/300852:TTH_RS07415 ^@ http://purl.uniprot.org/uniprot/P60488 ^@ Function|||Similarity|||Subunit ^@ Belongs to the universal ribosomal protein uL13 family.|||Part of the 50S ribosomal subunit.|||This protein is one of the early assembly proteins of the 50S ribosomal subunit, although it is not seen to bind rRNA by itself. It is important during the early stages of 50S assembly. http://togogenome.org/gene/300852:TTH_RS07030 ^@ http://purl.uniprot.org/uniprot/Q5SII1 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the P-Pant transferase superfamily. AcpS family.|||Cytoplasm|||Transfers the 4'-phosphopantetheine moiety from coenzyme A to a Ser of acyl-carrier-protein. http://togogenome.org/gene/300852:TTH_RS04075 ^@ http://purl.uniprot.org/uniprot/Q5SMG6 ^@ Similarity ^@ Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. http://togogenome.org/gene/300852:TTH_RS07120 ^@ http://purl.uniprot.org/uniprot/Q5SIG3 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the CcmC/CycZ/HelC family.|||Membrane http://togogenome.org/gene/300852:TTH_RS05715 ^@ http://purl.uniprot.org/uniprot/Q5SJ90 ^@ Function ^@ This protein is a component of the acetyl coenzyme A carboxylase complex; first, biotin carboxylase catalyzes the carboxylation of the carrier protein and then the transcarboxylase transfers the carboxyl group to form malonyl-CoA. http://togogenome.org/gene/300852:TTH_RS02465 ^@ http://purl.uniprot.org/uniprot/Q5SL18 ^@ Similarity ^@ Belongs to the histone deacetylase family. http://togogenome.org/gene/300852:TTH_RS02735 ^@ http://purl.uniprot.org/uniprot/Q5SKW8 ^@ Function|||Similarity|||Subunit ^@ Belongs to the GcvP family. N-terminal subunit subfamily.|||The glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO(2) is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein.|||The glycine cleavage system is composed of four proteins: P, T, L and H. In this organism, the P 'protein' is a heterodimer of two subunits. http://togogenome.org/gene/300852:TTH_RS05700 ^@ http://purl.uniprot.org/uniprot/Q5SJ93 ^@ Function|||Similarity ^@ Belongs to the NusB family.|||Involved in transcription antitermination. Required for transcription of ribosomal RNA (rRNA) genes. Binds specifically to the boxA antiterminator sequence of the ribosomal RNA (rrn) operons. http://togogenome.org/gene/300852:TTH_RS01620 ^@ http://purl.uniprot.org/uniprot/Q5SLI4 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the autoinducer-2 exporter (AI-2E) (TC 2.A.86) family.|||Membrane http://togogenome.org/gene/300852:TTH_RS10075 ^@ http://purl.uniprot.org/uniprot/Q53WH7 ^@ Similarity ^@ Belongs to the acyl-CoA dehydrogenase family. http://togogenome.org/gene/300852:TTH_RS08850 ^@ http://purl.uniprot.org/uniprot/Q5SHI4 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the PlsX family.|||Catalyzes the reversible formation of acyl-phosphate (acyl-PO(4)) from acyl-[acyl-carrier-protein] (acyl-ACP). This enzyme utilizes acyl-ACP as fatty acyl donor, but not acyl-CoA.|||Cytoplasm|||Homodimer. Probably interacts with PlsY. http://togogenome.org/gene/300852:TTH_RS07880 ^@ http://purl.uniprot.org/uniprot/P29253 ^@ Cofactor|||Function|||Similarity|||Subunit ^@ Belongs to the RNase H family.|||Binds 1 Mg(2+) ion per subunit. May bind a second metal ion at a regulatory site, or after substrate binding.|||Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.|||Monomer. http://togogenome.org/gene/300852:TTH_RS01460 ^@ http://purl.uniprot.org/uniprot/Q5SLL6 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the GPI family.|||Catalyzes the reversible isomerization of glucose-6-phosphate to fructose-6-phosphate.|||Cytoplasm http://togogenome.org/gene/300852:TTH_RS04840 ^@ http://purl.uniprot.org/uniprot/Q5SJQ6 ^@ Function|||Similarity ^@ Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.|||Catalyzes the reversible oxidation of 3-phospho-D-glycerate to 3-phosphonooxypyruvate, the first step of the phosphorylated L-serine biosynthesis pathway. Also catalyzes the reversible oxidation of 2-hydroxyglutarate to 2-oxoglutarate. http://togogenome.org/gene/300852:TTH_RS08690 ^@ http://purl.uniprot.org/uniprot/Q5SHL5 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the MscS (TC 1.A.23) family.|||Membrane http://togogenome.org/gene/300852:TTH_RS03540 ^@ http://purl.uniprot.org/uniprot/Q5SKG4 ^@ Subcellular Location Annotation ^@ Membrane http://togogenome.org/gene/300852:TTH_RS00560 ^@ http://purl.uniprot.org/uniprot/Q5SM36 ^@ Subcellular Location Annotation ^@ Membrane http://togogenome.org/gene/300852:TTH_RS05505 ^@ http://purl.uniprot.org/uniprot/Q5SJD3 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the glycosyltransferase 4 family. MraY subfamily.|||Membrane http://togogenome.org/gene/300852:TTH_RS10425 ^@ http://purl.uniprot.org/uniprot/Q53W75 ^@ Similarity ^@ Belongs to the glycosyl hydrolase 1 family. http://togogenome.org/gene/300852:TTH_RS02235 ^@ http://purl.uniprot.org/uniprot/Q5SL64 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the imidazoleglycerol-phosphate dehydratase family.|||Cytoplasm http://togogenome.org/gene/300852:TTH_RS01430 ^@ http://purl.uniprot.org/uniprot/Q5SLM3 ^@ Cofactor|||Function|||Similarity|||Subunit ^@ Belongs to the peptidase M32 family.|||Binds 1 zinc ion per subunit.|||Broad specificity carboxypetidase that releases amino acids sequentially from the C-terminus, including neutral, aromatic, polar and basic residues, but not Pro. Has lower activity with substrates ending with Gly or Glu.|||Homodimer. http://togogenome.org/gene/300852:TTH_RS02935 ^@ http://purl.uniprot.org/uniprot/Q5SKT0 ^@ Function|||Similarity ^@ Belongs to the pyridoxal phosphate-binding protein YggS/PROSC family.|||Pyridoxal 5'-phosphate (PLP)-binding protein, which is involved in PLP homeostasis. http://togogenome.org/gene/300852:TTH_RS08265 ^@ http://purl.uniprot.org/uniprot/Q5SHU7 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the binding-protein-dependent transport system permease family.|||Cell membrane|||Membrane http://togogenome.org/gene/300852:TTH_RS09015 ^@ http://purl.uniprot.org/uniprot/Q5SHF2 ^@ Similarity ^@ Belongs to the LytR/CpsA/Psr (LCP) family. http://togogenome.org/gene/300852:TTH_RS06825 ^@ http://purl.uniprot.org/uniprot/P19436 ^@ Function|||Similarity|||Subunit ^@ Belongs to the bacterial histone-like protein family.|||Has been isolated in complexes with 5S rRNA and bL25, and with 5S rRNA, bL25 and uL5 (Ref.3). Homodimer.|||Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions. http://togogenome.org/gene/300852:TTH_RS03390 ^@ http://purl.uniprot.org/uniprot/Q5SKJ3 ^@ Similarity ^@ Belongs to the phosphohexose mutase family. http://togogenome.org/gene/300852:TTH_RS10015 ^@ http://purl.uniprot.org/uniprot/Q9X9D5 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the DnaA family.|||Cytoplasm|||Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box): 5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phospholipids.|||Some 16-18 DnaA protein molecules bind their sites in oriC. http://togogenome.org/gene/300852:TTH_RS04595 ^@ http://purl.uniprot.org/uniprot/Q5SJW1 ^@ Similarity ^@ Belongs to the thiolase-like superfamily. Thiolase family. http://togogenome.org/gene/300852:TTH_RS06245 ^@ http://purl.uniprot.org/uniprot/Q9ZND4 ^@ Function|||Similarity|||Subunit ^@ Belongs to the LeuD family. LeuD type 1 subfamily.|||Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.|||Heterodimer of LeuC and LeuD. http://togogenome.org/gene/300852:TTH_RS03765 ^@ http://purl.uniprot.org/uniprot/Q5SMF7 ^@ Similarity ^@ Belongs to the LytR/CpsA/Psr (LCP) family. http://togogenome.org/gene/300852:TTH_RS00770 ^@ http://purl.uniprot.org/uniprot/Q5SLZ7 ^@ Similarity ^@ Belongs to the class-II aminoacyl-tRNA synthetase family. http://togogenome.org/gene/300852:TTH_RS00010 ^@ http://purl.uniprot.org/uniprot/Q5SME1 ^@ Activity Regulation|||Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the enolase family.|||Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis.|||Cell surface|||Cytoplasm|||Secreted|||The covalent binding to the substrate causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein. http://togogenome.org/gene/300852:TTH_RS09470 ^@ http://purl.uniprot.org/uniprot/Q5SIU5 ^@ Function|||Similarity ^@ Belongs to the transposase mutator family.|||Required for the transposition of the insertion element. http://togogenome.org/gene/300852:TTH_RS11655 ^@ http://purl.uniprot.org/uniprot/Q5SL15 ^@ Similarity ^@ Belongs to the glycosyl hydrolase 13 family. TreS subfamily. http://togogenome.org/gene/300852:TTH_RS09865 ^@ http://purl.uniprot.org/uniprot/Q5SGY8 ^@ Similarity ^@ Belongs to the SCO1/2 family. http://togogenome.org/gene/300852:TTH_RS01800 ^@ http://purl.uniprot.org/uniprot/Q5SLF1 ^@ Similarity ^@ Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. http://togogenome.org/gene/300852:TTH_RS04455 ^@ http://purl.uniprot.org/uniprot/Q5SJZ0 ^@ Similarity ^@ Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. http://togogenome.org/gene/300852:TTH_RS02245 ^@ http://purl.uniprot.org/uniprot/Q5SL62 ^@ Similarity ^@ Belongs to the carbohydrate kinase PfkB family. http://togogenome.org/gene/300852:TTH_RS05785 ^@ http://purl.uniprot.org/uniprot/Q5SJ75 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the polyribonucleotide nucleotidyltransferase family.|||Cytoplasm|||Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction. http://togogenome.org/gene/300852:TTH_RS01690 ^@ http://purl.uniprot.org/uniprot/Q5SLH3 ^@ Function|||Similarity ^@ Attaches a formyl group to the free amino group of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by promoting its recognition by IF2 and preventing the misappropriation of this tRNA by the elongation apparatus.|||Belongs to the Fmt family. http://togogenome.org/gene/300852:TTH_RS02965 ^@ http://purl.uniprot.org/uniprot/Q5SKS4 ^@ Cofactor|||Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the zinc-containing alcohol dehydrogenase family.|||Binds 2 Zn(2+) ions per subunit. Contains one structural ion and one catalytic ion that may be less tightly bound at the site.|||Catalyzes the NAD(+)-dependent oxidation of L-threonine to 2-amino-3-ketobutyrate.|||Cytoplasm|||Homotetramer. http://togogenome.org/gene/300852:TTH_RS06240 ^@ http://purl.uniprot.org/uniprot/Q9ZND5 ^@ Cofactor|||Function|||Similarity|||Subunit ^@ Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily.|||Binds 1 [4Fe-4S] cluster per subunit.|||Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.|||Heterodimer of LeuC and LeuD. http://togogenome.org/gene/300852:TTH_RS02870 ^@ http://purl.uniprot.org/uniprot/Q5SKU3 ^@ Similarity|||Subunit ^@ Belongs to the enoyl-CoA hydratase/isomerase family.|||Homohexamer; dimer of trimers. http://togogenome.org/gene/300852:TTH_RS08515 ^@ http://purl.uniprot.org/uniprot/P0DOY7 ^@ Disruption Phenotype|||Function|||Similarity|||Subunit ^@ Belongs to the universal ribosomal protein uS17 family.|||Increased generation time and a temperature-sensitive phenotype.|||One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it helps nucleate assembly of the platform and body of the 30S subunit by bringing together and stabilizing interactions between several different RNA helices. The combined cluster of proteins S8, S12 and S17 appears to hold together the shoulder and platform of the 30S subunit.|||Part of the 30S ribosomal subunit. Contacts protein S12. http://togogenome.org/gene/300852:TTH_RS04460 ^@ http://purl.uniprot.org/uniprot/Q5SJY9 ^@ Similarity ^@ Belongs to the Nudix hydrolase family. http://togogenome.org/gene/300852:TTH_RS05550 ^@ http://purl.uniprot.org/uniprot/Q5SJC4 ^@ Cofactor|||Domain|||Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the RuvC family.|||Binds 2 Mg(2+) ion per subunit.|||Cytoplasm|||Homodimer which binds Holliday junction (HJ) DNA. The HJ becomes 2-fold symmetrical on binding to RuvC with unstacked arms; it has a different conformation from HJ DNA in complex with RuvA. In the full resolvosome a probable DNA-RuvA(4)-RuvB(12)-RuvC(2) complex forms which resolves the HJ.|||The RuvA-RuvB-RuvC complex processes Holliday junction (HJ) DNA during genetic recombination and DNA repair (Probable). Endonuclease that resolves Holliday junction (HJ) intermediates. Cleaves cruciform DNA by making single-stranded nicks across the HJ at symmetrical positions within the homologous arms, yielding a 5'-phosphate and a 3'-hydroxyl group; requires a central core of homology in the junction. The cleavage reactions can be uncoupled; the presence of a 5'-phosphate in a half-cut site is required for second strand cleavage (PubMed:23118486, PubMed:23980027, PubMed:31506434). The consensus cleavage sequence is 5'-(A/T)TT(C/G)-3'. Cleavage occurs on the 3'-side of the TT dinucleotide at the point of strand exchange (PubMed:31506434).|||The wedge motif stacks with DNA close to the Holliday junction point; Arg-76 interacts with and eventually disrupts the T:A base pair cleavage site, and subsequent to cleavage may prevent their reannealment. http://togogenome.org/gene/300852:TTH_RS03255 ^@ http://purl.uniprot.org/uniprot/Q5SKL8 ^@ Similarity ^@ Belongs to the CapA family. http://togogenome.org/gene/300852:TTH_RS06580 ^@ http://purl.uniprot.org/uniprot/Q5SIR5 ^@ Function|||Similarity|||Subunit ^@ Belongs to the ribose 5-phosphate isomerase family.|||Catalyzes the reversible conversion of ribose-5-phosphate to ribulose 5-phosphate.|||Homodimer. http://togogenome.org/gene/300852:TTH_RS04815 ^@ http://purl.uniprot.org/uniprot/Q5SJR1 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the triosephosphate isomerase family.|||Cytoplasm|||Homodimer.|||Involved in the gluconeogenesis. Catalyzes stereospecifically the conversion of dihydroxyacetone phosphate (DHAP) to D-glyceraldehyde-3-phosphate (G3P). http://togogenome.org/gene/300852:TTH_RS04070 ^@ http://purl.uniprot.org/uniprot/Q5SMG7 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the 4-toluene sulfonate uptake permease (TSUP) (TC 2.A.102) family.|||Cell membrane|||Membrane http://togogenome.org/gene/300852:TTH_RS07570 ^@ http://purl.uniprot.org/uniprot/Q5SI79 ^@ Subcellular Location Annotation ^@ Membrane http://togogenome.org/gene/300852:TTH_RS02390 ^@ http://purl.uniprot.org/uniprot/Q5SL33 ^@ Similarity ^@ Belongs to the Nudix hydrolase family. http://togogenome.org/gene/300852:TTH_RS09060 ^@ http://purl.uniprot.org/uniprot/Q5SHE5 ^@ Cofactor|||Similarity|||Subunit ^@ Belongs to the RtcB family.|||Binds 2 manganese ions per subunit.|||Monomer. http://togogenome.org/gene/300852:TTH_RS08545 ^@ http://purl.uniprot.org/uniprot/P60405 ^@ Function|||Similarity|||Subunit ^@ Belongs to the universal ribosomal protein uL2 family.|||One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity; this is somewhat controversial (By similarity). Makes several contacts with the 16S rRNA (forming bridge B7b) in the 70S ribosome.|||Part of the 50S ribosomal subunit. Forms a bridge to the 30S subunit in the 70S ribosome, contacting the 16S rRNA. May also make transient contacts with protein S6 during translation in the 70S ribosome. http://togogenome.org/gene/300852:TTH_RS05440 ^@ http://purl.uniprot.org/uniprot/Q5SJE5 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the ABC-4 integral membrane protein family. FtsX subfamily.|||Membrane http://togogenome.org/gene/300852:TTH_RS06370 ^@ http://purl.uniprot.org/uniprot/Q5SIV8 ^@ Similarity ^@ Belongs to the UPF0145 family. http://togogenome.org/gene/300852:TTH_RS06875 ^@ http://purl.uniprot.org/uniprot/Q5SIL0 ^@ Disruption Phenotype|||Function|||Induction|||Subunit ^@ Activates transcription. The consensus DNA-binding site of this transcriptional regulator is 5'-WWGTGAN(5-7)ACACWW-3' in which W is A or T and N is G, A, T or C. Regulated genes include those encoding proteins involved in nutrient and energy supply, redox control and polyadenylation of mRNA (PubMed:18699868). Also regulates genes involved in oxidative stress response such as genes encoding manganese superoxide dismutase and catalase, and genes encoding a protein involved in nucleotide excision repair of damaged DNA and putative proteins involved in redox control, protein degradation and transcriptional regulation (PubMed:21054499).|||Homodimer.|||Increased expression during the stationary phase when grown at 70 degrees Celsius (PubMed:18699868). Increased expression during the logarithmic growth phase in oxidative stress and upon treatment with diamide. Increased expression by heavy metal ion, antibiotic, high salt and organic solvent stresses (PubMed:21054499).|||Viable, but has growth defects, particularly when grown in a synthetic medium. Increased sensitivity to disulfide stress. Decreased expression of TTHA0986, TTHA0770, TTHA0337, TTHA1028, TTHA0654, TTHA0655, TTHA0769, TTHA0425, TTHA0634, TTHA0635, TTHA0636, TTHA0637, TTHA0638, TTHA0570, TTHA0030, TTHA0460, TTHB243, TTHA1128, TTHA0035, TTHA0520 and TTHA1803 genes. http://togogenome.org/gene/300852:TTH_RS08095 ^@ http://purl.uniprot.org/uniprot/Q5SHY1 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the YbaB/EbfC family.|||Binds to DNA and alters its conformation. May be involved in regulation of gene expression, nucleoid organization and DNA protection.|||Homodimer.|||nucleoid http://togogenome.org/gene/300852:TTH_RS09965 ^@ http://purl.uniprot.org/uniprot/Q5SGW7 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the methyltransferase superfamily. RsmI family.|||Catalyzes the 2'-O-methylation of the ribose of cytidine 1402 (C1402) in 16S rRNA.|||Cytoplasm http://togogenome.org/gene/300852:TTH_RS06480 ^@ http://purl.uniprot.org/uniprot/Q5SIT6 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the V-ATPase 116 kDa subunit family.|||Membrane http://togogenome.org/gene/300852:TTH_RS04675 ^@ http://purl.uniprot.org/uniprot/Q5SJV1 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the RbfA family.|||Cytoplasm|||Interacts with the 30S ribosomal subunit as a monomer, binding in a position overlapping the sites of the A and P site tRNAs, and displacing segments of the 16S rRNA. Probably contacts 16S rRNA and ribosomal protein S9 and S13.|||One of several proteins that assist in the late maturation steps of the functional core of the 30S ribosomal subunit. Associates with free 30S ribosomal subunits (but not with 30S subunits that are part of 70S ribosomes or polysomes) (PubMed:17996707). Required for efficient processing of 16S rRNA. Probably interacts with the 5'-terminal helix region of 16S rRNA, bringing together different domains of the 30S ribosomal subunit which aids assembly (PubMed:17996707). http://togogenome.org/gene/300852:TTH_RS05345 ^@ http://purl.uniprot.org/uniprot/Q5SJF8 ^@ Function|||Similarity|||Subunit ^@ Belongs to the shikimate dehydrogenase family.|||Homodimer.|||Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA). http://togogenome.org/gene/300852:TTH_RS07920 ^@ http://purl.uniprot.org/uniprot/Q5SI16 ^@ Disruption Phenotype|||Function|||Similarity|||Subunit ^@ Belongs to the MGMT family. ATL subfamily.|||Disruption increases the spontaneous mutation rate.|||Interacts with several proteins, including UvrA, UvrD and the three subunits of the RNA polymerase.|||Involved in DNA damage recognition. Binds DNA containing O(6)-methylguanine and larger O(6)-alkylguanine adducts (PubMed:18483064, PubMed:23112169). Binds to the damaged base and flips the base out of the DNA duplex into an extrahelical conformation, which allows processing by repair proteins (PubMed:18483064). Also affects the regulation of gene expression in response to alkylation (PubMed:21531768). http://togogenome.org/gene/300852:TTH_RS05275 ^@ http://purl.uniprot.org/uniprot/Q5SJH2 ^@ Domain|||Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the GTP-binding SRP family. SRP54 subfamily.|||Composed of three domains: the N-terminal N domain, which is responsible for interactions with the ribosome, the central G domain, which binds GTP, and the C-terminal M domain, which binds the RNA and the signal sequence of the RNC.|||Cytoplasm|||Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY.|||Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY. http://togogenome.org/gene/300852:TTH_RS08260 ^@ http://purl.uniprot.org/uniprot/Q5SHU8 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the binding-protein-dependent transport system permease family.|||Cell membrane|||Membrane http://togogenome.org/gene/300852:TTH_RS07895 ^@ http://purl.uniprot.org/uniprot/Q5SI21 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the ArgR family.|||Cytoplasm|||Regulates arginine biosynthesis genes. http://togogenome.org/gene/300852:TTH_RS02740 ^@ http://purl.uniprot.org/uniprot/Q5SKW7 ^@ Function|||Similarity|||Subunit ^@ Belongs to the GcvP family. C-terminal subunit subfamily.|||The glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO(2) is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein.|||The glycine cleavage system is composed of four proteins: P, T, L and H. In this organism, the P 'protein' is a heterodimer of two subunits. http://togogenome.org/gene/300852:TTH_RS08445 ^@ http://purl.uniprot.org/uniprot/Q5SHR1 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the IF-1 family.|||Binds to the 30S ribosomal subunit, contacting protein S12 and the 16S rRNA (PubMed:11228145). Component of the 30S ribosomal translation pre-initiation complex which assembles on the 30S ribosome in the order IF-2 and IF-3, IF-1 and N-formylmethionyl-tRNA(fMet); mRNA recruitment can occur at any time during PIC assembly.|||Cytoplasm|||One of the essential components for the initiation of protein synthesis. Binds in the vicinity of the A-site (PubMed:11228145). Stabilizes the binding of IF-2 and IF-3 on the 30S subunit to which N-formylmethionyl-tRNA(fMet) subsequently binds. Helps modulate mRNA selection, yielding the 30S pre-initiation complex (PIC). Upon addition of the 50S ribosomal subunit IF-1, IF-2 and IF-3 are released leaving the mature 70S translation initiation complex. http://togogenome.org/gene/300852:TTH_RS04650 ^@ http://purl.uniprot.org/uniprot/Q8RR57 ^@ Function|||Miscellaneous|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Athough the Valle et al., electron microscopy paper indicates this protein came from T.thermophilus, its sequence maps to A.aeolicus (PubMed:12677067). The Fu et al., paper maps the coordinates of T.thermophilus to E.coli (PubMed:20940705).|||Belongs to the SmpB family.|||Binds tmRNA (PubMed:12677067, PubMed:20953161, PubMed:22422985, PubMed:17488812, PubMed:20940705, PubMed:22622583). Binds to both the 50S and 30S ribosomal subunits via rRNA contacts, bridges tmRNA binding to stalled ribosomes.|||Cytoplasm|||Required for rescue of stalled ribosomes mediated by trans-translation. Binds to tmRNA (also known as SsrA and 10Sa), required for stable association of tmRNA with ribosomes, probably by bridging tmRNA to 50S subunit. tmRNA and SmpB together mimic tRNA shape, replacing the anticodon stem-loop with SmpB (PubMed:22422985, PubMed:17488812). tmRNA is encoded by the ssrA gene; the 2 termini fold to resemble tRNA(Ala) and it encodes a 'tag peptide', a short internal open reading frame. During trans-translation Ala-aminoacylated tmRNA acts like a tRNA, entering the A-site of stalled ribosomes, displacing the stalled mRNA. The ribosome then switches to translate the ORF on the tmRNA; the nascent peptide is terminated with the 'tag peptide' encoded by the tmRNA and targeted for degradation. The ribosome is freed to recommence translation, which seems to be the essential function of trans-translation. http://togogenome.org/gene/300852:TTH_RS06730 ^@ http://purl.uniprot.org/uniprot/Q5SIP0 ^@ Activity Regulation|||Cofactor|||Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Activity increases by approximately two-fold in the presence of GCBP.|||Belongs to the glutamine synthetase family.|||Binds 2 Mg(2+) ions per subunit.|||Catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia.|||Cytoplasm|||Interacts with GCBP (TTHA1554). http://togogenome.org/gene/300852:TTH_RS02695 ^@ http://purl.uniprot.org/uniprot/Q5SKX6 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the DedA family.|||Cell membrane|||Membrane http://togogenome.org/gene/300852:TTH_RS04885 ^@ http://purl.uniprot.org/uniprot/Q5SJP7 ^@ Function|||Similarity|||Subunit ^@ Belongs to the non-flavoprotein flavin reductase family. HpaC subfamily.|||Catalyzes the reduction of free flavins (FMN, FAD and riboflavin) by NADH. Subsequently, the reduced flavins diffuse to the large HpaB component. It utilizes NADH, but not NADPH as an electron donor, and both FAD and FMN as electron acceptors.|||Homodimer. 4-HPA 3-monooxygenase consists of a reductase component HpaC and an oxygenase component HpaB. http://togogenome.org/gene/300852:TTH_RS05130 ^@ http://purl.uniprot.org/uniprot/Q5SJJ8 ^@ Caution|||Similarity|||Subcellular Location Annotation ^@ Belongs to the ABC-2 integral membrane protein family.|||Cell membrane|||Lacks conserved residue(s) required for the propagation of feature annotation.|||Membrane http://togogenome.org/gene/300852:TTH_RS07355 ^@ http://purl.uniprot.org/uniprot/Q5SIC1 ^@ Cofactor|||Similarity ^@ Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family.|||Binds 1 [2Fe-2S] cluster.|||Binds 1 [3Fe-4S] cluster.|||Binds 1 [4Fe-4S] cluster. http://togogenome.org/gene/300852:TTH_RS08965 ^@ http://purl.uniprot.org/uniprot/Q5SHG2 ^@ Cofactor|||Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Acetyl-CoA carboxylase is a heterohexamer composed of biotin carboxyl carrier protein (AccB), biotin carboxylase (AccC) and two subunits each of ACCase subunit alpha (AccA) and ACCase subunit beta (AccD).|||Belongs to the AccD/PCCB family.|||Binds 1 zinc ion per subunit.|||Component of the acetyl coenzyme A carboxylase (ACC) complex. Biotin carboxylase (BC) catalyzes the carboxylation of biotin on its carrier protein (BCCP) and then the CO(2) group is transferred by the transcarboxylase to acetyl-CoA to form malonyl-CoA.|||Cytoplasm http://togogenome.org/gene/300852:TTH_RS08855 ^@ http://purl.uniprot.org/uniprot/Q5SHI3 ^@ Similarity ^@ Belongs to the thioredoxin family. http://togogenome.org/gene/300852:TTH_RS06145 ^@ http://purl.uniprot.org/uniprot/Q5SJ04 ^@ Function|||Similarity|||Subunit ^@ Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily.|||Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-ketoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate).|||Homodimer. http://togogenome.org/gene/300852:TTH_RS05940 ^@ http://purl.uniprot.org/uniprot/Q5SJ43 ^@ Similarity ^@ Belongs to the bacterial solute-binding protein 3 family. http://togogenome.org/gene/300852:TTH_RS03800 ^@ http://purl.uniprot.org/uniprot/Q5SKB7 ^@ Similarity ^@ Belongs to the outer membrane factor (OMF) (TC 1.B.17) family. http://togogenome.org/gene/300852:TTH_RS04585 ^@ http://purl.uniprot.org/uniprot/Q5SJW3 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ Couples transcription and DNA repair by recognizing RNA polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent release of RNAP and its truncated transcript from the DNA, and recruitment of nucleotide excision repair machinery to the damaged site.|||Cytoplasm|||In the C-terminal section; belongs to the helicase family. RecG subfamily.|||In the N-terminal section; belongs to the UvrB family. http://togogenome.org/gene/300852:TTH_RS07750 ^@ http://purl.uniprot.org/uniprot/Q5SI49 ^@ Function|||Similarity ^@ Belongs to the pseudouridine synthase TruD family.|||Responsible for synthesis of pseudouridine from uracil-13 in transfer RNAs. http://togogenome.org/gene/300852:TTH_RS05695 ^@ http://purl.uniprot.org/uniprot/Q5SJ94 ^@ Function|||Similarity|||Subunit ^@ Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.|||Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate.|||Homodimer. http://togogenome.org/gene/300852:TTH_RS00110 ^@ http://purl.uniprot.org/uniprot/Q5SMC4 ^@ Similarity ^@ Belongs to the gamma-glutamylcyclotransferase family. http://togogenome.org/gene/300852:TTH_RS01770 ^@ http://purl.uniprot.org/uniprot/Q5SLF7 ^@ Function|||Similarity ^@ Belongs to the DMRL synthase family.|||Catalyzes the formation of 6,7-dimethyl-8-ribityllumazine by condensation of 5-amino-6-(D-ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin. http://togogenome.org/gene/300852:TTH_RS02080 ^@ http://purl.uniprot.org/uniprot/Q5SL95 ^@ Subcellular Location Annotation ^@ Membrane http://togogenome.org/gene/300852:TTH_RS08155 ^@ http://purl.uniprot.org/uniprot/Q5SHW9 ^@ Similarity ^@ Belongs to the 5-formyltetrahydrofolate cyclo-ligase family. http://togogenome.org/gene/300852:TTH_RS07075 ^@ http://purl.uniprot.org/uniprot/P80380 ^@ Function|||Similarity|||Subunit ^@ Belongs to the bacterial ribosomal protein bS20 family.|||One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the bottom of the body of the 30S subunit, by binding to several RNA helices of the 16S rRNA.|||Part of the 30S ribosomal subunit. http://togogenome.org/gene/300852:TTH_RS00915 ^@ http://purl.uniprot.org/uniprot/Q5SLW9 ^@ Cofactor|||Domain|||Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the DnaJ family.|||Binds 2 Zn(2+) ions per monomer.|||Cytoplasm|||Homodimer.|||Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins.|||The J domain is necessary and sufficient to stimulate DnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, DnaK-independent chaperone activity of DnaJ. Zinc center 2 is essential for interaction with DnaK and for DnaJ activity. http://togogenome.org/gene/300852:TTH_RS05250 ^@ http://purl.uniprot.org/uniprot/P60490 ^@ Function|||Similarity|||Subunit ^@ Belongs to the bacterial ribosomal protein bL19 family.|||Contacts the 16S rRNA of the 30S subunit (part of bridge B6), connecting the 2 subunits.|||Part of the 50S risobomal subunit. Contacts protein L14. Forms a bridge to the 30S subunit in the 70S ribosome, contacting the 16S rRNA. http://togogenome.org/gene/300852:TTH_RS00470 ^@ http://purl.uniprot.org/uniprot/Q56223 ^@ Cofactor|||Domain|||Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the complex I 75 kDa subunit family.|||Binds 1 [2Fe-2S] cluster per subunit. The [2Fe-2S] cluster 1 is referred to as N1b.|||Binds 3 [4Fe-4S] clusters per subunit. The [4Fe-4S] clusters 2, 3, and 4 are referred to as N5, N4, and N7, respectively. The [4Fe-4S] cluster 4 is too far away from the main redox chain to participate in electron transfer but probably confers structural stability.|||Cell membrane|||NDH-1 is composed of 15 different subunits, Nqo1 to Nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits Nqo7, Nqo8 and Nqo10 to Nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits Nqo1 to Nqo6, Nqo9 and Nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers.|||NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP.|||The subunit comprises two main parts, an N-terminal [FeFe]-hydrogenase-like domain (residues 1 to 240) that coordinates clusters 1, 2 and 3, and a domain similar to molybdopterin-containing enzymes (residues 241 to 767) whose first subdomain coordinates cluster 4. http://togogenome.org/gene/300852:TTH_RS02340 ^@ http://purl.uniprot.org/uniprot/Q5SL43 ^@ Subcellular Location Annotation ^@ Membrane http://togogenome.org/gene/300852:TTH_RS01915 ^@ http://purl.uniprot.org/uniprot/Q9LCX2 ^@ Function|||Similarity|||Subunit ^@ Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln).|||Belongs to the GatB/GatE family. GatB subfamily.|||Heterotrimer of A, B and C subunits. http://togogenome.org/gene/300852:TTH_RS08470 ^@ http://purl.uniprot.org/uniprot/Q5SHQ6 ^@ Similarity|||Subunit ^@ Belongs to the universal ribosomal protein uL30 family.|||Part of the 50S ribosomal subunit. http://togogenome.org/gene/300852:TTH_RS06860 ^@ http://purl.uniprot.org/uniprot/Q7SIA8 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the CutA family.|||Cytoplasm|||Homotrimer.|||Involved in resistance toward heavy metals. http://togogenome.org/gene/300852:TTH_RS07990 ^@ http://purl.uniprot.org/uniprot/Q5SI02 ^@ Similarity ^@ Belongs to the aldehyde dehydrogenase family. http://togogenome.org/gene/300852:TTH_RS00970 ^@ http://purl.uniprot.org/uniprot/Q5SLV8 ^@ Function ^@ Component of the pyruvate dehydrogenase (PDH) complex, that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). http://togogenome.org/gene/300852:TTH_RS04215 ^@ http://purl.uniprot.org/uniprot/Q5SK37 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.|||Membrane http://togogenome.org/gene/300852:TTH_RS03420 ^@ http://purl.uniprot.org/uniprot/Q84BQ9 ^@ Disruption Phenotype|||Domain|||Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the methyltransferase superfamily. PrmA family.|||Contains an N-terminal substrate recognition domain and a C-terminal methyltransferase domain, connected by a flexible linker.|||Cytoplasm|||Methylates ribosomal protein L11 (PubMed:15317787). Preferentially recognizes free L11 before its incorporation into 50S subunits (PubMed:15317787). This function is dispensable for growth and thermostability (PubMed:15317787).|||Mutant contains unmethylated protein L11 (PubMed:15317787). Null mutant is perfectly viable (PubMed:15317787). http://togogenome.org/gene/300852:TTH_RS06665 ^@ http://purl.uniprot.org/uniprot/Q5SIQ3 ^@ Cofactor|||Similarity|||Subcellular Location Annotation ^@ Belongs to the peptidase M50B family.|||Binds 1 zinc ion per subunit.|||Cell membrane|||Membrane http://togogenome.org/gene/300852:TTH_RS07005 ^@ http://purl.uniprot.org/uniprot/Q5SII6 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the bacterial secretin family.|||Cell outer membrane http://togogenome.org/gene/300852:TTH_RS07165 ^@ http://purl.uniprot.org/uniprot/Q5SIF4 ^@ Cofactor|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the SoxA family.|||Binds 2 heme groups per subunit.|||Heterodimer of SoxA and SoxX.|||Periplasm http://togogenome.org/gene/300852:TTH_RS04990 ^@ http://purl.uniprot.org/uniprot/Q5SJM6 ^@ Function|||Similarity ^@ Belongs to the bacterioferritin family.|||Iron-storage protein, whose ferroxidase center binds Fe(2+) ions, oxidizes them by dioxygen to Fe(3+), and participates in the subsequent Fe(3+) oxide mineral core formation within the central cavity of the protein complex. http://togogenome.org/gene/300852:TTH_RS03155 ^@ http://purl.uniprot.org/uniprot/Q5SKN6 ^@ Cofactor|||Function|||Similarity|||Subunit ^@ Belongs to the radical SAM superfamily. Biotin synthase family.|||Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.|||Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.|||Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.|||Homodimer. http://togogenome.org/gene/300852:TTH_RS08030 ^@ http://purl.uniprot.org/uniprot/Q5SHZ4 ^@ Cofactor|||Domain|||Function|||Miscellaneous|||Similarity|||Subcellular Location Annotation|||Subunit ^@ A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner (PubMed:23804759, PubMed:11850422). It probably also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes (PubMed:11850422). Relaxes negatively supercoiled DNA in an ATP-independent manner (PubMed:23804759, PubMed:11850422). At comparable concentrations T.thermophilus gyrase does not introduce as many negative supercoils into DNA as the E.coli enzyme (PubMed:23804759).|||Belongs to the type II topoisomerase GyrB family.|||Binds two Mg(2+) per subunit. The magnesium ions form salt bridges with both the protein and the DNA. Can also accept other divalent metal cations, such as Mn(2+) or Ca(2+).|||Consists of 3 domains; the ATPase domain (residues 1-220), the transducer domain (221-390) and the toprim domain (391-634) (PubMed:11850422). Removal of the N-terminal ATPase domain (residues 2-392) increases ATP-independent DNA relaxation, showing it is not required for DNA binding or cleavage, this enzyme still has some supercoiling activity, but in this case it introduces positive supercoils (PubMed:23804759).|||Cytoplasm|||Few gyrases are as efficient as E.coli at forming negative supercoils. Not all organisms have 2 type II topoisomerases; in organisms with a single type II topoisomerase this enzyme also has to decatenate newly replicated chromosomes.|||For the electron microscopy studies a GyrB-GyrA fusion protein was made with a Gly-Asp-Leu linker between the 2 subunits. It forms the expected dimer (PubMed:23804759).|||Heterotetramer, composed of two GyrA and two GyrB chains (PubMed:23804759). Non-hydrolyzable ATP analogs induce dimerization, novobiocin also induces a small amount of dimerization (PubMed:11850422). The two subunits form an intertwined dimer where the GyrB ATPase transducer helix of 1 subunit connects to the Toprim domain of the other GyrB subunit through a 10 residue linker (PubMed:23804759). In the heterotetramer, GyrA contains the active site tyrosine that forms a covalent intermediate with the DNA, while GyrB binds cofactors and catalyzes ATP hydrolysis.|||Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner. http://togogenome.org/gene/300852:TTH_RS08580 ^@ http://purl.uniprot.org/uniprot/P17291 ^@ Function|||Similarity|||Subunit ^@ Belongs to the universal ribosomal protein uS7 family.|||One of the primary rRNA binding proteins, it binds directly to 3'-end of the 16S rRNA where it nucleates assembly of the head domain of the 30S subunit. Is located at the subunit interface close to the decoding center. Binds mRNA and the E site tRNA blocking its exit path in the ribosome. This blockage implies that this section of the ribosome must be able to move to release the deacetylated tRNA.|||Part of the 30S ribosomal subunit. Contacts proteins S9 and S11. Binds to the C-terminus of IF3 and to the C-terminus of Era. http://togogenome.org/gene/300852:TTH_RS00090 ^@ http://purl.uniprot.org/uniprot/Q5SMC8 ^@ Cofactor|||Function|||Similarity ^@ Belongs to the IspH family.|||Binds 1 [4Fe-4S] cluster per subunit.|||Catalyzes the conversion of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate (HMBPP) into a mixture of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Acts in the terminal step of the DOXP/MEP pathway for isoprenoid precursor biosynthesis. http://togogenome.org/gene/300852:TTH_RS08490 ^@ http://purl.uniprot.org/uniprot/P0DOY9 ^@ Function|||Similarity|||Subunit ^@ Belongs to the universal ribosomal protein uS8 family.|||One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it helps nucleate assembly of the platform of the 30S subunit central domain. The combined cluster of proteins S8, S12 and S17 appears to hold together the shoulder and platform of the 30S subunit.|||Part of the 30S ribosomal subunit. Contacts proteins S2, S5 and S12. http://togogenome.org/gene/300852:TTH_RS08495 ^@ http://purl.uniprot.org/uniprot/P0DOY6 ^@ Cofactor|||Function|||Similarity|||Subunit ^@ Belongs to the universal ribosomal protein uS14 family. Zinc-binding uS14 subfamily.|||Binds 1 zinc ion per subunit.|||Part of the 30S ribosomal subunit. Forms a tight complex with proteins S3 and S10, which cover it.|||Required for the assembly of 30S particles and may also be responsible for determining the conformation of the 16S rRNA at the A site (By similarity). Binds 16S rRNA in center of the 30S subunit head. http://togogenome.org/gene/300852:TTH_RS06155 ^@ http://purl.uniprot.org/uniprot/Q5SJ02 ^@ Function|||Similarity|||Subunit ^@ Belongs to the acetolactate synthase small subunit family.|||Catalyzes the conversion of 2 pyruvate molecules into acetolactate in the first common step of the biosynthetic pathway of the branched-amino acids such as leucine, isoleucine, and valine.|||Dimer of large and small chains. http://togogenome.org/gene/300852:TTH_RS01075 ^@ http://purl.uniprot.org/uniprot/Q5SLT7 ^@ Similarity ^@ Belongs to the AlaDH/PNT family. http://togogenome.org/gene/300852:TTH_RS08035 ^@ http://purl.uniprot.org/uniprot/Q5SHZ3 ^@ Cofactor|||Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the D-alanine--D-alanine ligase family.|||Binds 2 magnesium or manganese ions per subunit.|||Cell wall formation.|||Cytoplasm http://togogenome.org/gene/300852:TTH_RS06810 ^@ http://purl.uniprot.org/uniprot/Q5SIM3 ^@ Similarity ^@ Belongs to the PGI/PMI family. http://togogenome.org/gene/300852:TTH_RS03950 ^@ http://purl.uniprot.org/uniprot/Q5SK87 ^@ Similarity ^@ Belongs to the SufE family. http://togogenome.org/gene/300852:TTH_RS07025 ^@ http://purl.uniprot.org/uniprot/Q5SII2 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.|||Cytoplasm|||Specifically methylates the cytosines at positions 1400 (m5C1400), 1404 (m5C1404) and 1407 (m5C1407) of 16S rRNA. C1400, C1404 and C1407 are methylated in a 30S subunit substrate, but only C1400 and C1404 are methylated when naked 16S rRNA is the substrate. Methylation by RsmF may facilitate growth at temperatures outside the optimal growth temperature. http://togogenome.org/gene/300852:TTH_RS02795 ^@ http://purl.uniprot.org/uniprot/Q5SKV7 ^@ Function|||Similarity ^@ Belongs to the LDH/MDH superfamily. MDH type 2 family.|||Catalyzes the reversible oxidation of malate to oxaloacetate. http://togogenome.org/gene/300852:TTH_RS01155 ^@ http://purl.uniprot.org/uniprot/Q5SLS1 ^@ Cofactor|||Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the adenylosuccinate synthetase family.|||Binds 1 Mg(2+) ion per subunit.|||Cytoplasm|||Homodimer.|||Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP. http://togogenome.org/gene/300852:TTH_RS00640 ^@ http://purl.uniprot.org/uniprot/Q5SM22 ^@ Similarity ^@ Belongs to the DprA/Smf family. http://togogenome.org/gene/300852:TTH_RS03915 ^@ http://purl.uniprot.org/uniprot/Q5SK94 ^@ Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the DtxR/MntR family.|||Cytoplasm|||Homodimer. http://togogenome.org/gene/300852:TTH_RS08350 ^@ http://purl.uniprot.org/uniprot/Q5SHT0 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the binding-protein-dependent transport system permease family.|||Cell membrane http://togogenome.org/gene/300852:TTH_RS04155 ^@ http://purl.uniprot.org/uniprot/Q5SK49 ^@ Function|||Similarity ^@ Belongs to the MqnA/MqnD family. MqnA subfamily.|||Catalyzes the dehydration of chorismate into 3-[(1-carboxyvinyl)oxy]benzoate, a step in the biosynthesis of menaquinone (MK, vitamin K2). http://togogenome.org/gene/300852:TTH_RS00445 ^@ http://purl.uniprot.org/uniprot/Q56218 ^@ Cofactor|||Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the complex I 20 kDa subunit family.|||Binds 1 [4Fe-4S] cluster per subunit. This [4Fe-4S] cluster is referred to as N2.|||Cell membrane|||NDH-1 is composed of 15 different subunits, Nqo1 to Nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits Nqo7, Nqo8 and Nqo10 to Nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits Nqo1 to Nqo6, Nqo9 and Nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers. This subunit interacts extensively with Nqo4.|||NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP. http://togogenome.org/gene/300852:TTH_RS04085 ^@ http://purl.uniprot.org/uniprot/Q5SK63 ^@ Similarity ^@ Belongs to the acyl-CoA dehydrogenase family. http://togogenome.org/gene/300852:TTH_RS08895 ^@ http://purl.uniprot.org/uniprot/Q5SHH5 ^@ Cofactor|||Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. LysJ subfamily.|||Binds 1 pyridoxal phosphate per subunit.|||Catalyzes the transfer of the amino group of L-glutamate to [LysW]-aminoadipate 6-semialdehyde, generating [LysW]-gamma-L-lysine.|||Cytoplasm|||Homodimer. http://togogenome.org/gene/300852:TTH_RS01295 ^@ http://purl.uniprot.org/uniprot/Q5SLP6 ^@ Function|||PTM|||Similarity|||Subunit ^@ Belongs to the universal ribosomal protein uL11 family.|||Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. The stalk extends beyond the surface of the 70S ribosome and is preferentially stabilized in 70S versus 50S crystals.|||In the 70S ribosome is in a position where it could interact transiently with the A site tRNA during translation.|||Part of the ribosomal stalk of the 50S ribosomal subunit. Interacts with L10 and the large rRNA to form the base of the stalk. L10 forms an elongated spine to which 3 L12 dimers bind in a sequential fashion forming a heptameric L10(L12)2(L12)2(L12)2 complex. Contacts one of the L7 dimers. Interacts with RF2 (release factor 2) (PubMed:18988853).|||The protein probably contains twelve methyl groups; at least one lysine residue (Lys-3) is known to be trimethylated. Methylation of the protein is not required for function. http://togogenome.org/gene/300852:TTH_RS03535 ^@ http://purl.uniprot.org/uniprot/Q5SKG5 ^@ Cofactor|||Function|||Similarity ^@ Belongs to the ThiC family.|||Binds 1 [4Fe-4S] cluster per subunit. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.|||Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction. http://togogenome.org/gene/300852:TTH_RS00480 ^@ http://purl.uniprot.org/uniprot/Q56224 ^@ Cofactor|||Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the complex I 23 kDa subunit family.|||Binds 2 [4Fe-4S] clusters per subunit. The [4Fe-4S] clusters are referred to as N6a and N6b.|||Cell membrane|||NDH-1 is composed of 15 different subunits, Nqo1 to Nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits Nqo7, Nqo8 and Nqo10 to Nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits Nqo1 to Nqo6, Nqo9 and Nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers.|||NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP. The role of the Nqo9 subunit appears to provide a 'connecting chain' of two clusters between cluster N5 and the terminal cluster N2, and to stabilize the structure of the complex by interacting with other subunits. http://togogenome.org/gene/300852:TTH_RS01395 ^@ http://purl.uniprot.org/uniprot/Q5SLN0 ^@ Subcellular Location Annotation ^@ Membrane http://togogenome.org/gene/300852:TTH_RS03830 ^@ http://purl.uniprot.org/uniprot/Q5SKB1 ^@ Similarity ^@ Belongs to the DapA family. http://togogenome.org/gene/300852:TTH_RS07940 ^@ http://purl.uniprot.org/uniprot/Q5SI12 ^@ Function|||Miscellaneous|||Similarity ^@ Belongs to the MqnA/MqnD family. MqnD subfamily.|||Catalyzes the conversion of cyclic dehypoxanthine futalosine (cyclic DHFL) into 1,4-dihydroxy-6-naphthoate, a step in the biosynthesis of menaquinone (MK, vitamin K2).|||The tartarate seen in the active site in the crystallographic structure may partially mimic the substrate; it is suspected that the tartarate may resemble the open-chain form of the ribose part of the substrate, cyclic dehypoxanthine futalosine. http://togogenome.org/gene/300852:TTH_RS00245 ^@ http://purl.uniprot.org/uniprot/Q56232 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.|||Catalyzes the reversible conversion of aspartate and 2-oxoglutarate to glutamate and oxaloacetate (PubMed:8907187, PubMed:25070637). Can also transaminate prephenate in the presence of aspartate (PubMed:25070637, PubMed:30771275).|||Cytoplasm|||Homodimer. http://togogenome.org/gene/300852:TTH_RS07225 ^@ http://purl.uniprot.org/uniprot/Q5SIE7 ^@ Similarity ^@ Belongs to the helicase family. UvrD subfamily. http://togogenome.org/gene/300852:TTH_RS03200 ^@ http://purl.uniprot.org/uniprot/Q5SKM9 ^@ Domain|||Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the FKBP-type PPIase family. Tig subfamily.|||Consists of 3 domains; the N-terminus binds the ribosome, the middle domain has PPIase activity, while the C-terminus has intrinsic chaperone activity on its own.|||Cytoplasm|||Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation. Functions as a peptidyl-prolyl cis-trans isomerase. http://togogenome.org/gene/300852:TTH_RS05515 ^@ http://purl.uniprot.org/uniprot/Q5SJD1 ^@ Subcellular Location Annotation ^@ Membrane http://togogenome.org/gene/300852:TTH_RS03700 ^@ http://purl.uniprot.org/uniprot/Q5SKD2 ^@ Function|||Induction|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the class-II aminoacyl-tRNA synthetase family. Type 1 subfamily.|||Catalyzes the attachment of L-aspartate to tRNA(Asp) in a two-step reaction: L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp). Is specific for tRNA(Asp) since it aspartylates tRNA(Asn) 3 orders of magnitude less efficiently than tRNA(Asp).|||Constitutively expressed in a constant ratio along the growth of the bacterium.|||Cytoplasm|||Homodimer. http://togogenome.org/gene/300852:TTH_RS09240 ^@ http://purl.uniprot.org/uniprot/Q5SHB0 ^@ Similarity ^@ Belongs to the CinA family. http://togogenome.org/gene/300852:TTH_RS05560 ^@ http://purl.uniprot.org/uniprot/Q5SJC2 ^@ Cofactor|||Function|||Similarity ^@ Belongs to the radical SAM superfamily. MqnC family.|||Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.|||Radical SAM enzyme that catalyzes the cyclization of dehypoxanthine futalosine (DHFL) into cyclic dehypoxanthine futalosine (CDHFL), a step in the biosynthesis of menaquinone (MK, vitamin K2). http://togogenome.org/gene/300852:TTH_RS02305 ^@ http://purl.uniprot.org/uniprot/Q5SL50 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the OXA1/ALB3/YidC family. Type 1 subfamily.|||Cell inner membrane|||Interacts with the Sec translocase complex via SecD. Specifically interacts with transmembrane segments of nascent integral membrane proteins during membrane integration.|||Membrane|||Required for the insertion and/or proper folding and/or complex formation of integral membrane proteins into the membrane. Involved in integration of membrane proteins that insert both dependently and independently of the Sec translocase complex, as well as at least some lipoproteins. Aids folding of multispanning membrane proteins. http://togogenome.org/gene/300852:TTH_RS06175 ^@ http://purl.uniprot.org/uniprot/Q5SIZ7 ^@ Subcellular Location Annotation ^@ Cell inner membrane|||Cell outer membrane|||Membrane|||Periplasm http://togogenome.org/gene/300852:TTH_RS00975 ^@ http://purl.uniprot.org/uniprot/Q5SLV7 ^@ Similarity ^@ Belongs to the LysR transcriptional regulatory family. http://togogenome.org/gene/300852:TTH_RS04230 ^@ http://purl.uniprot.org/uniprot/Q5SK33 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.|||Membrane http://togogenome.org/gene/300852:TTH_RS00620 ^@ http://purl.uniprot.org/uniprot/Q5SM25 ^@ Cofactor|||Disruption Phenotype|||Function|||Similarity|||Subunit ^@ Belongs to the NrnA oligoribonuclease family.|||Bifunctional enzyme which has both oligoribonuclease and pAp-phosphatase activities. Degrades short RNA and DNA oligonucleotides with a length of up to 33 nucleotides, although the enzyme is most active on shorter substrates, in a 5' to 3' direction. Converts 3'(2')-phosphoadenosine 5'-phosphate (PAP) to AMP, has very low activity on cAMP and cGMP.|||Oligomeric.|||Reduced growth in minimal medium, possibly also affected in cysteine assimilation.|||Requires a divalent cation; Co(2+) > Mn(2+) >Zn(2+) > Mg(2+). http://togogenome.org/gene/300852:TTH_RS03560 ^@ http://purl.uniprot.org/uniprot/Q5SKG0 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the ExbD/TolR family.|||Cell membrane http://togogenome.org/gene/300852:TTH_RS00435 ^@ http://purl.uniprot.org/uniprot/Q5SM60 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily.|||Cytoplasm|||Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits. http://togogenome.org/gene/300852:TTH_RS05575 ^@ http://purl.uniprot.org/uniprot/Q5SJB9 ^@ Function|||Similarity|||Subunit ^@ Belongs to the TrpB family.|||Tetramer of two alpha and two beta chains.|||The beta subunit is responsible for the synthesis of L-tryptophan from indole and L-serine. http://togogenome.org/gene/300852:TTH_RS07015 ^@ http://purl.uniprot.org/uniprot/Q5SII4 ^@ Cofactor|||Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the shikimate kinase family.|||Binds 1 Mg(2+) ion per subunit.|||Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate.|||Cytoplasm|||Monomer. http://togogenome.org/gene/300852:TTH_RS07755 ^@ http://purl.uniprot.org/uniprot/Q5SI48 ^@ Cofactor|||Similarity ^@ Belongs to the peptidase M3 family.|||Binds 1 zinc ion. http://togogenome.org/gene/300852:TTH_RS00275 ^@ http://purl.uniprot.org/uniprot/Q5SM91 ^@ Similarity ^@ Belongs to the histone deacetylase family. http://togogenome.org/gene/300852:TTH_RS09670 ^@ http://purl.uniprot.org/uniprot/Q5SH23 ^@ Cofactor|||Function|||Similarity|||Subunit ^@ Belongs to the RimK family. LysX subfamily.|||Binds 2 magnesium ions per subunit.|||Catalyzes the ATP-dependent formation of a covalent bond between the amino group of alpha-aminoadipate (AAA) and the gamma-carboxyl group of the C-terminal glutamate residue in LysW.|||Homodimer. http://togogenome.org/gene/300852:TTH_RS06170 ^@ http://purl.uniprot.org/uniprot/Q5SIZ8 ^@ Subcellular Location Annotation ^@ Cell inner membrane|||Cell outer membrane|||Membrane|||Periplasm http://togogenome.org/gene/300852:TTH_RS03385 ^@ http://purl.uniprot.org/uniprot/Q5SKJ4 ^@ Subcellular Location Annotation ^@ Membrane http://togogenome.org/gene/300852:TTH_RS04135 ^@ http://purl.uniprot.org/uniprot/Q5SK53 ^@ Similarity ^@ Belongs to the prephenate/arogenate dehydrogenase family. http://togogenome.org/gene/300852:TTH_RS08810 ^@ http://purl.uniprot.org/uniprot/Q5SHJ0 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.|||Cytoplasm http://togogenome.org/gene/300852:TTH_RS04295 ^@ http://purl.uniprot.org/uniprot/Q5SK21 ^@ Similarity ^@ Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. http://togogenome.org/gene/300852:TTH_RS06855 ^@ http://purl.uniprot.org/uniprot/Q5SIL4 ^@ Function|||Miscellaneous|||Similarity|||Subcellular Location Annotation|||Subunit ^@ A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state (PubMed:23804759, PubMed:11850422). It probably also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes (PubMed:11850422). Relaxes negatively supercoiled DNA in an ATP-independent manner (PubMed:23804759, PubMed:11850422). At comparable concentrations T.thermophilus gyrase does not introduce as many negative supercoils into DNA as the E.coli enzyme (PubMed:23804759).|||Belongs to the type II topoisomerase GyrA/ParC subunit family.|||Cytoplasm|||Few gyrases are as efficient as E.coli at forming negative supercoils. Not all organisms have 2 type II topoisomerases; in organisms with a single type II topoisomerase this enzyme also has to decatenate newly replicated chromosomes.|||For the electron microscopy studies a GyrB-GyrA fusion protein was made with a Gly-Asp-Leu linker between the 2 subunits. It forms the expected dimer (PubMed:23804759).|||Heterotetramer, composed of two GyrA and two GyrB chains (PubMed:23804759). The two subunits form an intertwined dimer where the GyrB ATPase transducer helix of 1 subunit connects to the Toprim domain of the other GyrB subunit through a 10 residue linker (PubMed:23804759). In the heterotetramer, GyrA contains the active site tyrosine that forms a transient covalent intermediate with the DNA, while GyrB binds cofactors and catalyzes ATP hydrolysis.|||Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner. http://togogenome.org/gene/300852:TTH_RS06785 ^@ http://purl.uniprot.org/uniprot/Q5SIM8 ^@ Subcellular Location Annotation ^@ Membrane http://togogenome.org/gene/300852:TTH_RS01780 ^@ http://purl.uniprot.org/uniprot/Q5SLF5 ^@ Function|||Similarity|||Subunit ^@ Belongs to the SAM hydrolase / SAM-dependent halogenase family.|||Catalyzes the hydrolysis of S-adenosyl-L-methionine (SAM) into adenosine and L-methionine (PubMed:36996195). Does not have chlorinase or fluorinase activity (PubMed:36996195).|||Homotrimer.